Gene expression of muscarinic, tachykinin, and purinergic receptors in porcine bladder: Comparison with cultured cells

Frontiers in Pharmacology

Volumn 4, Issue NOV, 2013, Pages 1-7

  Bahadory, Forough ^a   Moore, Kate H ^a   Liu, Lu ^a   Burcher, Elizabeth ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

Cell culture; Myofibroblasts; Purinergic receptors; Receptors; Smooth muscle; Suburothelium; Urothelium

Indexed keywords

ACETYLCHOLINE; ALPHA SMOOTH MUSCLE ACTIN; BETA,GAMMA METHYLENEADENOSINE TRIPHOSPHATE; MESSENGER RNA; MUSCARINIC RECEPTOR; NEUROKININ A; PURINERGIC RECEPTOR; TACHYKININ RECEPTOR; VIMENTIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BLADDER; CELL CULTURE; CELL DIFFERENTIATION; CELLS BY BODY ANATOMY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DETRUSOR MUSCLE; FEMALE; FLUORESCENCE; GENE EXPRESSION; GENETIC MARKER; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; MYOFIBROBLAST; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SMOOTH MUSCLE FIBER; SUBUROTHELIUM; SWINE; UROTHELIAL CELL;

EID: 84920846938     PISSN: None     EISSN: 16639812     Source Type: Journal    
DOI: 10.3389/fphar.2013.00148     Document Type: Article

References (45)

