Properties and clinical relevance of MTA1 protein in human cancer

Cancer and Metastasis Reviews

Volumn 33, Issue 4, 2014, Pages 891-900

  Toh, Yasushi ^a   Nicolson, Garth L ^b  

^a NATIONAL KYUSHU CANCER CENTER   (Japan)

^b INSTITUTE FOR MOLECULAR MEDICINE   (United States)

Author keywords

Clinical relevance; Human cancers; Metastasis associated gene protein family; NuRD complex; Overexpression

Indexed keywords

CARRIER PROTEINS AND BINDING PROTEINS; COMPLEMENTARY DNA; HISTONE DEACETYLASE; METASTASIS ASSOCIATED PROTEIN 1; NURD COMPLEX; PROTEIN; TUMOR PROTEIN; UNCLASSIFIED DRUG; MTA1 PROTEIN, HUMAN; PROTEOME; REPRESSOR PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CANCER CELL LINE; CANCER DIAGNOSIS; CANCER THERAPY; CELLULAR DISTRIBUTION; DEACETYLATION; GENE EXPRESSION; HUMAN; NEOPLASM; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN TARGETING; BIOSYNTHESIS; CHROMATIN ASSEMBLY AND DISASSEMBLY; GENE EXPRESSION REGULATION; GENETICS; PATHOPHYSIOLOGY; SIGNAL TRANSDUCTION;

CHROMATIN ASSEMBLY AND DISASSEMBLY; GENE EXPRESSION REGULATION, NEOPLASTIC; HISTONE DEACETYLASES; HUMANS; MI-2 NUCLEOSOME REMODELING AND DEACETYLASE COMPLEX; NEOPLASMS; PROTEOME; REPRESSOR PROTEINS; SIGNAL TRANSDUCTION;

EID: 84914173014     PISSN: 01677659     EISSN: 15737233     Source Type: Journal    
DOI: 10.1007/s10555-014-9516-2     Document Type: Article

References (85)

