Polycationic amino acid tags enhance soluble expression of Candida antarctica lipase B in recombinant Escherichia coli

Bioprocess and Biosystems Engineering

Volumn 34, Issue 7, 2011, Pages 833-839

  Jung, Hyun Jung ^a   Kim, Sun Ki ^a   Min, Won Ki ^a   Lee, Sung Suk ^b   Park, Kyungmoon ^c   Park, Yong Cheol ^d   Seo, Jin Ho ^a  

^a Seoul National University   (South Korea)

^b Korea Forest Research Institute   (South Korea)

^c Hong Ik University   (South Korea)

^d Kookmin University   (South Korea)

Author keywords

Candida antarctica lipase B; Escherichia coli; Inclusion body; Polycationic amino acid tag; Soluble expression

Indexed keywords

BIOCHEMICAL REACTIONS; CANDIDA ANTARCTICA LIPASE B; E. COLI; INCLUSION BODIES; INCLUSION BODY; LOW LEVEL; POLYCATIONIC AMINO ACID TAG; RECOMBINANT E. COLI; RECOMBINANT ESCHERICHIA COLI; SOLUBLE EXPRESSION; SPECIFIC ACTIVITY; TOTAL PROTEIN;

ARGININE; CANDIDA; ISOMERS; SOAPS (DETERGENTS); YEAST;

ESCHERICHIA COLI;

AMINO ACID; ARGININE; LIPASE B; LYSINE; POLYCATIONIC AMINO ACID TAG; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL STRAIN; CANDIDA ANTARCTICA; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENETIC MANIPULATION; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SOLUBILITY;

AMINO ACIDS; ESCHERICHIA COLI; INCLUSION BODIES; LIPASE; PEPTIDES; POLYAMINES; POLYLYSINE; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

CANDIDA ANTARCTICA; ESCHERICHIA COLI;

EID: 80052378833     PISSN: 16157591     EISSN: 16157605     Source Type: Journal    
DOI: 10.1007/s00449-011-0533-z     Document Type: Article

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