β galactosidase complementation: A cell-based luminescent assay platform for drug discovery

Assay and Drug Development Technologies

Volumn 5, Issue 1, 2007, Pages 137-144

  Olson, Keith R ^a   Eglen, Richard M ^a,b  

^a DiscoveRx Corporation   (United States)

^b PERKINELMER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE;

CYTOLOGY; ENZYME ASSAY; ENZYME SUBSTRATE; HIGH THROUGHPUT SCREENING; LUMINESCENCE; REVIEW; SIGNAL TRANSDUCTION; TECHNOLOGY;

BETA-GALACTOSIDASE; BIOLOGICAL ASSAY; CHEMILUMINESCENT MEASUREMENTS; DRUG DESIGN; DRUG EVALUATION, PRECLINICAL; MICROSCOPY, FLUORESCENCE;

EID: 34147207553     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/adt.2006.052     Document Type: Review

References (46)

1. Lang P, Yeow K, Nichols A, Scheer A: Cellular imaging in drug discovery. Nat Rev Drug Discov 2006;5:343-356.
2. Cregger M, Berger AJ, Rimm DL: Immunohistochemistry and quantitative analysis of protein expression. Arch Pathol Lab Med 2006;130:1026-1030.
3. Giuliano KA, Haskins JR, Taylor DL: Advances in high content screening for drug discovery. Assay Drug Dev Technol 2003;1:565-577.
4. Liu HS, Jan MS, Chou CK, Chen PH, Ke NJ: Is green fluorescent protein toxic to the living cells? Biochem Biophys Res Commun 1999;260:712-717.
5. Yu H, West M, Keon BH, Bilter GK, Owens S, Lamerdin J, et al.: Measuring drug action in the cellular context using protein-fragment complementation assays. Assay Drug Dev Technol 2003;1:811-822.
6. MacDonald M, Lamerdin J, Owens S, Keon BH, Bilter GK, Shang Z, et al.: Identifying off-target effects and hidden phenotypes of drugs in human cells. Nat Chem Biol 2006;2:329-337.
7. Michnick SW: Exploring protein interactions by interaction-induced folding of proteins from complementary peptide fragments. Curr Opin Struct Biol 2001;11:472-477.
8. Michnick SW: Protein fragment complementation strategies for biochemical network mapping. Curr Opin Biotechnol 2003;14:610-617.
9. Ullmann A, Jacob F, Monod J: Characterization by an in vitro complementation of a peptide corresponding to an operator-proximal segment of β-galactosidase structural gene of Escherichia coli. J Mol Biol 1967;24:339-348.
10. Zabin Z, Villarejo MR: Protein complementation. Annu Rev Biochem 1975;44:295-313.
11. Langley KE, Zabin I: β-Galactosidase α complementation: properties of the complemented enzyme and mechanism of the complementation reaction. Biochemistry 1976;15:4866-4875.
12. Juers DH, Jacobson RH, Wigley D, Zhang X-J, Huber RE, Tronrud DE, et al.: High resolution refinement of β-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation. Protein Sci 2000;9:1685-1699.
13. Marinkovic DV, Marinkovich JN: Restoration of β-galactosidase to E. coli M15: complementation studies. Biochem J 1997;165:417-423.
14. Zamenhof PJ, Villarejo M: Construction and properties of Escherichia coli strains exhibiting α-complementation of β-galactosidase fragments in vivo. J Bacteriol 1972;110:171-178.
15. Nichtl A, Buchner J, Jaenicke R, Rudolph R, Scheibel T: Folding and association of β-galactosidase. J Mol Biol 1998;282:1083-1091.
16. Eglen RM, Singh R: β-Galactosidase enzyme fragment complementation as a novel technology for high throughput screening. Comb Chem HTS 2003;6:313-319.
17. Coty WA, Loor R, Powell M, Khanna PL: CEDIA® homogeneous immunoassays: current status and future prospects. J Clin Immunoassay 1994;17:144-150.
18. Khanna PL, Coty WA: Cloned enzyme donor immunoassay. In: Methods of Immunological Analysis, Vol. 1 (Masseyeff RF, Albert WH, Staines NA, eds.), pp. 416-432. VCH Verlagsgesellschaft MbH, Weinheim, Germany, 1993.
19. Gosling JP: Enzyme immunoassay: with and without separation. In: Principles and Practice of Immunoassay, 2nd ed. (Price CP, Newman DJ, eds.), pp. 351-388. Stockton Press, New York, 1997.
20. Eglen RM: Enzyme fragment complementation: a flexible high throughput screening assay technology. Assay Drug Dev Technol 2002;1:97-103.
21. Williams C: cAMP detection methods in HTS: selecting the best from the rest. Nat Rev Drug Discov 2003;3:125-134.
22. Golla R, Seethala R: A homogeneous enzyme fragment complementation cyclic AMP screen for GPCR agonists. J Biomol Screen 2002;7:515-525.
23. Weber M, Ferrer M, Zheng W, Inglese J, Strulovici B, Kunapuli PA: 1536 well cAMP assay for Gs and Gi coupled receptors using enzyme fragment complementation. Assay Drug Dev Technol 2004;1:39-49.
