Precise construction of oligonucleotide-Fab fragment conjugate for homogeneous immunoassay using HaloTag technology

Analytical Biochemistry

Volumn 472, Issue , 2015, Pages 37-44

  Päkkilä, Henna ^a   Peltomaa, Riikka ^a   Lamminmäki, Urpo ^a   Soukka, Tero ^a  

^a UNIVERSITY OF TURKU   (Finland)

Author keywords

Bioconjugation; Homogeneous detection; Immunoassay; Lanthanide chelate

Indexed keywords

ANTIGENS; IMMUNOLOGY; PROTEINS; PURIFICATION; RARE EARTH ELEMENTS;

BIO-CONJUGATION; BIOANALYTICAL ASSAYS; DNA OLIGONUCLEOTIDES; HOMOGENEOUS DETECTIONS; IMMUNOASSAY; LANTHANIDE CHELATES; LANTHANIDE LUMINESCENCE; PROSTATE SPECIFIC ANTIGEN;

OLIGONUCLEOTIDES;

HEXAHISTIDINE; HYBRID PROTEIN; IMMUNOGLOBULIN F(AB) FRAGMENT; LANTHANIDE; LIGAND; NITRILOTRIACETATE NICKEL; OLIGONUCLEOTIDE; PROTEIN G; BACTERIAL PROTEIN; IGG FC-BINDING PROTEIN, STREPTOCOCCUS; KALLIKREIN; KALLIKREIN-RELATED PEPTIDASE 3, HUMAN; OLIGODEOXYRIBONUCLEOTIDE; PROSTATE SPECIFIC ANTIGEN;

ARTICLE; CHEMOLUMINESCENCE; CONJUGATE; CONJUGATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOASSAY; LIMIT OF DETECTION; MEDICAL TECHNOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; ANIMAL; CHEMISTRY; HUMAN; PROCEDURES; RABBIT;

ANIMALS; BACTERIAL PROTEINS; HUMANS; IMMUNOASSAY; IMMUNOGLOBULIN FAB FRAGMENTS; KALLIKREINS; OLIGODEOXYRIBONUCLEOTIDES; PROSTATE-SPECIFIC ANTIGEN; RABBITS;

EID: 84921038433     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.11.018     Document Type: Article

References (45)