1. Kumar, R., Wang, R. A., & Bagheri-Yarmand, R. (2003). Emerging roles of MTA family members in human cancers. Seminars in Oncology, 30(Suppl 16), 30–37.
2. Manavathi, B., Singh, K., & Kumar, R. (2007). MTA family of coregulators in nuclear receptor biology and pathology. Nuclear Receptor Signaling, 5, 1–8.
3. Toh, Y., & Nicolson, G. L. (2009). The role of the MTA family and their encoded proteins in human cancers: molecular functions and clinical implications. Clinical and Experimental Metastasis, 26(3), 215–227.
4. Toh, Y., & Nicolson, G. L. (2011). MTA1 of the MTA (metastasis-associated) gene family and its encoded proteins: molecular and regulatory functions and its role in human cancer progression. Atlas of Genetics and Cytogenetics in Oncology and Haematology, 15(3), 303–315.
5. Toh, Y., & Nicolson, G. L. (2013). Signaling pathways of MTA family proteins as regulators of cancer progression and metastasis. In R. R. Resende & H. Ulrich (Eds.), Trends in stem cell proliferation and cancer research (pp. 251–275). Dordrecht: Springer.
6. Manavathi, B., & Kumar, R. (2007). Metastasis tumor antigens, an emerging family of multifaceted master coregulators. Journal of Biological Chemistry, 282(3), 1529–1533.
7. Luo, J., Su, F., Chen, D., Shiloh, A., & Gu, W. (2000). Deacetylation of p53 modulates its effect on cell growth and apoptosis. Nature, 408(6810), 377–381.
8. Moon, H. E., Cheon, H., & Lee, M. S. (2007). Metastasis-associated protein 1 inhibits p53-induced apoptosis. Oncology Reports, 18(5), 1311–1314.
9. Moon, H. E., Cheon, H., Chun, K. H., Lee, S. K., Kim, Y. S., Jung, B. K., et al. (2006). Metastasis-associated protein 1 enhances angiogenesis by stabilization of HIF-1alpha. Oncology Reports, 16(4), 929–935.
10. Yoo, Y. G., Kong, G., & Lee, M. O. (2006). Metastasis-associated protein 1 enhances stability of hypoxia-inducible factor-1alpha protein by recruiting histone deacetylase 1. EMBO Journal, 25(6), 1231–1241.
11. Li, D. Q., Pakala, S. B., Nair, S. S., Eswaran, J., & Kumar, R. (2012). Metastasis-associated protein 1/nucleosome remodeling and histone deacetylase complex in cancer. Cancer Research, 72(2), 387–394.
12. Li, D. Q., & Kumar, R. (2010). Mi-2/NURD complex making inroads into DNA-damage response pathway. Cell Cycle, 9(11), 2071–2079.
13. Pakala, S. B., Reddy, S. D., Bui-Nguyen, T. M., Rangparia, S. S., Bommana, A., & Kumar, R. (2010). MTA1 coregulator regulates LPS response via MyD88-dependent signaling. Journal of Biological Chemistry, 285(43), 32787–32792.
14. Toh, Y., Pencil, S. D., & Nicolson, G. L. (1994). A novel candidate metastasis-associated gene, mta1, differentially expressed in highly metastatic mammary adenocarcinoma cell lines. cDNA cloning, expression, and protein analyses. Journal of Biological Chemistry, 269(37), 22958–22963.
15. Nawa, A., Nishimori, K., Lin, P., Maki, Y., Moue, K., Sawada, H., et al. (2000). Tumor metastasis-associated human MTA1 gene: its deduced protein sequence, localization, and association with breast cancer cell proliferation using antisense phosphorothioate oligonucleotides. Journal of Cell Biochemistry, 79(2), 202–212.
16. Toh, Y., Oki, E., Oda, S., Tokunaga, E., Ohno, S., Maehara, Y., et al. (1997). Overexpression of the MTA1 gene in gastrointestinal carcinomas: correlation with invasion and metastasis. International Journal of Cancer, 74(4), 459–463.
17. Toh, Y., Kuwano, H., Mori, M., Nicolson, G. L., & Sugimachi, K. (1999). Overexpression of metastasis-associated MTA1 mRNA in invasive oesophageal carcinomas. British Journal of Cancer, 79(11–12), 1723–1726.
18. Mazumdar, A., Wang, R. A., Mishra, S. K., Adam, L., Bagheri-Yarmand, R., Mandal, M., et al. (2001). Transcriptional repression of oestrogen receptor by metastasis-associated protein 1 corepressor. Nature Cell Biology, 3(1), 30–37.
19. Toh, Y., Kuninaka, S., Endo, K., Oshiro, T., Ikeda, Y., Nakashima, H., et al. (2000). Molecular analysis of a candidate metastasis-associated gene, MTA1: possible interaction with histone deacetylase 1. Journal of Experimental and Clinical Cancer Research, 19(1), 105–111.
20. Liu, J., Xu, D., Wang, H., Zhang, Y., Chang, Y., Zhang, J., et al. (2014). The subcellular distribution and function of MTA1 in cancer differentiation. Oncotarget, 5(13), 5153–5164.
21. Aramaki, Y., Ogawa, K., Toh, Y., Ito, T., Akimitsu, N., Hamamoto, H., et al. Direct interaction between metastasis-associated protein 1 and endophilin 3. FEBS Letter, 579(17), 3731–3736
22. Paterno, G. D., Li, Y., Luchman, H. A., Ryan, P. J., & Gillespie, L. L. (1997). cDNA cloning of a novel, developmentally regulated immediate early gene activated by fibroblast growth factor and encoding a nuclear protein. Journal of Biological Chemistry, 272(41), 25591–25595.
23. Paterno, G. D., Mercer, F. C., Chayter, J. J., Yan, G. X., Yan, G. X., Robb, J. D., et al. (1998). Molecular cloning of human er1 cDNA and its differential expression in breast tumours and tumour-derived cell lines. Gene, 222(1), 77–82.
