From deep sequencing to actual clones

Protein Engineering, Design and Selection

Volumn 27, Issue 10, 2014, Pages 301-307

  D'Angelo, Sara ^a   Kumar, Sandeep ^b,c   Naranjo, Leslie ^b   Ferrara, Fortunato ^a   Kiss, Csaba ^b   Bradbury, Andrew R M ^b   Daggett, Valerie ^d  

^a NEW MEXICO CONSORTIUM   (United States)

^b LOS ALAMOS NATIONAL LABORATORY   (United States)

^c Compugen USA Inc ^*  (United States)

^d NONE

Author keywords

Antibody; Deep sequencing; Inverse PCR; Phage display; Yeast display

Indexed keywords

ANTIBODIES; CLONE CELLS; DNA SEQUENCES; GENE ENCODING; INVERSE PROBLEMS; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; SCAFFOLDS (BIOLOGY); YEAST;

ANTIBODY GENES; DEEP SEQUENCING; DISPLAY TECHNOLOGIES; INVERSE PCR; MULTISTEP PROCESS; SCAFFOLD PROTEIN; UNIQUE SEQUENCE; YEAST DISPLAY;

CLONING;

CYCLIN DEPENDENT KINASE 2; PLASMID DNA; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; PEPTIDE LIBRARY;

AMINO ACID SEQUENCE; BACTERIAL CELL; BACTERIOPHAGE; CONFERENCE PAPER; DNA SEQUENCE; ENZYME INACTIVATION; EXPRESSION VECTOR; FLOW CYTOMETRY; IN VITRO STUDY; INVERSE POLYMERASE CHAIN REACTION; MOLECULAR CLONING; NONHUMAN; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SEQUENCE ANALYSIS; YEAST CELL; CELL SURFACE DISPLAY; GENETICS; HIGH THROUGHPUT SEQUENCING; LACTOBACILLUS ACIDOPHILUS; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PEPTIDE LIBRARY; PROCEDURES; YEAST;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELL SURFACE DISPLAY TECHNIQUES; CLONING, MOLECULAR; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; LACTOBACILLUS ACIDOPHILUS; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SINGLE-CHAIN ANTIBODIES; YEASTS;

EID: 84921328944     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzu032     Document Type: Conference Paper

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