Monitoring temperature with fluorescence during real-time PCR and melting analysis

Analytical Biochemistry

Volumn 434, Issue 1, 2013, Pages 26-33

  Sanford, Lindsay N ^a   Wittwer, Carl T ^b,c  

^a Department of Electrical and Computer Engineering   (United States)

^b UNIVERSITY OF UTAH HEALTH SCIENCES CENTER   (United States)

^c ARUP INSTITUTE FOR CLINICAL AND EXPERIMENTAL PATHOLOGY   (United States)

Author keywords

Fluorescence; Polymerase chain reaction; Real time PCR; Temperature control; Thermal cycling

Indexed keywords

FLUORESCENCE; MELTING; PHOTOBLEACHING; TEMPERATURE CONTROL; THERMAL CYCLING; THERMOCOUPLES;

CALIBRATION CURVES; INTRINSIC FLUORESCENCE; RAPID TEMPERATURE; REAL-TIME PCR; SAMPLE TEMPERATURE; SOLUTION TEMPERATURE; TARGET TEMPERATURE; TEMPERATURE SENSITIVE;

POLYMERASE CHAIN REACTION;

SULFORHODAMINE B;

ACCURACY; ARTICLE; ARTIFACT; CONTROLLED STUDY; EVAPORATION; FLUORESCENCE; HYSTERESIS; MELTING POINT; PRIORITY JOURNAL; PROTEIN DENATURATION; REAL TIME POLYMERASE CHAIN REACTION; TEMPERATURE MEASUREMENT; TRANSITION TEMPERATURE;

EID: 84872847183     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.10.037     Document Type: Article

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