Expression of the INU2 gene for an endoinulinase of Aspergillus ficuum in Saccharomyces cerevisiae

Biotechnology Letters

Volumn 21, Issue 7, 1999, Pages 621-623

  Kim, Hee Seo ^a   Lee, Dong Whan ^a   Ryu, Eun Ja ^a   Uhm, Tai Boong ^a   Yang, Moon Sik ^a   Kim, Jung Bae ^b   Chae, Keon Sang ^a  

^a Chonbuk National University   (South Korea)

^b R and D Center   (South Korea)

Author keywords

Aspergillus ficuum; Endoinulinase; Gene: INU2; Kluyveromyces marxianus; Overexpression; Saccharomyces cerevisiae

Indexed keywords

BETA FRUCTOFURANOSIDASE; INULINASE;

ARTICLE; ASPERGILLUS; COLONY FORMATION; CULTURE MEDIUM; EXPRESSION VECTOR; GENETIC RECOMBINATION; NONHUMAN; PROMOTER REGION; SACCHAROMYCES CEREVISIAE;

ASPERGILLUS FICUUM; SACCHAROMYCES CEREVISIAE;

EID: 0032866243     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005567403830     Document Type: Article

References (9)

1. Bergkamp RJ, Bootsman TC, Toschka HY, Mooren ATA, Kox L, Verbakel JMA, Geerse RH, Planta RJ (1993) Expression of an alpha-galactosidase gene under control of the homologous inulinase promoter in Kluyveromyces marxianus. Appl. Microbiol. Biotechnol. 40: 309-317.
2. Ito H, Fukuda Y, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153: 163-168.
3. Luscher M, Erdin C, Sprenger N, Hochstrasser U, Boller T, Wiemken A (1996) Inulin synthesis by a combination of purified fructosyltransferases from tubers of Helianthus tuberosus. FEBS Lett. 385: 39-42.
4. Nakamura T, Maruki S, Nakatsu S, Ueda S (1978) General properties of an extracellular inulinase (P-II) from Aspergillus sp. Nippon Nogei Kagaku Kaishi 52: 581-587 (in Japanese).
5. Rouwenhorst RJ, Hensing M, Verbakel J, Scheffers WA, van Duken JP (1990) Structure and properties of the extracellular inulinase of Kluyveromyces marxianus CBS 6556. Appl. Environ. Microbiol. 56: 3337-3345.
6. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor.
7. Sarokin L, Carlson M (1985) Upstream region required for regulated expression of the glucose-repressible SUC2 gene of Saccharomyces cerevisiae. Mol. Cell. Biol. 5: 2521-2526.
8. Uhm TB, Chae K-S, Lee DW, Kim H-S, Cassart J-P, Vandenhaute J (1998) Cloning and nucleotide sequence of the endoinulinase-encoding gene, inu2, from Aspergillus ficuum. Biotechnol. Lett. 20: 809-812.
9. Zittan L (1981) Enzymatic hydrolysis of inulin-an alternative way to fructose production. Starch 33: 373-377.