The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins

Journal of Bacteriology

Volumn 178, Issue 12, 1996, Pages 3544-3549

  Francetic, Olivera ^a,b   Pugsley, Anthony P ^a  

^a CNRS   (France)

^b UNIVERSITY OF BELGRADE   (Serbia)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA; ESCHERICHIA COLI K12; KLEBSIELLA OXYTOCA; NEISSERIA GONORRHOEAE; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

PULLULANASE;

ARTICLE; BINDING SITE; CHROMOSOME; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; OPERON; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN SYNTHESIS; SEQUENCE ANALYSIS;

BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; BASE SEQUENCE; DNA, BACTERIAL; ENDOPEPTIDASES; ESCHERICHIA COLI; FIMBRIAE PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENETIC COMPLEMENTATION TEST; GLYCOSIDE HYDROLASES; MOLECULAR SEQUENCE DATA; OPERON; PROMOTER REGIONS (GENETICS); REGULATORY SEQUENCES, NUCLEIC ACID; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

EID: 0029924909     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.12.3544-3549.1996     Document Type: Article

References (30)

1. Andrews, S. C., P. M. Harrison, and J. R. Guest. 1989. Cloning, sequencing, and mapping of the bacterioferritin gene (bfr) of Escherichia coli K-12. J. Bacteriol. 171:3940-3947.
2. Condemine, G., C. Dorel, N. Hugouvieux-Cotte-Pattat, and J. Robert-Baudouy. 1992. Some of the out genes involved in the secretion of pectate lyases in Erwinia chrysanthemi are regulated by kdgR. Mol. Microhiol. 6:3199-3211.
3. d'Enfert, C., and A. P. Pugsley. 1989. Klebsiella pneumoniae pulS gene encodes an outer membrane lipoprotein required for pullulanase secretion. J. Bacteriol. 171:3673-3679.
4. d'Enfert, C., I. Reyss, C. Wanderman, and A. P. Pugsley. 1989. Protein secretion by Gram-negative bacteria: characterization of two membrane proteins required for pullulanase secretion by Escherichia coli K-12. J. Biol. Chem. 264:17462-17468.
5. d'Enfert, C., A. Ryter, and A. P. Pugsley. 1987. Cloning and expression in Escherichia coli of the Klebsiella pneumoniae genes for production, surface localization and secretion of the lipoprotein pullulanase. EMBO J. 6:3531-3538.
6. Dupuy, B. 1994. Unpublished data.
7. Dupuy, B., and A. P. Pugsley. 1994. Type IV prepilin peptidase gene of Neisseria gonorrhoeae MS11: presence of a related gene in other piliated and nonpiliated Neisseria strains. J. Bacteriol. 176:1323-1331.
8. Hardie, K. R., S. Lory, and A. P. Pugsley. 1996. Insertion of an outer membrane protein in Escherichia coli requires a chaperone-like protein. EMBO J. 15:978-988.
9. Howard, S. P., J. Critch, and A. Bedi. 1993. Isolation and analysis of eight exe genes and their involvement in extracellular protein secretion and outer membrane assembly in Aeromonas hydrophila. J. Bacteriol. 175:6695-6703.
10. Jahagirdar, R., and S. P. Howard. 1994. Isolation and characterization of a second exe operon required for extracellular protein secretion in Aeromonas hydrophila. J. Bacteriol. 176:6819-6826.
11. Jiang, B., and S. P. Howard. 1992. The Aeromonas hydrophila exeE gene, required both for protein secretion and normal outer membrane biogenesis, is a member of a general secretion pathway. Mol. Microbiol. 6:1351-1361.
12. Lauer, P., N. H. Albertson, and M. Koomey. 1993. Conservation of genes encoding components of a type IV pilus assembly/two-step protein export pathway in Neisseria gonorrhoeae. Mol. Microbiol. 8:357-368.
13. Michaelis, S., C. Chapon, C. d'Enfert, A. P. Pugsley, and M. Schwartz. 1985. Characterization and expression of the structural gene for pullulanase, a maltose-inducible secreted protein of Klebsiella pneumoniae. J. Bacteriol. 164:633-638.
14. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
15. Nunn, D., and S. Lory. 1991. Product of the Pseudomonas aeruginosa gene pilD is a prepilin leader peptidase. Proc. Natl. Acad. Sci. USA 88:3281-3285.
16. Nunn, D., and S. Lory. 1992. Components of the protein excretion apparatus of Pseudomonas aeruginosa are processed by the type IV prepilin peptidase. Proc. Natl. Acad. Sci. USA 89:47-51.
17. Plunkett, G. 1995. Escherichia coli K-12 chromosomal region from 67.4 to 76.0 minutes. GenBank U18997. Locus ECOUW67.
18. Plunkett, G. 1995. Escherichia coli K-12 genome; approximately 65 to 68 minutes. GenBank U28377. Locus ECU28377.
19. Possot, O. 1994. Unpublished data.
20. Pugsley, A. P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol Rev. 57:50-108.
21. Pugsley, A. P. 1993. Processing and methylation of PulG. a pilin-like component of the general secretory pathway of Klebsiella oxytoca. Mol. Microbiol. 9:295-308.
22. Pugsley, A. P. Multimers of the precursor of a type IV pilin-like component of the general secretory pathway are unrelated to pili. Mol. Microbiol., in press.
23. Pugsley, A. P., and B. Dupuy. 1992. An enzyme with type IV prepilin peptidase activity is required to process a component of the general extracellular protein secretion pathway of Klebsiella oxyloca. Mol. Microbiol. 6:751-760.
24. Pugsley, A. P., and O. Possot. 1993. The general secretory pathway of Klebsiella oxyloca: no evidence for relocalization or assembly of pilin-like PulG protein into a multiprotein complex. Mol. Microbiol. 10:665-674.
25. Pugsley, A. P., and I. Reyss. 1990. Five genes at the 3′ end of the Klebsiella pneumoniae pulC operon are required for pullulanase secretion. Mol. Microbiol. 4:365-379.
26. Richet, E., and O. Raibaud. 1991. Supercoiling is essential for the formation and stability of the initiation complex at the divergent malEp and malKp promoters. J. Mol. Biol. 218:529-542.
27. Simons, R. W., F. Houman, and N. Kleckner. 1987. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53:85-96.
28. Stojiljkovic, I., R. Schönherr, and J. G. Kusters. 1995. Identification of the hopG gene, a component of Escherichia coli K-12 type II export system, and its conservation among different pathogenic Escherichia coli and Shigella isolates. J. Bacteriol. 177:1892-1895.
29. Takeshita, S., M. Sato, M. Toba, W. Masahashi, and T. Hashmoto-Goto. 1987. High copy number and low copy number plasmid vectors for lacZ α-complementation and chloramphenicol- or kanamycin-resistance selection. Gene 61:63-74.
30. Whitchurch, C. B., and J. S. Mattick. 1994. Escherichia coli contains a set of genes homologous to those involved in protein secretion, DNA uptake and the assembly of type-4 fimbriae in other bacteria. Gene 150:9-15.