Structure-based design and functional studies of novel noroviral 3C protease chimaeras offer insights into substrate specificity

Biochemical Journal

Volumn 464, Issue 3, 2014, Pages 461-472

  Herod, Morgan R ^a   Prince, Cynthia A ^a   Skilton, Rachel J ^a   Ward, Vernon K ^b   Cooper, Jonathan B ^c   Clarke, Ian N ^a  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^b UNIVERSITY OF OTAGO   (New Zealand)

^c UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Murine norovirus (MNV); Norovirus; Polyprotein; Positive sense; Protease; Proteolysis

Indexed keywords

HUMAN NOROVIRUS NON STRUCTURAL PROTEIN 6; POLYPROTEIN; PROTEINASE; UNCLASSIFIED DRUG; VIRUS PROTEIN; 3C PROTEASES; CYSTEINE PROTEINASE; HYBRID PROTEIN;

ARTICLE; CHIMERA; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME KINETICS; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; NOROVIRUS; OPEN READING FRAME; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; VIRUS GENOME; AMINO ACID SEQUENCE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; HEK293 CELL LINE; METABOLISM; X RAY CRYSTALLOGRAPHY;

CHIMAERIFORMES; ESCHERICHIA COLI; MURINE NOROVIRUS; NOROVIRUS;

AMINO ACID SEQUENCE; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; CYSTEINE ENDOPEPTIDASES; HEK293 CELLS; HUMANS; MODELS, MOLECULAR; MOLECULAR STRUCTURE; NOROVIRUS; PROTEOLYSIS; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY; VIRAL PROTEINS;

EID: 84919346833     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20140959     Document Type: Article

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