메뉴 건너뛰기




Volumn 1160, Issue , 2014, Pages 43-53

mRNA and microRNA purity and integrity: The key to success in expression profiling

Author keywords

Denaturing gel electrophoresis; Microfluidic capillary electrophoresis; MicroRNA (miRNA); MIQE; mRNA; qPCR; RNA integrity; RNA purity; UV Vis spectrophotometer

Indexed keywords

GENOMIC DNA; MESSENGER RNA; MICRORNA; NUCLEASE;

EID: 84921424818     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-0733-5_5     Document Type: Article
Times cited : (11)

References (15)
  • 1
    • 77649229497 scopus 로고    scopus 로고
    • Riedmaier I et al (2010) mRNA and microRNA quality control for RT-qPCR analysis
    • Becker C, Hammerle-Fickinger A, Riedmaier I et al (2010) mRNA and microRNA quality control for RT-qPCR analysis. Methods 50:237–243. doi: 10.1016/j.ymeth.2010.01.010
    • Methods , vol.50 , pp. 237-243
    • Becker, C.1    Hammerle-Fickinger, A.2
  • 2
    • 33644975004 scopus 로고    scopus 로고
    • RNA integrity and the effect on the real-time qRT-PCR performance
    • Fleige S, Pfaffl MW (2006) RNA integrity and the effect on the real-time qRT-PCR performance. Mol Aspects Med 27:126–139
    • (2006) Mol Aspects Med , vol.27 , pp. 126-139
    • Fleige, S.1    Pfaffl, M.W.2
  • 3
    • 79955999748 scopus 로고    scopus 로고
    • Measurable impact of RNA quality on gene expression results from quantitative PCR
    • Vermeulen J, Prete K, de Lefever S (2011) Measurable impact of RNA quality on gene expression results from quantitative PCR. Nucleic Acids Res 39(9):e63. doi: 10.1093/nar/gkr065
    • (2011) Nucleic Acids Res , vol.39 , Issue.9 , pp. 63
    • Vermeulen, J.1    Prete, K.2    De Lefever, S.3
  • 4
    • 33847643776 scopus 로고    scopus 로고
    • Quantifi cation of mRNA using real-time RT-PCR
    • Nolan T, Hands RE, Bustin SA (2006) Quantifi cation of mRNA using real-time RT-PCR. Nat Protoc 1:1559–1582
    • (2006) Nat Protoc , vol.1 , pp. 1559-1582
    • Nolan, T.1    Hands, R.E.2    Bustin, S.A.3
  • 5
    • 24944584586 scopus 로고    scopus 로고
    • Impact of RNA quality on reference gene expression stability
    • Pérez-Novo CA, Claeys C, Speleman F et al (2005) Impact of RNA quality on reference gene expression stability. BioTechniques 39:54–56
    • (2005) Biotechniques , vol.39 , pp. 54-56
    • Pérez-Novo, C.A.1    Claeys, C.2    Speleman, F.3
  • 6
    • 0038012549 scopus 로고    scopus 로고
    • Biological sample collection and processing for molecular epidemiological studies
    • Holland NT, Smith MT, Eskenazi B et al (2003) Biological sample collection and processing for molecular epidemiological studies. Mutat Res 543:217–234
    • (2003) Mutat Res , vol.543 , pp. 217-234
    • Holland, N.T.1    Smith, M.T.2    Eskenazi, B.3
  • 7
    • 0345168051 scopus 로고    scopus 로고
    • Moderate degradation does not preclude microarray analysis of small amounts of RNA
    • Schoor O, Weinschenk T, Hennenlotter J et al (2003) Moderate degradation does not preclude microarray analysis of small amounts of RNA. BioTechniques 35(1192–1196):1198–1201
    • (2003) Biotechniques , vol.35 , Issue.1192-1196 , pp. 1198-1201
    • Schoor, O.1    Weinschenk, T.2    Hennenlotter, J.3
  • 8
    • 64149097786 scopus 로고    scopus 로고
    • The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments
    • Bustin SA, Benes V, Garson JA et al (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55:611–622
    • (2009) Clin Chem , vol.55 , pp. 611-622
    • Bustin, S.A.1    Benes, V.2    Garson, J.A.3
  • 9
    • 33644985661 scopus 로고    scopus 로고
    • Nucleic acids: MRNA identifi cation and quantifi cation
    • In: Worsfold P, Townshend A, Poole CF, 2nd edn. Elsevier Academic Press, Amsterdam, Boston
    • Pfaffl MW (2005) Nucleic acids: mRNA identifi cation and quantifi cation. In: Worsfold P, Townshend A, Poole CF (eds) Encyclopedia of analytical science, 2nd edn. Elsevier Academic Press, Amsterdam, Boston, pp 417–426
    • (2005) Encyclopedia of Analytical Science , pp. 417-426
    • Pfaffl, M.W.1
  • 10
    • 0030004766 scopus 로고    scopus 로고
    • Use of UV methods for measurement of protein and nucleic acid concentrations
    • Manchester KL (1996) Use of UV methods for measurement of protein and nucleic acid concentrations. BioTechniques 20:968–970
    • (1996) Biotechniques , vol.20 , pp. 968-970
    • Manchester, K.L.1
  • 11
    • 33645293441 scopus 로고    scopus 로고
    • SPUD: A quantitative PCR assay for the detection of inhibitors in nucleic acid preparations
    • Nolan T, Hands RE, Ogunkolade W et al (2006) SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations. Anal Biochem 351:308–310
    • (2006) Anal Biochem , vol.351 , pp. 308-310
    • Nolan, T.1    Hands, R.E.2    Ogunkolade, W.3
  • 12
    • 33748095919 scopus 로고    scopus 로고
    • Comparison of relative mRNA quantifi cation models and the impact of RNA integrity in quantitative real-time RT-PCR
    • Fleige S, Walf V, Huch S et al (2006) Comparison of relative mRNA quantifi cation models and the impact of RNA integrity in quantitative real-time RT-PCR. Biotechnol Lett 28:1601–1613
    • (2006) Biotechnol Lett , vol.28 , pp. 1601-1613
    • Fleige, S.1    Walf, V.2    Huch, S.3
  • 13
    • 33645241407 scopus 로고    scopus 로고
    • The RIN: An RNA integrity number for assigning integrity values to RNA measurements
    • Mueller O, Lightfoot S, Schroeder A et al (2006) The RIN: an RNA integrity number for assigning integrity values to RNA measurements. BMC Mol Biol 7:3. doi: 10.1186/1471-2199-7-3
    • (2006) BMC Mol Biol , vol.7 , pp. 3
    • Mueller, O.1    Lightfoot, S.2    Schroeder, A.3
  • 14
    • 1842474964 scopus 로고    scopus 로고
    • Evaluating RNA status for RT-PCR in extracts of postmortem human brain tissue
    • Miller CL, Diglisic S, Leister F et al (2004) Evaluating RNA status for RT-PCR in extracts of postmortem human brain tissue. BioTechniques 36:628–633
    • (2004) Biotechniques , vol.36 , pp. 628-633
    • Miller, C.L.1    Diglisic, S.2    Leister, F.3
  • 15
    • 0023047211 scopus 로고
    • The relationship between mRNA stability and length in Saccharomyces cerevisiae
    • Santiago TC, Purvis IJ, Bettany AJ et al (1986) The relationship between mRNA stability and length in Saccharomyces cerevisiae. Nucleic Acids Res 14:8347–8360
    • (1986) Nucleic Acids Res , vol.14 , pp. 8347-8360
    • Santiago, T.C.1    Purvis, I.J.2    Bettany, A.J.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.