Simple amino acid tags improve both expression and secretion of Candida antarctica lipase B in recombinant Escherichia coli

Biotechnology and Bioengineering

Volumn 112, Issue 2, 2015, Pages 346-355

  Kim, Sun Ki ^a   Park, Yong Cheol ^b   Lee, Hyung Ho ^b,c   Jeon, Seung Taeg ^b,c   Min, Won Ki ^a   Seo, Jin Ho ^a  

^a Seoul National University   (South Korea)

^b Kookmin University   (South Korea)

^c Seoul National University   (South Korea)

Author keywords

Candida antarctica lipase b; Escherichia coli; Extracellular secretion; Polyanionic amino acid tag; Quaternary structure change

Indexed keywords

CANDIDA; ENZYME ACTIVITY; ESCHERICHIA COLI; ISOMERS; PHYSIOLOGY; YEAST;

BIOCHEMICAL REACTIONS; CANDIDA ANTARCTICA LIPASE B; ENZYMATIC PROPERTIES; EXTRACELLULAR SECRETION; HETEROLOGOUS PROTEINS; POLYANIONICS; QUATERNARY STRUCTURE; RECOMBINANT ESCHERICHIA COLI;

AMINO ACIDS;

AMINO ACID; FUNGAL PROTEIN; HYBRID PROTEIN; LIPASE B, CANDIDA ANTARCTICA; TRIACYLGLYCEROL LIPASE;

CHEMICAL STRUCTURE; CHEMISTRY; ESCHERICHIA COLI; GENETICS; METABOLISM; PROTEIN QUATERNARY STRUCTURE; SECRETION (PROCESS);

AMINO ACIDS; ESCHERICHIA COLI; FUNGAL PROTEINS; LIPASE; MODELS, MOLECULAR; PROTEIN STRUCTURE, QUATERNARY; RECOMBINANT FUSION PROTEINS;

EID: 84920062924     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25361     Document Type: Article

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