Stopped-flow DNA polymerase assay by continuous monitoring of dNTP incorporation by fluorescence

Analytical Biochemistry

Volumn 441, Issue 2, 2013, Pages 133-139

  Montgomery, Jesse L ^a   Rejali, Nick ^a   Wittwer, Carl T ^a  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

Activity assay; Intercalating dye; Polymerase; Stopped flow

Indexed keywords

CHLORINE COMPOUNDS; DNA; MAGNESIUM COMPOUNDS; POLYMERS; POTASSIUM COMPOUNDS; SUBSTRATES;

ACCURATE MEASUREMENT; ACTIVITY ASSAYS; CONTINUOUS MONITORING; INTERCALATING DYE; POLYMERASE; POLYMERASE ACTIVITIES; RADIOLABELED SUBSTRATES; STOPPED FLOW;

POLYMERASE CHAIN REACTION;

DNA POLYMERASE; DOUBLE STRANDED DNA; TAQ POLYMERASE;

AMINO ACID SEQUENCE; ARTICLE; ASSAY; CALIBRATION; CONCENTRATION (PARAMETERS); FLUORESCENCE IMAGING; NONHUMAN; PH; PRIORITY JOURNAL; STOPPED FLOW ASSAY; THERMOCOCCUS KODAKARENSIS;

ACTIVITY ASSAY; INTERCALATING DYE; POLYMERASE; STOPPED-FLOW;

DNA; DNA-DIRECTED DNA POLYMERASE; ENZYME ASSAYS; FLUORESCENCE; NUCLEOTIDES;

EID: 84884940188     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.07.008     Document Type: Article

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