1. Akino, H., Chapple, C. R., McKay, N., Cross, R. L., Murakami, S., Yokoyama, O., et al. (2008). Spontaneous contractions of the pig urinary bladder: the effect of ATP-sensitive potassium channels and the role of the mucosa. BJU Int. 102, 1168-1174. doi: 10.1111/j.1464-410X.2008.07782.x
2. Arrighi, N., Bodei, S., Zani, D., Michel, M. C., Simeone, C., Cosciani Cunico, S., et al. (2013). Different muscarinic receptor subtypes modulate proliferation of primary human detrusor smooth muscle cells via Akt/PI3K and map kinases. Pharmacol. Res. 74, 1-6. doi: 10.1016/j.phrs.2013.04.007
3. Birder, L. A. (2013). Nervous network for lower urinary tract function. Int. J. Urol. 20, 4-12. doi: 10.1111/j.1442-2042.2012.03210.x
4. Burnstock, G. (2007). Purine and pyrimidine receptors. Cell Mol. Life Sci. 64, 1471-1483. doi: 10.1007/s00018-007-6497-0
5. Burnstock, G. (2013). Purinergic signaling in the lower urinary tract. Acta Physiol. 207, 40-52. doi: 10.1111/apha.12012
6. Bushfield, M., Metcalfe, M., and Naylor, A. M. (1995). Activation of the micturition reflex by NK2 receptor stimulation in the anaesthetized guinea-pig. Br. J. Pharmacol. 115, 875-882. doi: 10.1111/j.1476-5381.1995.tb15891.x
7. Chancellor, M. B., Kaplan, S. A., and Blaivas, J. G. (1992). The cholinergic and purinergic components of detrusor contractility in a whole rabbit bladder model. J. Urol. 148, 906-909.
8. Cheng, Y., Mansfield, K., Sandow, S., Sadananda, P., Burcher, E., and Moore, K. H. (2011). Porcine bladder urothelial, myofibroblast, and detrusor muscle cells: characterization and ATP release. Front. Pharmacol. 2:27. doi: 10.3389/fphar.2011.00027
9. Chess-Williams, R., Chapple, C. R., Yamanishi, T., Yasuda, K., and Sellers, D. J. (2001). The minor population of M3-receptors mediate contraction of human detrusor muscle in vitro. J. Auton. Pharmacol. 21, 243-248. doi: 10.1046/j.1365-2680.2001.00231.x
10. Chiavegato, A., Roelofs, M., Franch, R., Castellucci, E., Sarinella, F., and Sartore, S. (1999). Differential expression of SM22 isoforms in myofibroblasts and smooth muscle cells from rabbit bladder. J. Muscle Res. Cell Motil. 20, 133-146. doi: 10.1023/A:1005411201187
11. Choppin, A., Eglen, R. M., and Hegde, S. S. (1998). Pharmacological characterization of muscarinic receptors in rabbit isolated iris sphincter muscle and urinary bladder smooth muscle. Br. J. Pharmacol. 124, 883-888. doi: 10.1038/sj.bjp.0701920
12. Dai, L., Perera, D. S., King, D. W., Southwell, B. R., Burcher, E., and Liu, L. (2012). Hemokinin-1 stimulates prostaglandin E2 production in human colon through activation of cyclooxygenase-2 and inhibition of 15- hydroxyprostaglandin dehydrogenase. J. Pharmacol. Exp. Ther. 340, 27-36. doi: 10.1124/jpet.111.186155
13. Darby, I., Skalli, O., and Gabbiani, G. (1990). Alpha-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing. Lab. Invest. 63, 21-29.
14. Darby, I. A., and Hewitson, T. D. (2007). Fibroblast differentiation in wound healing and fibrosis. Int. Rev. Cytol. 257, 143-179. doi: 10.1016/S0074-7696(07)57004-X
15. Fetscher, C., Fleichman, M., Schmidt, M., Krege, S., and Michel, M. C. (2002). M3 muscarinic receptors mediate contraction of human urinary bladder. Br. J. Pharmacol. 136, 641-643. doi: 10.1038/sj.bjp.0704781
16. Fry, C. H., Sui, G. P., Kanai, A. J., and Wu, C. (2007). The function of suburothelial myofibroblasts in the bladder. Neurourol. Urodyn. 26, 914-919. doi: 10.1002/nau.20483
17. Huber, A., and Badylak, S. F. (2012). Phenotypic changes in cultured smooth muscle cells: limitation or opportunity for tissue engineering of hollow organs. J. Tissue Eng. Regen. Med. 6, 505-511. doi: 10.1002/term.451
18. Ishizuka, O., Mattiasson, A., and Andersson, K. E. (1995). Tachykinin effects on bladder activity in conscious normal rats. J. Urol. 154, 257-261. doi: 10.1016/S0022-5347(01)67290-2
19. Kumar, V., Chapple, C. C., and Chess-Williams, R. (2004). Characteristics of adenosine triphosphate release from porcine and human normal bladder. J. Urol. 172, 744-747. doi: 10.1097/01.ju.0000131244.67160.f4ABSTRACT
20. Liu, L., Markus, I., Saghire, H. E., Perera, D. S., King, D. W., and Burcher, E. (2011). Distinct differences in tachykinin gene expression in ulcerative colitis, Crohn's disease, and diverticular disease: a role for hemokinin-1. Neurogastroent. Motil. 23, 475-483. doi: 10.1111/j.1365-2982.2011.01685.x
21. Longhurst, P. A., Schwegel, T., Folander, K., and Swanson, R. (1996). The human P2X1 receptor: molecular cloning, tissue distribution, and localization to chromosome 17. Biochim. Biophys. Acta 1308, 185-188. doi: 10.1016/0167-4781(96)00112-1
22. Maggi, C. A., Geppetti, P., Santicioli, P., Frilli, S., Giuliani, S., Furio, M., et al. (1988). Tachykinin-like immunoreactivity in the mammalian urinary bladder: correlation with the functions of the capsaicin-sensitive sensory nerves. Neuroscience. 26, 233-242. doi: 10.1016/0306-4522(88)90140-6
23. Maggi, C. A., Patacchini, R., Santicioli, P., and Giuliani, S. (1991). Tachykinin antagonists and capsaicin-induced contraction of the rat isolated urinary bladder: evidence for tachykinin-mediated cotransmission. Br. J. Pharmacol. 103, 1535-1541. doi: 10.1111/j.1476-5381.1991.tb09823.x
