First crystal structure of an endo-inulinase, INU2, from Aspergillus ficuum: Discovery of an extra-pocket in the catalytic domain responsible for its endo-activity

Biochimie

Volumn 94, Issue 11, 2012, Pages 2423-2430

  Pouyez, Jenny ^a   Mayard, Aurélie ^a   Vandamme, Anne Michèle ^a   Roussel, Guillaume ^a   Perpète, Eric A ^a   Wouters, Johan ^a   Housen, Isabelle ^a   Michaux, Catherine ^a  

^a UNIVERSITY OF NAMUR   (Belgium)

Author keywords

Crystal structure; Endo inulinase; Enzyme mechanism; Glycoside hydrolase; Molecular modelling

Indexed keywords

ENDO INULINASE INU2; EXO INULINASE; FRUCTOSE; GLYCOSIDASE; GLYCOSIDASE HYDROLASE FAMILY 32; HYDROLASE; INULINASE; TRYPTOPHAN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ARTICLE; ASPERGILLUS FICUUM; BETA SHEET; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; COMPLEX FORMATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME METABOLISM; ENZYME SUBSTRATE; MOLECULAR BIOLOGY; MOLECULAR MODEL; MOLECULE; NONHUMAN; PROTEIN DOMAIN; PROTEIN FOLDING;

AMINO ACID SEQUENCE; ASPERGILLUS; BIOCATALYSIS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; GLYCOSIDE HYDROLASES; KINETICS; MOLECULAR DOCKING SIMULATION; MOLECULAR SEQUENCE DATA;

ASPERGILLUS FICUUM;

EID: 84867404044     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2012.06.020     Document Type: Article

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