A Three-Step Assay for Ceramide Synthase Activity Using a Fluorescent Substrate and HPLC

Lipids

Volumn 50, Issue 1, 2014, Pages 101-109

  Couttas, Timothy A ^a   Lim, Xin Y ^a   Don, Anthony S ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

Ceramide; Ceramide synthase; CERS; Dihydrosphingosine; Fluorescent assay; HPLC; Method; Sphingolipid

Indexed keywords

CERAMIDE; CHLOROFORM; COENZYME A; FATTY ACID; METHANOL; MYELIN; SYNTHETASE; VERY LONG CHAIN FATTY ACID; CERS2 PROTEIN, HUMAN; FLUORESCENT DYE; MEMBRANE PROTEIN; SPHINGOSINE ACYLTRANSFERASE; TUMOR SUPPRESSOR PROTEIN;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; FLUORESCENCE; GRAY MATTER; HEK293 CELL LINE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN TISSUE; LIMIT OF DETECTION; LIMIT OF QUANTITATION; MOUSE; NONHUMAN; REPRODUCIBILITY; REVERSED PHASE LIQUID CHROMATOGRAPHY; THIN LAYER CHROMATOGRAPHY; ENZYME ASSAY; ENZYME SPECIFICITY; EVALUATION STUDY; METABOLISM; PROCEDURES; TUMOR CELL LINE;

CELL LINE, TUMOR; CERAMIDES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME ASSAYS; FLUORESCENCE; FLUORESCENT DYES; HEK293 CELLS; HUMANS; LIMIT OF DETECTION; MEMBRANE PROTEINS; REPRODUCIBILITY OF RESULTS; SPHINGOSINE N-ACYLTRANSFERASE; SUBSTRATE SPECIFICITY; TUMOR SUPPRESSOR PROTEINS;

EID: 84922105648     PISSN: 00244201     EISSN: 15589307     Source Type: Journal    
DOI: 10.1007/s11745-014-3969-5     Document Type: Article

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