Pulse proteolysis: A simple method for quantitative determination of protein stability and ligand binding

Nature Methods

Volumn 2, Issue 3, 2005, Pages 207-212

  Park, Chiwook ^a   Marqusee, Susan ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI PROTEIN; GLUCOSE; LIGAND; MALTOSE; MALTOSE BINDING PROTEIN; NEW DRUG; RIBONUCLEASE H; SUCROSE; PEPTIDE HYDROLASE; RIBONUCLEASE HI; THERMOLYSIN;

ANALYTIC METHOD; ARTICLE; CELL LYSATE; CONTROLLED STUDY; ESCHERICHIA COLI; HIGH THROUGHPUT SCREENING; LIGAND BINDING; MONITORING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN VARIANT; QUANTITATIVE ANALYSIS; CHEMISTRY; COMPARATIVE STUDY; DRUG STABILITY; ENZYME ACTIVATION; ENZYME STABILITY; EVALUATION; FLOW INJECTION ANALYSIS; KINETICS; METHODOLOGY; PROTEIN BINDING; PROTEIN CONFORMATION;

ESCHERICHIA COLI;

DRUG STABILITY; ENZYME ACTIVATION; ENZYME STABILITY; FLOW INJECTION ANALYSIS; KINETICS; PEPTIDE HYDROLASES; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN INTERACTION MAPPING; RIBONUCLEASE H, CALF THYMUS; THERMOLYSIN;

EID: 18744391410     PISSN: 15487091     EISSN: None     Source Type: Journal    
DOI: 10.1038/nmeth740     Document Type: Article

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