24. Vainshtein I, Silveria S, Kaul P, Rouhani R, Eglen RM, Wang J: A high-throughput, nonisotopic, competitive binding assay for kinases using nonselective inhibitor probes (ED-NSIP). J Biomol Screen 2002;7:497-506.
25. Mooseman O, Rusconi S: α-Complementation of Lac Z in mammalian cells. Nucleic Acids Res 1996;24:1171-1172.
26. Homma T, Yoshihisa T, Kihara A, Akiyama Y, Ito K: Intracellular stability of a fragments of β galactosidase: effects of amino-terminally fused polypeptides. Biochem Biophys Res Commun 1995;215:452-458.
27. Kurien BT, Scofield RH: Protein blotting: a review. J Immunol Methods 2003;274:1-15.
28. Horecka J, Charter NW, Bosano B, Fung P, Peng K, Eglen RM: A novel antibody-free method for protein blotting using enzyme fragment complementation (EFC). Biotechniques 2006;40:381-383.
29. Wigley CW, Stidham RD, Smith NM, Hunt JF, Thomas PJ: Protein solubility and folding monitored in vivo by structural complementation of a genetic marker protein. Nat Biotechnol 2001;19:131-136.
30. Lamarcq LH, Scherer BJ, Phelan ML, Kalnine NN, Nguyen YH, Kabakova T, et al.: Large-scale, high-throughput validation of short hairpin RNA sequences for RNA interference. J Biomol Screen 2006;11:236-246.
31. Abbas-Terki T, Picard D: α-Complemented β-galactosidase: an in vivo model substrate for the molecular chaperone heat-shock protein 90 in yeast. Eur J Biochem 1999;266:517-523.
32. Horecka J, Eglen RM: A homogeneous assay to measure nuclear hormone receptor degradation using β galactosidase complementation [abstract P06012]. In: Abstracts, XIth Society for Biomolecular Screening, Geneva, September 11-15, 2005. Danbury, CT: Society for Biomolecular Screening, 2005.
33. Zhao X, Vainshtein V, Gellibolian R, Shu Y, Dotimas H, Wang X-M, et al.: Homogeneous assays for cellular protein degradation using β-galactosidase complementation: NF-κB/IκB pathway signaling. Assay Drug Dev Technol 2003;6:823-833.
34. Naqvi T, Lim A, Rouhani R, Singh R, Eglen RM: β Galactosidase enzyme fragment complementation as a high throughput screening protease technology. J Biomol Screen 2004;9:398-408.
35. Liebig H-D, Skern T, Luderer M, Sommergruber W, Blaas D, Kuechler E: Proteinase trapping: screening for viral proteinase mutants by α complementation. Proc Natl Acad Sci U S A 1991;88:5979-5983.
36. O'Dowd BF, Alijaniaram M, Ji X, Nguyen T, Eglen RM, George SR: Utilizing ligand induced conformational change to screen for compounds targeting G protein coupled receptors. J Biomol Screen 2007 (in press).
37. Holland AU, Munk C, Lucero GR, Nguyen, LD, Landau NR: α Complementation assay for HIV envelope glycoprotein-mediated fusion. Virology 2003;319:343-352.
38. Mohler WA, Blau HM: Gene expression and cell fusion analyzed by lac Z complementation in mammalian cells. Proc Natl Acad Sci U S A 1996;93:12423-12427.
39. Rossi F, Charlton CA, Blau HM: Monitoring protein-protein interactions in intact eukaryotic cells by β-galactosidase complementation. Proc Natl Acad Sci U S A 1997;94:8405-8410.
40. Rossi FM, Blakely BT, Blau HM: Interaction blues: protein interactions monitored in live mammalian cells by β-galactosidase complementation. Trends Cell Biol 2000;10:119-122.
41. Blakely BT, Rossi FM, Tillotson B, Palmer M, Estelles A, Blau HM: Epidermal growth factor receptor dimerization monitored in live cells. Nat Biotechnol 2000;18:218-222.
42. Yan Y-X, Boldt-Houle DM, Tillotson BP, Gee MA, D'Eon BJ, Chang X-J, et al.: Cell-based high-throughput screening assay system for monitoring G protein-coupled receptor activation using β-galactosidase enzyme complementation technology. J Biomol Screen 2002;7:451-459.
43. Büttner FH, Kumpf R, Menzel S, Reulle D, Valler MJ: Evaluation of the InteraX system technology in a high-throughput screening environment. J Biomol Screen 2005;10:485-494.
44. Graham DL, Bevan N, Lowe PN, Palmer M, Rees S: Application of β-galactosidase enzyme complementation technology as a high throughput screening format for antagonists of the epidermal growth factor receptor. J Biomol Screen 2001;6:401-411.
45. Wehrman TS, Casipit CL, Gewertz NM, Blau HM: Enzymatic detection of protein translocation. Nat Methods 2005;2:521-527.
46. Fung P, Peng K, Kobel P, Dotimas H, Kauffman L, Olson K, et al.: A homogeneous cell-based assay to measure nuclear translocation using β galactosidase enzyme fragment complementation (β Gal EFC). Assay Drug Dev Technol 2006;4:263-272.