Sanguinarine inhibits Rac1b-rendered cell survival enhancement by promoting apoptosis and blocking proliferation

Acta Pharmacologica Sinica

Volumn 36, Issue 2, 2015, Pages 229-240

  Ying, Li ^a,b   Li, Gang ^c   Wei, Si Si ^c   Wang, Hong ^a   An, Pei ^a   Wang, Xun ^a   Guo, Kai ^a   Luo, Xian Jin ^c   Gao, Ji Min ^d   Zhou, Qing ^a   Li, Wei ^a   Yu, Ying ^a   Li, Yi Gang ^a   Duan, Jun Li ^b   Wang, Yue Peng ^a  

^a TENTH PEOPLE S HOSPITAL OF TONGJI UNIVERSITY   (China)

^b SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

^c SHANGHAI JIAO TONG UNIVERSITY   (China)

^d BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

Author keywords

anticancer drug; berberine; cell proliferation; HEK293T cell; Rac1b; sanguinarine; small GTPase

Indexed keywords

ANTINEOPLASTIC AGENT; BERBERINE; BROXURIDINE; CYCLIN D1; GREEN FLUORESCENT PROTEIN; PARP 89; PROTEIN; RAC1 PROTEIN; RAC1B PROTEIN; SANGUINARINE; UNCLASSIFIED DRUG; BENZOPHENANTHRIDINE DERIVATIVE; ISOQUINOLINE DERIVATIVE; RAC1 PROTEIN, HUMAN;

APOPTOSIS; ARTICLE; CELL ASSAY; CELL COUNT; CELL GROWTH; CELL PROLIFERATION; CELL SURVIVAL; CELLULAR PARAMETERS; CONTROLLED STUDY; DRUG SELECTIVITY; DRUG STRUCTURE; G1 PHASE CELL CYCLE CHECKPOINT; INCORPORATION ASSAY; INFECTION; MALIGNANT NEOPLASTIC DISEASE; PROLIFERATIVE INDEX; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; UPREGULATION; WESTERN BLOTTING; CELL LINE; DRUG EFFECTS; HEK293 CELL LINE; HUMAN; METABOLISM;

APOPTOSIS; BENZOPHENANTHRIDINES; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; HEK293 CELLS; HUMANS; ISOQUINOLINES; RAC1 GTP-BINDING PROTEIN;

EID: 84922333519     PISSN: 16714083     EISSN: 17457254     Source Type: Journal    
DOI: 10.1038/aps.2014.115     Document Type: Article

References (29)