24. Mansfield, K. J., Liu, L., Mitchelson, F. J., Moore, K. H., Millard, R. J., and Burcher, E. (2005). Muscarinic receptor subtypes in human bladder detrusor and mucosa, studied by radioligand binding and quantitative competitive RT-PCR: changes in ageing. Br. J. Pharmacol. 144, 1089-1099. doi: 10.1038/sj.bjp.0706147
25. McCloskey, K. D. (2010). Interstitial cells in the urinary bladder-localization and function. Neurourol. Urodyn. 29, 82-87. doi: 10.1002/nau.20739
26. Mussap, C. J., Stamatakos, C., and Burcher, E. (1996). Radioligand binding, autoradiographic and functional studies demonstrate tachykinin NK-2 receptors in dog urinary bladder. J. Pharmacol. Exp. Ther. 279, 423-434.
27. Nair, D. G., Han, T. Y., Lourenssen, S., and Blennerhassett, M. G. (2011). Proliferation modulates intestinal smooth muscle phenotype in vitro and in colitis in vivo. Am. J. Physiol. Gastrointest Liver Physiol. 300, 903-913. doi: 10.1152/ajpgi.00528.2010
28. Ochodnicky, P., Uvelius, B., Andersson, K. E., and Michel, M. C. (2013). Autonomic nervous control of the urinary bladder. Acta Physiol. 207, 16-33. doi: 10.1111/apha.12010
29. Otomo, R., Shimoda, N., Satoh, S., Sato, K., Ogawa, O., and Kato, T. (1999). The role of ATP-receptor in controlling the urinary bladder and urethral function in rats. Nihon Hinyokika Gakkai Zasshi. 90, 681-687.
30. Pfaffl, M. W. (2001). A new mathematical model for relative quantification in realtime RT-PCR. Nucleic Acids Res. 29, 45-49. doi: 10.1093/nar/29.9.e45
31. Sadananda, P., Chess-Williams, R., and Burcher, E. (2008). Contractile properties of the pig bladder mucosa in response to neurokinin A: a role for myofibroblasts. Br. J. Pharmacol. 153, 1465-1473. doi: 10.1038/bjp.2008.29
32. Sadananda, P., Kao, F. C., Liu, L., Mansfield, K. J., and Burcher, E. (2012). Acid and stretch, but not capsaicin, are effective stimuli for ATP release in the porcine bladder mucosa: are ASIC and TRPV1 receptors involved. Eur. J. Pharmacol. 683, 252-259. doi: 10.1016/j.ejphar.2012.02.041
33. Sadananda, P., Shang, F., Liu, L., Mansfield, K. J., and Burcher, E. (2009). Release of ATP from rat urinary bladder mucosa: role of acid, vanilloids, and stretch. Br. J. Pharmacol. 158, 1655-1662. doi: 10.1111/j.1476-5381.2009.00431.x
34. Salvatori, G., Lattanzi, L., Coletta, M., Aguanno, S., Vivarelli, E., Kelly, R., et al. (1995). Myogenic conversion of mammalian fibroblasts induced by differentiating muscle cells. J. Cell Sci. 108, 2733-2739.
35. Sellers, D. J., Yamanishi, T., Chapple, C. R., Couldwell, C., Yasuda, K., and Chess-Williams, R. (2000). M3 muscarinic receptors but not M2 mediate contraction of the porcine detrusor muscle in vitro. J. Auton. Pharmacol. 20, 171-176. doi: 10.1046/j.1365-2680.2000.00181.x
36. Sui, G. P., Wu, C., and Fry, C. H. (2006). Characterization of the purinergic receptor subtype on guinea-pig suburothelial myofibroblasts. BJU Int. 97, 1327-1331. doi: 10.1111/j.1464-410X.2006.06200.x
37. Templeman, L., Sellers, D. J., Chapple, C. R., Rosario, D. J., Hay, D. P., and Chess-Williams, R. (2003). Investigation of neurokinin-2 and -3 receptors in the human and pig bladder. BJU Int. 92, 787-792. doi: 10.1046/j.1464-410X.2003.04458.x
38. Tong, Y. C., Hung, Y. C., and Cheng, J. T. (1997). Evidence of P2Y-purinoceptor mediated bladder neck smooth muscle post-contractile relaxation in the male mini-pig. Neurosci. Lett. 225, 181-184. doi: 10.1016/S0304-3940(97)00212-7
39. Vial, C., and Evans, R. J. (2000). P2X receptor expression in mouse urinary bladder and the requirement of P2X1 receptors for functional P2X receptor responses in the mouse urinary bladder smooth muscle. Br. J. Pharmacol. 131, 1489-1495. doi: 10.1038/sj.bjp.0703720
40. Whitbeck, C., Chichester, P., Sokol, R., and Levin, R. M. (2007). Role of nitric oxide in urinary bladder function: effect of L-arginine. Urol. Int. 78, 30-36. doi: 10.1159/000096931
41. Wiseman, O. J., Fowler, C. J., and Landon, D. N. (2003). The role of the human bladder lamina propria myofibroblast. BJU Int. 91, 89-93. doi: 10.1046/j.1464-410X.2003.03802.x
42. Wu, C., Sui, G. P., and Fry, C. H. (2004). Purinergic regulation of guinea pig suburothelial myofibroblasts. J. Physiol. 559, 231-243. doi: 10.1113/jphysiol.2004.067934
43. Yoshida, M., Masunaga, K., Nagata, T., Maeda, Y., Miyamoto, Y., Kudoh, J., et al. (2009). Attenuation of non-neuronal adenosine triphosphate release from human bladder mucosa by antimuscarinic agents. LUTS. 1, 88-92. doi: 10.1111/j.1757-5672.2009.00049.x
44. Yoshimura, N., Kaiho, Y., Miyazato, M., Yunoki, T., Tai, C., Chancellor, M. B., et al. (2008). Therapeutic receptor targets for lower urinary tract dysfunction. Naunyn Schmiedebergs Arch. Pharmacol. 377, 437-448. doi: 10.1007/s00210-007-0209-z
45. Yu, W., Sun, X., Robson, S. C., and Hill, W. G. (2013). Extracellular UDP enhances P2X-mediated bladder smooth muscle contractility via P2Y6 activation of the phospholipase C/inositol trisphosphate pathway. FASEB J. 27, 1895-1903. doi: 10.1096/fj.12-219006