Cell-based protein stabilization assays for the detection of interactions between small-molecule inhibitors and BRD4

Journal of Biomolecular Screening

Volumn 20, Issue 2, 2015, Pages 180-189

  Schulze, Jessica ^a,c   Moosmayer, Dieter ^a   Weiske, Joerg ^a   Fernández Montalván, Amaury ^a   Herbst, Christopher ^a   Jung, Marie ^a,b   Haendler, Bernard ^a   Bader, Benjamin ^a  

^a BAYER PHARMA AG   (Germany)

^b FREIE UNIVERSITÄT BERLIN   (Germany)

^c MAX PLANCK INSTITUTE OF COLLOIDS AND INTERFACES   (Germany)

Author keywords

BRD4; bromodomain; cell based assays; EFC; TR FRET

Indexed keywords

BROMODOMAIN INHIBITOR; BROMODOMAIN PROTEIN 4; LIGAND; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; BRD4 PROTEIN, HUMAN; MOLECULAR LIBRARY; NUCLEAR PROTEIN;

ARTICLE; BINDING ASSAY; CANCER GROWTH; CANCER INHIBITION; CELL CULTURE; CELL LYSATE; CELL PROLIFERATION; CONTROLLED STUDY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; HUMAN CELL; MEDICAL TECHNOLOGY; MUTANT; POINT MUTATION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN FAMILY; PROTEIN INTERACTION; PROTEIN STABILITY; TRANSCRIPTION ELONGATION; ANTAGONISTS AND INHIBITORS; CELL LINE; DRUG DEVELOPMENT; DRUG EFFECTS; FLUOROIMMUNOASSAY; GENETICS; HIGH THROUGHPUT SCREENING; METABOLISM; MOLECULAR LIBRARY; MUTATION; PROCEDURES; REPRODUCIBILITY; STANDARDS;

CELL LINE; DRUG DISCOVERY; FLUOROIMMUNOASSAY; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; LIGANDS; MUTATION; NUCLEAR PROTEINS; PROTEIN STABILITY; REPRODUCIBILITY OF RESULTS; SMALL MOLECULE LIBRARIES; TRANSCRIPTION FACTORS;

EID: 84921481125     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057114552398     Document Type: Article

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