Targeted mutagenesis by homologous recombination in D. melanogaster

Genes and Development

Volumn 16, Issue 12, 2002, Pages 1568-1581

  Rong, Yikang S ^a,b,d   Titen, Simon W ^a,b   Xie, Heng B ^a,b   Golic, Mary M ^a,b   Bastiani, Michael ^a   Bandyopadhyay, Pradip ^a   Olivera, Baldomero M ^a   Brodsky, Michael ^c,e   Rubin, Gerald M ^c   Golic, Kent G ^a,b  

^a UNIVERSITY OF UTAH   (United States)

^b STOWERS INSTITUTE FOR MEDICAL RESEARCH   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d NATIONAL CANCER INSTITUTE   (United States)

^e UNIVERSITY OF MASSACHUSETTS   (United States)

Author keywords

Drosophila; FLP; Gene targeting; I SceI; Recombination

Indexed keywords

DNA; ENDONUCLEASE; PROTEIN P53; RECOMBINASE;

ALLELE; ARTICLE; DROSOPHILA MELANOGASTER; FEMALE; GENE INSERTION; GENE LOCUS; GENE TARGETING; HOMOLOGOUS RECOMBINATION; MALE; MUTAGENESIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; TELOMERE; TRANSGENE; TUMOR SUPPRESSOR GENE;

ALLELES; ANIMALS; BLOTTING, SOUTHERN; DNA; DNA NUCLEOTIDYLTRANSFERASES; DROSOPHILA MELANOGASTER; FEMALE; GENES, P53; GENOME; HOMOZYGOTE; MALE; MODELS, GENETIC; MUTAGENESIS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHENOTYPE; PLASMIDS; POINT MUTATION; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); RECOMBINATION, GENETIC;

DROSOPHILA MELANOGASTER; INSERTION SEQUENCES; MELANOGASTER;

EID: 0037097866     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.986602     Document Type: Article

References (45)

1. The genome sequence of Drosophila melanogaster
2. In search of a function
3. I-PpoI and I-CreI homing site sequence degeneracy determined by random mutagenesis and sequential in vitro enrichment
4. Targeted recovery of mutations in Drosophila
5. Homologous recombination in mammalian cells
6. Drosophila p53 binds a damage response element at the reaper locus
7. Homologous genetic recombination in Xenopus: Mechanism and implications for gene manipulation
8. Green fluorescent protein as a marker for gene expression
9. DNA end-joining: From yeast to man
10. Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus
11. Reversal of fortune for Drosophila geneticists?
12. Vermillion as a small selectable marker gene for Drosophila transformation
13. The molecular biology of the SIR proteins
14. Gene-targeting in Drosophila validated
15. Targeted gene replacement in Drosophila via P element-induced gap repair
16. The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome
17. A quantitative measure of the mitotic pairing of alleles in Drosophila melanogaster and the influence of structural heterozygosity
18. Effect of chromosomal locus GC content and length of homology on PCR-mediated targeted gene replacement in Saccharomyces
19. Identification of a Drosophila vitamin K-dependent γ-glutamyl carboxylase
20. Intermolecular recombination between DNAs introduced into mouse L cells is mediated by a nonconservative pathway that leads to crossover products
21. Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: A general strategy for targeting mutations to non-selectable genes
22. Involvement of single-stranded tails in homologous recombination of DNA injected into Xenopus laevis oocyte nuclei
23. Ten years of gene targeting: Targeted mouse mutants, from vector design to phenotype analysis
24. Drosophila p53 is a structural and functional homolog of the tumor suppressor p53
25. Parameters controlling the rate of gene targeting frequency in the protozoan parasite Leishmania
26. Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae
27. Regulation of HSP70 synthesis by messenger RNA degradation
28. Ku DNA end-binding protein modulates homologous repair of double-strand breaks in mammalian cells
29. Dominant defects in Drosophila eye pigmentation resulting from a euchromatic-heterochromatic fusion gene
30. Gene targeting by homologous recombination in Drosophila
31. A targeted gene knockout in Drosophila
32. Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeast
33. Genetic transformation of Drosophila with transposable element vectors
34. Efficient repair of HO-induced chromosomal breaks in Saccharomyces cerevisiae by recombination between flanking homologous sequences
35. Characterization of two novel lipocalins expressed in the Drosophila embryonic nervous system
36. Shuttle mutagenesis: A method of transposon mutagenesis for Saccharomyces cerevisiae
37. Genetic analysis of the Chlamydomonas reinhardtii I-CreI mobile intron homing system in Escherichia coli
38. The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes
39. The double-strand-break repair model for recombination
40. Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs
41. Cleavage and recognition pattern of a double-strand-specific endonuclease (I-CreI) encoded by the chloroplast 23S rRNA intron of Chlamydomonas reinhardtii
42. On a potential global role for vitamin K-dependent γ-carboxylation in animal systems: Evidence for a γ-glutamyl carboxylase in Drosophila
43. Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis
44. Differential effects of Rad52p overexpression on gene targeting and extrachromosomal homologous recombination in a human cell line