Fast and efficient drosophila melanogaster gene knock-ins using MiMIC transposons

G3: Genes, Genomes, Genetics

Volumn 4, Issue 12, 2014, Pages 2381-2387

  Vilain, Sven ^a,b   Vanhauwaert, Roeland ^a,b   Maes, Ine ^a,b   Schoovaerts, Nils ^a,b   Soukup, Lujia Zhou Sandra ^a,b   da Cunha, Raquel ^a,b   Lauwers, Elsa ^a,b   Fiers, Mark ^a,b   Verstreken, Patrik ^a,b  

^a UNIVERSITY OF LEUVEN   (Belgium)

^b DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

Author keywords

Drosophila; Genome editing; Homologous; MiMIc; Recombination

Indexed keywords

GENOMIC DNA; RESTRICTION ENDONUCLEASE; DROSOPHILA PROTEIN; LRRK PROTEIN, DROSOPHILA; PROTEIN SERINE THREONINE KINASE; TAU PROTEIN; TRANSPOSON;

ADULT; ALLELE; ARTICLE; CONTROLLED STUDY; DNA INTEGRATION; DNA MODIFICATION; DNA SEQUENCE; DOUBLE STRANDED DNA BREAK; DROSOPHILA MELANOGASTER; ENDOCYTOSIS; GENE CASSETTE; GENE LOCUS; GENE SEQUENCE; GENE TARGETING; GENOME; HOMOLOGOUS RECOMBINATION; NONHUMAN; OPEN READING FRAME; POLYMERASE CHAIN REACTION; SYNAPSE VESICLE; TRANSPOSON; WESTERN BLOTTING; ANIMAL; GENETICS;

DROSOPHILA MELANOGASTER;

ALLELES; ANIMALS; DNA TRANSPOSABLE ELEMENTS; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; GENE KNOCK-IN TECHNIQUES; HOMOLOGOUS RECOMBINATION; PROTEIN-SERINE-THREONINE KINASES; TAU PROTEINS;

EID: 84916935483     PISSN: None     EISSN: 21601836     Source Type: Journal    
DOI: 10.1534/g3.114.014803     Document Type: Article

References (25)

1. Bateman, J. R., A. M. Lee, and C. T. Wu, 2006 Site-specific transformation of Drosophila via phiC31 integrase-mediated cassette exchange. Genetics 173: 769-777.
2. Bibikova, M., M. Golic, K. G. Golic, and D. Carroll, 2002 Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases. Genetics 161: 1169-1175.
3. Bibikova, M., K. Beumer, J. K. Trautman, and D. Carroll, 2003 Enhancing gene targeting with designed zinc finger nucleases. Science 300: 764.
4. Bier, E., 2005 Drosophila, the golden bug, emerges as a tool for human genetics. Nat. Rev. Genet. 6: 9-23.
5. Bischof, J., R. K. Maeda, M. Hediger, F. Karch, and K. Basler, 2007 An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases. Proc. Natl. Acad. Sci. USA 104: 3312-3317.
6. Chan, C. C., S. Scoggin, P. R. Hiesinger, and M. Buszczak, 2012 Combining recombineering and ends-out homologous recombination to systematically characterize Drosophila gene families: Rab GTPases as a case study. Commun. Integr. Biol. 5: 179-183.
7. Chan, Y. S., D. S. Huen, R. Glauert, E. Whiteway, and S. Russell, 2013 Optimising homing endonuclease gene drive performance in a semi-refractory species: the Drosophila melanogaster experience. PLoS ONE 8: e54130.
8. Choi, C. M., S. Vilain, M. Langen, S. Van Kelst, N. De Geest et al. 2009 Conditional mutagenesis in Drosophila. Science 324: 54.
9. Cong, L., F. A. Ran, D. Cox, S. Lin, R. Barretto et al. 2013 Multiplex genome engineering using CRISPR/Cas systems. Science 339: 819-823.
10. FlyBase Genome, 2013 Changes affecting gene model number or type in release 5.51 of the annotated D. melanogaster genome. http://flybase.org/ reports/FBrf0220804.html.
11. Gao, G., C. McMahon, J. Chen, and Y. S. Rong, 2008 A powerful method combining homologous recombination and site-specific recombination for targeted mutagenesis in Drosophila. Proc. Natl. Acad. Sci. USA 105: 13999-14004.
12. Gratz, S. J., A. M. Cummings, J. N. Nguyen, D. C. Hamm, L. K. Donohue et al. 2013 Genome engineering of Drosophila with the CRISPR RNAguided Cas9 nuclease. Genetics 194: 1029-1035.
13. Gratz, S. J., F. P. Ukken, C. D. Rubinstein, G. Thiede, L. K. Donohue et al. 2014 Highly specific and efficient CRISPR/Cas9-catalyzed homologydirected repair in Drosophila. Genetics 196: 961-971.
14. Hsu, P. D., D. A. Scott, J. A. Weinstein, F. A. Ran, S. Konermann et al. 2013 DNA targeting specificity of RNA-guided Cas9 nucleases. Nat. Biotechnol. 31: 827-832.
15. Huang, J., W. Zhou, W. Dong, A. M. Watson, and Y. Hong, 2009 From the cover: directed, efficient, and versatile modifications of the Drosophila genome by genomic engineering. Proc. Natl. Acad. Sci. USA 106: 8284- 8289.
16. Jinek, M., K. Chylinski, I. Fonfara, M. Hauer, J. A. Doudna et al. 2012 A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337: 816-821.
17. Liu, J., C. Li, Z. Yu, P. Huang, H. Wu et al. 2012 Efficient and specific modifications of the Drosophila genome by means of an easy TALEN strategy. J. Genet. Genomics 39: 209-215.
18. Mali, P., J. Aach, P. B. Stranges, K. M. Esvelt, M. Moosburner et al. 2013 CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat. Biotechnol. 31: 833-838.
19. Pattanayak, V., S. Lin, J. P. Guilinger, E. Ma, J. A. Doudna et al. 2013 Highthroughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat. Biotechnol. 31: 839-843.
20. Rong, Y. S., and K. G. Golic, 2000 Gene targeting by homologous recombination in Drosophila. Science 288: 2013-2018.
21. Rong, Y. S., S. W. Titen, H. B. Xie, M. M. Golic, M. Bastiani et al. 2002 Targeted mutagenesis by homologous recombination in D. melanogaster. Genes Dev. 16: 1568-1581.
22. Venken, K. J., K. L. Schulze, N. A. Haelterman, H. Pan, Y. He et al. 2011 MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nat. Methods 8: 737-743.
23. Weng, R., Y. W. Chen, N. Bushati, A. Cliffe, and S. M. Cohen, 2009 Recombinase-mediated cassette exchange provides a versatile platform for gene targeting: knockout of miR-31b. Genetics 183: 399-402.
24. Wesolowska, N., and Y. S. Rong, 2013 Long-range targeted manipulation of the Drosophila genome by site-specific integration and recombinational resolution. Genetics 193: 411-419.
25. Yamamoto, S., M. Jaiswal, W. L. Charng, T. Gambin, E. Karaca et al. 2014 A Drosophila genetic resource of mutants to study mechanisms underlying human genetic diseases. Cell 159: 200-214.