Halotag: A novel reporter gene for positron emission tomography

American Journal of Translational Research

Volumn 3, Issue 4, 2011, Pages 392-403

  Hong, Hao ^a   Benink, Hélène A ^b   Zhang, Yin ^c   Yang, Yunan ^a   Uyeda, H Tetsuo ^d   Engle, Jonathan W ^c   Severin, Gregory W ^c   McDougall, Mark G ^d   Barnhart, Todd E ^c   Klaubert, Dieter H ^d   Nickles, Robert J ^c   Fan, Frank ^b   Cai, Weibo ^a,c,e  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b PROMEGA CORPORATION   (United States)

^c UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

^d Promega Biosciences LLC   (United States)

^e UNIVERSITY OF WISCONSIN   (United States)

Author keywords

Cancer; Cell tracking; Halotag; Molecular imaging; Positron emission tomography (PET); Reporter gene

Indexed keywords

1,4,7 TRIAZACYCLONONANE N,N',N'' TRIACETIC ACID HALOTAG CU 64; ALKYL CHLORIDE; HALOALKANE DEHALOGENASE; HYDROLASE; RADIOPHARMACEUTICAL AGENT; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BREAST CANCER; CANCER CELL CULTURE; DRUG DISTRIBUTION; FEMALE; HALOTAG GENE; IN VITRO STUDY; IN VIVO STUDY; MOUSE; NONHUMAN; POSITRON EMISSION TOMOGRAPHY; REPORTER GENE;

MUS;

EID: 80051729814     PISSN: None     EISSN: 19438141     Source Type: Journal    
DOI: None     Document Type: Article

References (49)

