Small indels induced by CRISPR/Cas9 in the 5′ region of microRNA lead to its depletion and Drosha processing retardance

RNA Biology

Volumn 11, Issue 10, 2014, Pages 1243-1249

  Jiang, Qian ^a   Meng, Xing ^a   Meng, Lingwei ^a   Chang, Nannan ^a   Xiong, Jingwei ^a   Cao, Huiqing ^a   Liang, Zicai ^a,b  

^a PEKING UNIVERSITY   (China)

^b Chemical Engineering Research Center   (China)

Author keywords

CRISPR Cas9; MicroRNA biogenesis; MicroRNA depletion; MiR 93; RNA guided dna cleavage

Indexed keywords

CAS9 PROTEIN; DROSHA PROTEIN; MICRORNA; MICRORNA 93; NUCLEOTIDE; PROTEIN; UNCLASSIFIED DRUG; DROSHA PROTEIN, HUMAN; MESSENGER RNA; MIRN93 MICRORNA, HUMAN; RIBONUCLEASE III; RNA PRECURSOR;

5' UNTRANSLATED REGION; ALLELE; ARTICLE; BIOGENESIS; CELL PROLIFERATION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; FEMALE; HELA CELL LINE; HUMAN; HUMAN CELL; INDEL MUTATION; KNOCKOUT GENE; PHENOTYPE; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROCESSING; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA SEQUENCE; RNA STRUCTURE; UPREGULATION; CONFORMATION; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; RNA PROCESSING; WESTERN BLOTTING;

BASE SEQUENCE; BLOTTING, WESTERN; CELL PROLIFERATION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; HELA CELLS; HUMANS; INDEL MUTATION; MICRORNAS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RIBONUCLEASE III; RNA PRECURSORS; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, MESSENGER;

EID: 84921929541     PISSN: 15476286     EISSN: 15558584     Source Type: Journal    
DOI: 10.1080/15476286.2014.996067     Document Type: Article

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