1. S.B. Rajur, C.M. Roth, J.R. Morgan, M.L. Yarmush, Covalent protein-oligonucleotide conjugates for efficient delivery of antisense molecules, Bioconjug. Chem. 8 (1997) 935-940.
2. T.P. Wang, N.C. Ko, Y.C. Su, E.C. Wang, S. Severance, C.C. Hwang, Y.T. Shih, M.H. Wu, Y.H. Chen, Advanced aqueous-phase phosphoramidation reactions for effectively synthesizing peptide-oligonucleotide conjugates trafficked into a human cell line, Bioconjug. Chem. 23 (2012) 2417-2433.
3. K.J. Leuchowius, I. Weibrecht, U. Landegren, L. Gedda, O. Soderberg, Flow cytometric in situ proximity ligation analyses of protein interactions and post-translational modification of the epidermal growth factor receptor family, Cytometry A 75 (2009) 833-839.
4. G.J. Gu, M. Friedman, C. Jost, K. Johnsson, M. Kamali-Moghaddam, A. Plückthun, U. Landegren, O. Soderberg, Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation, New Biotechnol. 30 (2013) 144-152.
5. S. Gandor, S. Reisewitz, M. Venkatachalapathy, G. Arrabito, M. Reibner, H. Schroder, K. Ruf, C.M. Niemeyer, P.I. Bastiaens, L. Dehmelt, A protein-interaction array inside a living cell, Angew. Chem. Int. Ed. Engl. 52 (2013) 4790-4794.
6. R. Wacker, H. Schroder, C.M. Niemeyer, Performance of antibody microarrays fabricated by either DNA-directed immobilization, direct spotting, or streptavidin-biotin attachment: a comparative study, Anal. Biochem. 330 (2004) 281-287.
7. C. Boozer, J. Ladd, S. Chen, S. Jiang, DNA-directed protein immobilization for simultaneous detection of multiple analytes by surface plasmon resonance biosensor, Anal. Chem. 78 (2006) 1515-1519.
8. A.L. Washburn, J. Gomez, R.C. Bailey, DNA-encoding to improve performance and allow parallel evaluation of the binding characteristics of multiple antibodies in a surface-bound immunoassay format, Anal. Chem. 83 (2011) 3572-3580.
9. T. Sano, C.L. Smith, C.R. Cantor, Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates, Science 258 (1992) 120-122.
10. J.E. McDermed, R. Sanders, S. Fait, R.E. Klem, M.J. Sarno, T.H. Adams, E.P. Diamandis, Nucleic acid detection immunoassay for prostate-specific antigen based on immuno-PCR methodology, Clin. Chem. 58 (2012) 732-740.
11. B. Schweitzer, S. Wiltshire, J. Lambert, S. O'Malley, K. Kukanskis, Z. Zhu, S.F. Kingsmore, P.M. Lizardi, D.C. Ward, Immunoassays with rolling circle DNA amplification: a versatile platform for ultrasensitive antigen detection, Proc. Natl. Acad. Sci. U.S.A. 97 (2000) 10113-10119.
12. M. Gullberg, S.M. Gustafsdottir, E. Schallmeiner, J. Jarvius, M. Bjarnegard, C. Betsholtz, U. Landegren, S. Fredriksson, Cytokine detection by antibody-based proximity ligation, Proc. Natl. Acad. Sci. U.S.A. 101 (2004) 8420-8424.
13. E. Heyduk, B. Dummit, Y.H. Chang, T. Heyduk, Molecular pincers: antibody-based homogeneous protein sensors, Anal. Chem. 80 (2008) 5152-5159.
14. E. Heyduk, M.M. Moxley, A. Salvatori, J.A. Corbett, T. Heyduk, Homogeneous insulin and C-peptide sensors for rapid assessment of insulin and C-peptide secretion by the islets, Diabetes 59 (2010) 2360-2365.
15. H. Päkkilä, S. Blom, K. Kopra, T. Soukka, Aptamer-directed lanthanide chelate self-assembly for rapid thrombin detection, Analyst 138 (2013) 5107-5112.
16. V. Santala, U. Lamminmäki, Production of a biotinylated single-chain antibody fragment in the cytoplasm of Escherichia coli, J. Immunol. Methods 284 (2004) 165-175.
17. J. Ylikotila, L. Välimaa, M. Vehniäinen, H. Takalo, T. Lövgren, K. Pettersson, A sensitive TSH assay in spot-coated microwells utilizing recombinant antibody fragments, J. Immunol. Methods 306 (2005) 104-114.
18. D.M. Bauer, I. Ahmed, A. Vigovskaya, L. Fruk, Clickable tyrosine binding bifunctional linkers for preparation of DNA-protein conjugates, Bioconjug. Chem. 24 (2013) 1094-1101.
19. T. Sano, S. Vajda, G.O. Reznik, C.L. Smith, C.R. Cantor, Molecular engineering of streptavidin, Ann. N. Y. Acad. Sci. 799 (1996) 383-390.
20. M. Howarth, D.J. Chinnapen, K. Gerrow, P.C. Dorrestein, M.R. Grandy, N.L. Kelleher, A. El-Husseini, A.Y. Ting, A monovalent streptavidin with a single femtomolar biotin binding site, Nat. Methods 3 (2006) 267-273.
21. B. Saccá, R. Meyer, M. Erkelenz, K. Kiko, A. Arndt, H. Schroeder, K.S. Rabe, C.M. Niemeyer, Orthogonal protein decoration of DNA origami, Angew. Chem. Int. Ed. Engl. 49 (2010) 9378-9383.
22. G.V. Los, L.P. Encell, M.G. McDougall, D.D. Hartzell, N. Karassina, C. Zimprich, M.G. Wood, R. Learish, R.F. Ohana, M. Urh, D. Simpson, J. Mendez, K. Zimmerman, P. Otto, G. Vidugiris, J. Zhu, A. Darzins, D.H. Klaubert, R.F. Bulleit, K.V. Wood, HaloTag: a novel protein labeling technology for cell imaging and protein analysis, ACS Chem. Biol. 3 (2008) 373-382.
23. A. Keppler, S. Gendreizig, T. Gronemeyer, H. Pick, H. Vogel, K. Johnsson, A general method for the covalent labeling of fusion proteins with small molecules in vivo, Nat. Biotechnol. 21 (2003) 86-89.