1. Massoud TF and Gambhir SS. Molecular imag-ing in living subjects: seeing fundamental bio-logical processes in a new light. Genes Dev 2003; 17: 545-580.
2. Weissleder R and Pittet MJ. Imaging in the era of molecular oncology. Nature 2008; 452: 580-589.
3. Kang JH and Chung JK. Molecular-genetic imag-ing based on reporter gene expression. J Nucl Med 2008; 49 Suppl 2: 164S-179S.
4. Jacobs A, Voges J, Reszka R, Lercher M, Gossmann A, Kracht L, Kaestle C, Wagner R, Wienhard K and Heiss WD. Positron-emission tomography of vector-mediated gene expres-sion in gene therapy for gliomas. Lancet 2001; 358: 727-729.
5. Yaghoubi SS, Jensen MC, Satyamurthy N, Budhiraja S, Paik D, Czernin J and Gambhir SS. Noninvasive detection of therapeutic cytolytic T cells with 18F-FHBG PET in a patient with glioma. Nat Clin Pract Oncol 2009; 6: 53-58.
6. Giepmans BN, Adams SR, Ellisman MH and Tsien RY. The fluorescent toolbox for assessing protein location and function. Science 2006; 312: 217-224.
7. Contag CH and Bachmann MH. Advances in in vivo bioluminescence imaging of gene expres-sion. Annu Rev Biomed Eng 2002; 4: 235-260.
8. Herschman HR. Noninvasive imaging of re-porter gene expression in living subjects. Adv Cancer Res 2004; 92: 29-80.
9. Min JJ and Gambhir SS. Molecular imaging of PET reporter gene expression. Handb Exp Phar-macol 2008; 277-303.
10. Gilad AA, Ziv K, McMahon MT, van Zijl PC, Nee-man M and Bulte JW. MRI reporter genes. J Nucl Med 2008; 49: 1905-1908.
11. Los GV and Wood K. The HaloTag: a novel tech-nology for cell imaging and protein analysis. Methods Mol Biol 2007; 356: 195-208.
12. Los GV, Encell LP, McDougall MG, Hartzell DD, Karassina N, Zimprich C, Wood MG, Learish R, Ohana RF, Urh M, Simpson D, Mendez J, Zim-merman K, Otto P, Vidugiris G, Zhu J, Darzins A, Klaubert DH, Bulleit RF and Wood KV. HaloTag: a novel protein labeling technology for cell im-aging and protein analysis. ACS Chem Biol 2008; 3: 373-382.
13. Zhang Y, So MK, Loening AM, Yao H, Gambhir SS and Rao J. HaloTag protein-mediated site-specific conjugation of bioluminescent proteins to quantum dots. Angew Chem Int Ed Engl 2006; 45: 4936-4940.
14. Lang C, Schulze J, Mendel RR and Hansch R. HaloTag: a new versatile reporter gene system in plant cells. J Exp Bot 2006; 57: 2985-2992.
15. So MK, Yao H and Rao J. HaloTag protein-mediated specific labeling of living cells with quantum dots. Biochem Biophys Res Commun 2008; 374: 419-423.
16. Takemoto K, Matsuda T, McDougall M, Klaubert DH, Hasegawa A, Los GV, Wood KV, Miyawaki A and Nagai T. Chromophore-assisted light inactivation of HaloTag fusion proteins labeled with eosin in living cells. ACS Chem Biol 2011; 6: 401-406.
17. Hata T and Nakayama M. Rapid single-tube method for small-scale affinity purification of polyclonal antibodies using HaloTag Technol-ogy. J Biochem Biophys Methods 2007; 70: 679-682.
18. Motejadded H, Kranz B, Berensmeier S, Franzreb M and Altenbuchner J. Expression, one-step purification, and immobilization of HaloTag(TM) fusion proteins on chloroalkane-functionalized magnetic beads. Appl Biochem Biotechnol 2010; 162: 2098-2110.
19. Chumanov RS, Kuhn PA, Xu W and Burgess RR. Expression and purification of full-length mouse CARM1 from transiently transfected HEK293T cells using HaloTag technology. Protein Expr Purif 2011; 76: 145-153.
20. Ohana RF, Hurst R, Vidugiriene J, Slater MR, Wood KV and Urh M. HaloTag-based purifica-tion of functional human kinases from mam-malian cells. Protein Expr Purif 2011; 76: 154-164.
21. Svendsen S, Zimprich C, McDougall MG, Klaubert DH and Los GV. Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter. BMC Cell Biol 2008; 9: 17.
22. Urh M, Hartzell D, Mendez J, Klaubert DH and Wood K. Methods for detection of protein-protein and protein-DNA interactions using HaloTag. Methods Mol Biol 2008; 421: 191-209.
23. Schroder J, Benink H, Dyba M and Los GV. In vivo labeling method using a genetic construct for nanoscale resolution microscopy. Biophys J 2009; 96: L01-03.
24. Kosaka N, Ogawa M, Choyke PL, Karassina N, Corona C, McDougall M, Lynch DT, Hoyt CC, Levenson RM, Los GV and Kobayashi H. In vivo stable tumor-specific painting in various colors using dehalogenase-based protein-tag fluores-cent ligands. Bioconjug Chem 2009; 20: 1367-1374.
25. Yamaguchi K, Inoue S, Ohara O and Nagase T. Pulse-chase experiment for the analysis of pro-tein stability in cultured mammalian cells by covalent fluorescent labeling of fusion proteins. Methods Mol Biol 2009; 577: 121-131.
