Extreme PCR: Efficient and specific DNA amplification in 15-60 seconds

Clinical Chemistry

Volumn 61, Issue 1, 2015, Pages 145-153

  Farrar, Jared S ^a,b   Wittwer, Carl T ^a  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^b VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGAROSE; GENOMIC DNA; INTERLEUKIN 10 RECEPTOR BETA; POTASSIUM CHANNEL KCNE1; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) DEHYDROGENASE (QUINONE); REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) DEHYDROGENASE (QUINONE) 1; UNCLASSIFIED DRUG; DNA; IL10RB PROTEIN, HUMAN; KCNE1 PROTEIN, HUMAN; NQO1 PROTEIN, HUMAN; VOLTAGE GATED POTASSIUM CHANNEL;

AMPLICON; ARTICLE; CONTROLLED STUDY; EXTREME POLYMERASE CHAIN REACTION; GEL ELECTROPHORESIS; GENOTYPE; HIGH RESOLUTION MELTING ANALYSIS; HUMAN; HYPODERMIC NEEDLE; MOLECULAR WEIGHT; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; TEMPERATURE; DEVICES; DNA TEMPLATE; GENETICS; HEAT; HUMAN GENOME; MOLECULAR DIAGNOSIS; NUCLEIC ACID AMPLIFICATION; POLYMERASE CHAIN REACTION; PROCEDURES; TIME;

DNA; GENOME, HUMAN; HOT TEMPERATURE; HUMANS; INTERLEUKIN-10 RECEPTOR BETA SUBUNIT; MOLECULAR DIAGNOSTIC TECHNIQUES; NAD(P)H DEHYDROGENASE (QUINONE); NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; POTASSIUM CHANNELS, VOLTAGE-GATED; TEMPLATES, GENETIC; TIME FACTORS;

EID: 84920507071     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2014.228304     Document Type: Article

References (26)

1. Mullis K, Ferre F, Gibbs R, eds. The polymerase chain reaction. Boston: Birkhauser; 1994.
2. Bustin SA, ed. The PCR revolution: basic technologies and applications. New York: Cambridge University Press; 2010.
3. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988;239:487-91.
4. Wittwer CT, Fillmore GC, Garling DJ. Minimizing the time required for DNA amplification by efficient heat transfer to small samples. Anal Biochem 1990;186: 328-31.
5. Wittwer CT, Garling DJ. Rapid cycle DNA amplification: time and temperature optimization. Biotechniques 1991;10:76-83.
6. Zhang C, Xing D. Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends. Nucl Acid Res 2007;35:4223-37.
7. Roper MG, Easley CJ, Landers JP. Advances in polymerase chain reaction on microfluidic chips. Anal Chem 2005;77:3887-93.
8. Wheeler EK, Hara CA, Frank J, Deotte J, Hall SB, Benett W, et al. Under-three minute PCR: probing the limits of fast amplification. Analyst 2011;136:3707-12.
9. Terazono H, Takei H, Hattori A, Yasuda K. Development of a high-speed real-time polymerase chain reaction system using a circulating water-based rapid heatexchange. Jpn J Appl Phys 2010;49:06GM05.
10. Huhmer AFR, Landers JP. Noncontact infraredmediated thermocycling for effective polymerase chain reaction amplification of DNA in nanoliter volumes. Anal Chem 2000;72:5507-12.
11. Fuchiwaki Y, Nagai H, Saito M, Tamiya E. Ultra-rapid flow-through polymerase chain reaction microfluidics using vapor pressure. Biosens Bioelectron 2011;27: 88-94.
12. Maltezos G, Johnston M, Taganov K, Srichantaratsamee C, Gorman J, Baltimore D, et al. Exploring the limits of ultrafast polymerase chain reaction using liquid for thermal heat exchange: a proof of principle. Appl Phys Lett 2010;97:264101.
13. Kopp MU, Mello AJ, Manz A. Chemical amplification: continuous-flow PCR on a chip. Science 1998;280: 1046-8.
14. Hashimoto M, Chen PC, Mitchell MW, Nikitopoulos DE, Soper SA, Murphy MC. Rapid PCR in a continuous flow device. Lab Chip 2004;4:638-45.
15. Palais R, Wittwer CT. Mathematical algorithms for highresolution DNA melting analysis. Meth Enzymol 2009; 454:323-43.
16. Higuchi R, Fockler C, Dollinger G, Watson R. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnol 1993;11:1026-30.
17. Pancholi R, Kelly C, Raczkowski M, Balada-Llasat JM. Detection of toxigenic Clostridium difficile: comparison of the cell culture neutralization, Xpert C. Difficile, Xpert C. Difficile/Epi, and Illumigene C. Difficile assays. J Clin Microbiol 2012;50:1331-5.
18. Poritz MA, Blaschke AJ, Byington CL, Meyers L, Nilsson K, Jones DE, et al. FilmArray, an automated nested multiplex PCR system for multi-pathogen detection: development and application to respiratory tract infection. PLoS ONE 2011;6:e26047.
19. Wetmur JG, Davidson N. Kinetics of renaturation of DNA. J Mol Biol 1968;31:349-70.
20. Wittwer CT, Herrmann MG. Rapid thermal cycling and PCR kinetics. In: Innis M, Gelfand D, Sninsky J, eds. PCR methods manual. San Diego: Academic Press; 1999: 211-29.
21. Wittwer CT, Marshall BC, Reed GH, Cherry JL. Rapid cycle allele-specific amplification: studies with the cystic fibrosis delta F508 locus. Clin Chem 1993;39:804-9.
22. Montgomery JL, Rejali N, Wittwer CT. Stopped-flow DNA polymerase assay by continuous monitoring of dNTP incorporation by fluorescence. Anal Biochem 2013;441:133-9.
23. Montgomery JL, Wittwer CT. Influence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instruments. Clin Chem 2014;60:334-40.
24. Wittwer CT, Kusukawa N. Real-time PCR and melting analysis. In: Persing DH, Tenover FC, Hayden R, Nolte F, Tang YW, Belkum AV, eds. Molecular microbiology: diagnostic principles and practice. Washington (DC): ASM Press; 2011. p 63-82.
25. Sanford LN, Wittwer CT. Monitoring temperature with fluorescence during real-time PCR and melting analysis. Anal Biochem 2013;434:26-33.
26. Vossen RH, Aten E, Roos A, den Dunnen JT. Highresolution melting analysis (HRMA): more than just sequence variant screening. Hum Mutat 2009;30: 860-6.