24. A. Gautier, A. Juillerat, C. Heinis, I.R. Correa Jr., M. Kindermann, F. Beaufils, K. Johnsson, An engineered protein tag for multiprotein labeling in living cells, Chem. Biol. 15 (2008) 128-136.
25. N. Nath, R. Hurst, B. Hook, P. Meisenheimer, K.Q. Zhao, N. Nassif, R.F. Bulleit, D.R. Storts, Improving protein array performance: focus on washing and storage conditions, J. Proteome Res. 7 (2008) 4475-4482.
26. D.J. Noblin, C.M. Page, H. Tae, P.C. Gareiss, J.S. Schneekloth, C.M. Crews, A HaloTag-based small molecule microarray screening methodology with increased sensitivity and multiplex capabilities, ACS Chem. Biol. 7 (2012) 2055-2063.
27. H. Hong, H.A. Benink, Y. Zhang, Y. Yang, H.T. Uyeda, J.W. Engle, G.W. Severin, M.G. McDougall, T.E. Barnhart, D.H. Klaubert, R.J. Nickles, F. Fan, W. Cai, HaloTag: a novel reporter gene for positron emission tomography, Am. J. Transl. Res. 3 (2011) 392-403.
28. N. Kosaka, M. Ogawa, P.L. Choyke, N. Karassina, C. Corona, M. McDougall, D.T. Lynch, C.C. Hoyt, R.M. Levenson, G.V. Los, H. Kobayashi, In vivo stable tumor-specific painting in various colors using dehalogenase-based protein-tag fluorescent ligands, Bioconjug. Chem. 20 (2009) 1367-1374.
29. T. Hata, M. Nakayama, Rapid single-tube method for small-scale affinity purification of polyclonal antibodies using HaloTag technology, J. Biochem. Biophys. Methods 70 (2007) 679-682.
30. R. Hurst, B. Hook, M.R. Slater, J. Hartnett, D.R. Storts, N. Nath, Protein-protein interaction studies on protein arrays: effect of detection strategies on signal-to-background ratios, Anal. Biochem. 392 (2009) 45-53.
31. D.D. Hartzell, N.D. Trinklein, J. Mendez, N. Murphy, S.F. Aldred, K. Wood, M. Urh, A functional analysis of the CREB signaling pathway using HaloCHIP-chip and high throughput reporter assays, BMC Genomics 10 (2009) 497.
32. M. Erkelenz, C.H. Kuo, C.M. Niemeyer, DNA-mediated assembly of cytochrome P450 BM3 subdomains, J. Am. Chem. Soc. 133 (2011) 16111-16118.
33. K. Rajakoski, T. Piironen, K. Pettersson, J. Lövgren, M. Karp, Epitope mapping of human prostate specific antigen and glandular kallikrein expressed in insect cells, Prostate Cancer Prostatic Dis. 1 (1997) 16-20.
34. T. Piironen, B.O. Villoutreix, C. Becker, K. Hollingsworth, M. Vihinen, D. Bridon, X. Qiu, J. Rapp, B. Dowell, T. Lövgren, K. Pettersson, H. Lilja, Determination and analysis of antigenic epitopes of prostate specific antigen (PSA) and human glandular kallikrein 2 (hK2) using synthetic peptides and computer modeling, Protein Sci. 7 (1998) 259-269.
35. Q. Qin, M. Christiansen, T. Lövgren, B. Nørgaard-Pedersen, K. Pettersson, Dual-labeled time-resolved immunofluorometric assay for simultaneous determination of pregnancy-associated plasma protein A and free b-subunit of human chorionic gonadotrophin, J. Immunol. Methods 205 (1997) 169-175.
36. U. Karhunen, L. Jaakkola, Q. Wang, U. Lamminmäki, T. Soukka, Luminescence switching by hybridization-directed mixed lanthanide complex formation, Anal. Chem. 82 (2010) 751-754.
37. U. Karhunen, J. Rosenberg, U. Lamminmäki, T. Soukka, Homogeneous detection of avidin based on switchable lanthanide luminescence, Anal. Chem. 83 (2011) 9011-9016.
38. S. Eriksson, M. Vehniäinen, T. Jansen, V. Meretoja, P. Saviranta, K. Pettersson, T. Lövgren, Dual-label time-resolved immunofluorometric assay of free and total prostate-specific antigen based on recombinant Fab fragments, Clin. Chem. 46 (2000) 658-666.
39. A. Krebber, S. Bornhauser, J. Burmester, A. Honegger, J. Willuda, H.R. Bosshard, A. Plückthun, Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system, J. Immunol. Methods 201 (1997) 35-55.
40. T. Korpimäki, V. Hagren, E.C. Brockmann, M. Tuomola, Generic lanthanide fluoroimmunoassay for the simultaneous screening of 18 sulfonamides using an engineered antibody, Anal. Chem. 76 (2004) 3091-3098.
41. I. Hemmilä, Fluoroimmunoassays and immunofluorometric assays, Clin. Chem. 31 (1985) 359-370.
42. T. Kokko, T. Liljenbäck, M.T. Peltola, L. Kokko, T. Soukka, Homogeneous dual-parameter assay for prostate-specific antigen based on fluorescence resonance energy transfer, Anal. Chem. 80 (2008) 9763-9768.
43. A. Valanne, H. Lindroos, T. Lövgren, T. Soukka, A novel homogeneous assay format utilising proximity dependent fluorescence energy transfer between particulate labels, Anal. Chim. Acta 539 (2005) 251-256.
44. A. Heidenreich, P.J. Bastian, M. Bolla, T. van der Kwast, M. Mason, V. Matveev, T. Wiegel, F. Zattoni, N. Mottet, European Association of Urology, EAU guidelines on prostate cancer: 1. Screening, diagnosis, and local treatment with curative intent - Update, 2013, Eur. Urol. 65 (2014) 124-137.
45. H. Päkkilä, E. Malmi, S. Lahtinen, T. Soukka, Rapid homogeneous immunoassay for cardiac troponin I using switchable lanthanide luminescence, Biosens. Bioelectron. 62 (2014) 201-207.