Cloning, Overexpression, and Characterization of a Highly Active Endoinulinase Gene from Aspergillus fumigatus Cl1 for Production of Inulo-Oligosaccharides

Applied Biochemistry and Biotechnology

Volumn 175, Issue 2, 2015, Pages 1153-1167

  Chen, Min ^a   Lei, Xiao ^a   Chen, Chuze ^a   Zhang, Shiyang ^a   Xie, Jingli ^a   Wei, Dongzhi ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Aspergillus fumigatus; Endoinulinase; Immobilization; Overexpression; Pichia pastoris

Indexed keywords

CLONE CELLS; CLONING; GENES; LIQUID CHROMATOGRAPHY; OLIGOSACCHARIDES; RADIOACTIVE WASTE VITRIFICATION; THIN LAYER CHROMATOGRAPHY; YEAST;

ASPERGILLUS FUMIGATUS; DEGREE OF POLYMERIZATION; ENDOINULINASE; HIGH CELL DENSITY FERMENTATIONS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHS; OVER-EXPRESSION; PICHIA PASTORIS; RESIDUAL ACTIVITY;

ASPERGILLUS;

INULIN; OLIGOSACCHARIDE; GLYCOSIDASE; IMMOBILIZED ENZYME; METAL; RECOMBINANT PROTEIN;

ARTICLE; ASPERGILLUS FUMIGATUS; BIOTRANSFORMATION; CELL DENSITY; ENZYME PURIFICATION; GENE OVEREXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KOMAGATAELLA PASTORIS; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PH; SCALE UP; TEMPERATURE; THIN LAYER CHROMATOGRAPHY; AMINO ACID SEQUENCE; BIOREACTOR; BIOSYNTHESIS; CELL COUNT; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; FERMENTATION; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; PICHIA; PROTEIN CONFORMATION; SEQUENCE ANALYSIS;

ASPERGILLUS FUMIGATUS; PICHIA PASTORIS;

AMINO ACID SEQUENCE; ASPERGILLUS FUMIGATUS; BIOREACTORS; CELL COUNT; CLONING, MOLECULAR; ENZYMES, IMMOBILIZED; FERMENTATION; GENE EXPRESSION; GLYCOSIDE HYDROLASES; HYDROGEN-ION CONCENTRATION; INULIN; METALS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; PICHIA; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS; TEMPERATURE;

EID: 84921051974     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-014-1296-1     Document Type: Article

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