1. Jaffe AB, Hall A. Rho GTPases: Biochemistry and biology. Annu Rev Cell Dev Biol 2005; 21: 247-69.
2. Hall A. Rho family GTPases. Biochem Soc Trans 2012; 40: 1378-82.
3. Burridge K, Wennerberg K. Rho and Rac take center stage. Cell 2004; 116: 167-79.
4. Caron E, Hall A. Identification of two distinct mechanisms of phagocytosis controlled by different Rho GTPases. Science 1998; 282: 1717-21.
5. Schnelzer A, Prechtel D, Knaus U, Dehne K, Gerhard M, Graeff H, et al. Rac1 in human breast cancer: overexpression, mutation analysis, and characterization of a new isoform, Rac1b. Oncogene 2000; 19: 3013-20.
6. Jordan P, Brazao R, Boavida MG, Gespach C, Chastre E. Cloning of a novel human Rac1b splice variant with increased expression in colorectal tumors. Oncogene 1999; 18: 6835-9.
7. Karlsson R, Pedersen ED, Wang Z, Brakebusch C. Rho GTPase function in tumorigenesis. Biochim Biophys Acta 2009; 1796: 91-8.
8. Choi SS, Sicklick JK, Ma Q, Yang L, Huang J, Qi Y, et al. Sustained activation of rac1 in hepatic stellate cells promotes liver injury and fibrosis in mice. Hepatology 2006; 44: 1267-77.
9. Boettner B, Van Aelst L. The role of Rho GTPases in disease development. Gene 2002; 286: 155-74.
10. Schenck A, Bardoni B, Moro A, Bagni C, Mandel JL. A highly conserved protein family interacting with the fragile X mental retardation protein (FMRP) and displaying selective interactions with FMRP-related proteins FXR1P and FXR2P. Proc Natl Acad Sci U S A 2001; 98: 8844-9.
11. Keely PJ, Westwick JK, Whitehead IP, Der CJ, Parise LV. Cdc42 and Rac1 induce integrin-mediated cell motility and invasiveness through PI(3)K. Nature 1997; 390: 632-5.
12. Anand-Apte B, Zetter BR, Viswanathan A, Qiu RG, Chen J, Ruggieri R, et al. Platelet-derived growth factor and fibronectin-stimulated migration are differentially regulated by the Rac and extracellular signal-regulated kinase pathways. J Biol Chem 1997; 272: 30688-92.
13. Fiegen D, Haeusler LC, Blumenstein L, Herbrand U, Dvorsky R, Vetter IR, et al. Alternative splicing of Rac1 generates Rac1b, a selfactivating GTPase. J Biol Chem 2004; 279: 4743-9.
14. Singh A, Karnoub AE, Palmby TR, Lengyel E, Sondek J, Der CJ. Rac1b, a tumor associated, constitutively active Rac1 splice variant, promotes cellular transformation. Oncogene 2004; 23: 9369-80.
15. Matos P, Collard JG, Jordan P. Tumor-related alternatively spliced Rac1b is not regulated by Rho-GDP dissociation inhibitors and exhibits selective downstream signaling. J Biol Chem 2003; 278: 50442-8.
16. Stallings-Mann M, Radisky D. Matrix metalloproteinase-induced malignancy in mammary epithelial cells. Cells Tissues Organs 2007; 185: 104-10.
17. Liu J, Lee W, Jiang Z, Chen Z, Jhunjhunwala S, Haverty PM, et al. Genome and transcriptome sequencing of lung cancers reveal diverse mutational and splicing events. Genome Res 2012; 22: 2315-27.
18. Silva AL, Carmo F, Bugalho MJ. RAC1b overexpression in papillary thyroid carcinoma: a role to unravel. Eur J Endocrinol 2013; 168: 795-804.
19. Matos P, Jordan P. Expression of Rac1b stimulates NF-?B-mediated cell survival and G1/S progression. Exp Cell Res 2005; 305: 292-9.
20. Beausoleil E, Chauvignac C, Taverne T, Lacombe S, Pognante L, Leblond B, et al. Structure-activity relationship of isoform selective inhibitors. Bioorg Med Chem Lett 2009; 19: 5594-8.
21. Wang YP, Zheng XR, Riddick N, Bryden M, Baur W, Zhang X, et al. ROCK isoform regulation of myosin phosphatase and contractility in vascular smooth muscle cells. Circ Res 2009; 104: 531-40.
22. Wang YP, Ei-Zaru MR EZ, Surks HK, Mendelsohn ME. Formin homology domain protein FHOD1 is a cyclic GMP-dependent protein kinase Ia binding protein and substrate in vascular smooth muscle cells. J Biol Chem 2004; 279: 24420-6.
23. Wang YP, Chen J, Wang Y, Taylor CW, Hirata Y, Hagiwara H, et al. Crucial role of type-1, but not type-3, inositol 1,4,5-trisphosphate (IP3) receptors in IP3-induced Ca2+ release, capacitative Ca2+ entry, and proliferation of A7r5 vascular smooth muscle cells. Cir Res 2001; 88: 202-9.
24. Adhami VM, Aziz MH, Mukhtar H, Ahmad N. Activation of prodeath Bcl-2 family proteins and mitochondrial apoptosis pathway by sanguinarine in immortalized human HaCaT keratinocytes. Clin Cancer Res 2003; 9: 3176-82.
25. Adhami VM, Aziz MH, Reagan-Shaw SR, Nihal M, Mukhtar H, Ahmad N. Sanguinarine causes cell cycle blockade and apoptosis of human prostate carcinoma cells via modulation of cyclin kinase inhibitorcyclin-cyclin-dependent kinase machinery. Mol Cancer Ther 2004; 3: 933-40.
26. Malikova J, Zdarilova A, Hlobilkova A, UlrichováJ. The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine. Cell Biol Toxicol 2006; 22: 439-53.
27. Han MH, Kim GY, Yoo YH, Choi YH. Sanguinarine induces apoptosis in human colorectal cancer HCT-116 cells through ROS-mediated Egr-1 activation and mitochondrial dysfunction. Toxicol Lett 2013; 220: 157-66.
28. Maiti M, Kumar GS. Polymorphic nucleic acid binding of bioactive isoquinoline alkaloids and their role in cancer. J Nucleic Acids 2010; 2010: 1-23.
29. Bhadra K, Kumar GS. Therapeutic potential of nucleic acid-binding isoquinoline alkaloids: Binding aspects and implications for drug design. Med Res Rev 2010; 31: 821-62.