26. Hartzell DD, Trinklein ND, Mendez J, Murphy N, Aldred SF, Wood K and Urh M. A functional analysis of the CREB signaling pathway using HaloCHIP-chip and high throughput reporter assays. BMC Genomics 2009; 10: 497.
27. Taniguchi Y and Kawakami M. Application of HaloTag protein to covalent immobilization of recombinant proteins for single molecule force spectroscopy. Langmuir 2010; 26: 10433-10436.
28. Gallo S, Beugnet A and Biffo S. Tagging of func-tional ribosomes in living cells by HaloTag(R) technology. In Vitro Cell Dev Biol Anim 2011; 47: 132-138.
29. Zhou ZP, Shimizu Y, Tadakuma H, Taguchi H, Ito K and Ueda T. Single molecule imaging of the trans-translation entry process via anchor-ing of the tagged ribosome. J Biochem 2011; 149: 609-618.
30. Wang H, Cai W, Chen K, Li ZB, Kashefi A, He L and Chen X. A new PET tracer specific for vas-cular endothelial growth factor receptor 2. Eur J Nucl Med Mol Imaging 2007; 34: 2001-2010.
31. Hong H, Yang Y, Zhang Y, Engle JW, Barnhart TE, Nickles RJ, Leigh BR and Cai W. Positron emission tomography imaging of CD105 ex-pression during tumor angiogenesis. Eur J Nucl Med Mol Imaging 2011; 38: 1335-1343.
32. Cai W, Zhang X, Wu Y and Chen X. A thiol-reactive 18F-labeling agent, N-[2-(4-18F-fluorobenzamido)ethyl]maleimide (18F-FBEM), and the synthesis of RGD peptide-based tracer for PET imaging of avb3 integrin expression. J Nucl Med 2006; 47: 1172-1180.
33. Zhang X, Cai W, Cao F, Schreibmann E, Wu Y, Wu JC, Xing L and Chen X. 18F-labeled bombe-sin analogs for targeting GRP receptor-expressing prostate cancer. J Nucl Med 2006; 47: 492-501.
34. Wadas TJ, Wong EH, Weisman GR and Ander-son CJ. Coordinating radiometals of copper, gallium, indium, yttrium, and zirconium for PET and SPECT imaging of disease. Chem Rev 110: 2858-2902.
35. Dearling JL, Voss SD, Dunning P, Snay E, Fahey F, Smith SV, Huston JS, Meares CF, Treves ST and Packard AB. Imaging cancer using PET--the effect of the bifunctional chelator on the biodis-tribution of a 64Cu-labeled antibody. Nucl Med Biol 2011; 38: 29-38.
36. Cai W, Chen K, He L, Cao Q, Koong A and Chen X. Quantitative PET of EGFR expression in xeno-graft-bearing mice using 64Cu-labeled cetuxi-mab, a chimeric anti-EGFR monoclonal anti-body. Eur J Nucl Med Mol Imaging 2007; 34: 850-858.
37. Cai W, Ebrahimnejad A, Chen K, Cao Q, Li ZB, Tice DA and Chen X. Quantitative radioim-munoPET imaging of EphA2 in tumour-bearing mice. Eur J Nucl Med Mol Imaging 2007; 34: 2024-2036.
38. Cai W, Wu Y, Chen K, Cao Q, Tice DA and Chen X. In vitro and in vivo characterization of 64Cu-labeled AbegrinTM, a humanized monoclonal antibody against integrin avb3. Cancer Res 2006; 66: 9673-9681.
39. Mammen M, Chio S and Whitesides GM. Poly-valent interactions in biological systems: impli-cations for design and use of multivalent ligands and inhibitors. Angew Chem Int Ed Engl 1998; 37: 2755-2794.
40. Cai W, Niu G and Chen X. Imaging of integrins as biomarkers for tumor angiogenesis. Curr Pharm Des 2008; 14: 2943-2973.
41. Ray P, De A, Min JJ, Tsien RY and Gambhir SS. Imaging tri-fusion multimodality reporter gene expression in living subjects. Cancer Res 2004; 64: 1323-1330.
42. Ray P, Tsien R and Gambhir SS. Construction and validation of improved triple fusion reporter gene vectors for molecular imaging of living subjects. Cancer Res 2007; 67: 3085-3093.
43. Ray P, Wu AM and Gambhir SS. Optical biolumi-nescence and positron emission tomography imaging of a novel fusion reporter gene in tu-mor xenografts of living mice. Cancer Res 2003; 63: 1160-1165.
44. Kesarwala AH, Prior JL, Sun J, Harpstrite SE, Sharma V and Piwnica-Worms D. Second-generation triple reporter for bioluminescence, micro-positron emission tomography, and fluo-rescence imaging. Mol Imaging 2006; 5: 465-474.
45. Luker GD, Luker KE, Sharma V, Pica CM, Dahl-heimer JL, Ocheskey JA, Fahrner TJ, Milbrandt J and Piwnica-Worms D. In vitro and in vivo char-acterization of a dual-function green fluores-cent protein--HSV1-thymidine kinase reporter gene driven by the human elongation factor 1 alpha promoter. Mol Imaging 2002; 1: 65-73.
46. Hong H, Yang Y and Cai W. Imaging gene ex-pression in live cells and tissues. Cold Spring Harb Protoc 2011; 2011: pdb top103.
47. Phelps ME. PET: the merging of biology and imaging into molecular imaging. J Nucl Med 2000; 41: 661-681.
48. Hong H, Yang Y, Zhang Y and Cai W. Non-invasive imaging of human embryonic stem cells. Curr Pharm Biotechnol 2010; 11: 685-692.
49. Hong H, Yang Y, Zhang Y and Cai W. Non-invasive cell tracking in cancer and cancer ther-apy. Curr Top Med Chem 2010; 10: 1237-1248.