24. Herman, M. A., Ch’ng, Q., Hettenbach, S. M., Ratliff, T. M., Kenyon, C., & Herman, R. K. (1999). EGL-27 is similar to a metastasis-associated factor and controls cell polarity and cell migration in C. elegans. Development, 126(5), 1055–1064.
25. Solari, F., Bateman, A., & Ahringer, J. (1999). The Caenorhabditis elegans genes egl-27 and egr-1 are similar to MTA1, a member of a chromatin regulatory complex, and are redundantly required for embryonic patterning. Development, 126(11), 2483–2494.
26. Kleene, R., Zdzieblo, J., Wege, K., & Kern, H. F. (1999). A novel zymogen granule protein (ZG29p) and the nuclear protein MTA1p are differentially expressed by alternative transcription initiation in pancreatic acinar cells of the rat. Journal of Cell Science, 112(Pt15), 2539–2548.
27. Futamura, M., Nishimori, H., Shiratsuchi, T., Saji, S., Nakamura, Y., & Tokino, T. (1999). Molecular cloning, mapping, and characterization of a novel human gene, MTA1-L1, showing homology to a metastasis-associated gene, MTA1. Journal of Human Genetics, 44(1), 52–56.
28. Bowen, N. J., Fujita, N., Kajita, M., & Wade, P. A. (2004). Mi-2/NuRD: multiple complexes for many purposes. Biochimica et Biophysica Acta, 1677(1–3), 52–57.
29. Tong, J. K., Hassig, C. A., Schnitzler, G. R., Kingston, R. E., & Schreiber, S. L. (1998). Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex. Nature, 395(6705), 917–921.
30. Xue, Y., Wong, J., Moreno, G. T., Young, M. K., Cote, J., & Wang, W. (1998). NuRD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities. Molecular Cell, 2(6), 851–861.
31. Zhang, Y., Ng, H. H., Erdjument-Bromage, H., Tempst, P., Bird, A., & Reinberg, D. (1999). Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation. Genes and Development, 13(15), 1924–1935.
32. Wade, P. A., Gegonne, A., Jones, P. L., Ballesta, R. E., Aubry, F., & Wolffe, A. P. (1999). Mi-2 complex couples DNA methylation to chromatin remodelling and histone deacetylation. Nature Genetics, 23(1), 62–66.
33. Fujita, N., Jaye, D. L., Kajita, M., Geigerman, C., Moreno, C. S., & Wade, P. A. (2003). Mta3, a Mi-2/NuRD complex subunit, regulates an invasive growth pathway in breast cancer. Cell, 113(2), 207–219.
34. Basta, J., & Rauchman, M. (2014). The nucleosome remodeling and deacetylase complex in development and disease. Translational Research. doi:10.1016/j.trsl.2014.05.003.
35. Kelly, T. K., De Carvalho, D. D., & Jones, P. A. (2010). Epigenetic modifications as therapeutic targets. Nature Biotechnology, 28(10), 1069–1078.
36. Ramirez, J., & Hagman, J. (2009). The Mi-2/NuRD complex: a critical epigenetic regulator of hematopoietic development, differentiation and cancer. Epigenetics, 4(8), 532–536.
37. Scanlan, M. J., Chen, Y. T., Williamson, B., Gure, A. O., Stockert, E., Gordan, J. D., et al. (1998). Characterization of human colon cancer antigens recognized by autologous antibodies. International Journal of Cancer, 76(5), 652–658.
38. Alqarni, S. S., Murthy, A., Zhang, W., Przewloka, M. R., Silva, A. P., Watson, A. A., et al. (2014). Insight into the architecture of the NuRD complex: structure of the RbAp48-MTA1 subcomplex. Journal of Biological Chemistry, 289(32), 21844–21855.
39. Toh, Y., Ohga, T., Endo, K., Adachi, E., Kusumoto, H., Haraguchi, M., et al. (2004). Expression of the metastasis-associated MTA1 protein and its relationship to deacetylation of the histone H4 in esophageal squamous cell carcinomas. International Journal of Cancer, 110(3), 362–367.
40. Luo, H., Li, H., Yao, N., Hu, L., & He, T. (2014). Metastasis-associated protein 1 as a new prognostic marker for solid tumors: a meta-analysis of cohort studies. Tumour Biology, 35(6), 5823–5832.
41. Nicolson, G. L., Nawa, A., Toh, Y., Taniguchi, S., Nishimori, K., & Moustafa, A. (2003). Tumor metastasis-associated human MTA1 gene and its MTA1 protein product: role in epithelial cancer cell invasion, proliferation and nuclear regulation. Clinical and Experimental Metastasis, 20(1), 19–24.
42. Qian, H., Lu, N., Xue, L., Liang, X., Zhang, X., Fu, M., et al. (2005). Reduced MTA1 expression by RNAi inhibits in vitro invasion and migration of esophageal squamous cell carcinoma cell line. Clinical and Experimental Metastasis, 22(8), 653–662.
43. Qian, H., Yu, J., Li, Y., Wang, H., Song, C., Zhang, X., et al. (2007). RNA interference against metastasis-associated gene 1 inhibited metastasis of B16F10 melanoma cell in c57BL/6 model. Biology of the Cell, 99, 573–581.
44. Singh, R. R., & Kumar, R. (2007). MTA family of transcriptional metaregulators in mammary gland morphogenesis and breast cancer. Journal of Mammary Gland Biology and Neoplasia, 12(2–3), 115–125.
45. Jang, K. S., Paik, S. S., Chung, H., Oh, Y. H., & Kong, G. (2006). MTA1 overexpression correlates significantly with tumor grade and angiogenesis in human breast cancers. Cancer Science, 97(5), 374–379.
46. Martin, M. D., Fischbach, K., Osborne, C. K., Mohsin, S. K., Allred, D. C., & O’Connell, P. (2001). Loss of heterozygosity events impeding breast cancer metastasis contain the mta1 gene. Cancer Research, 61(9), 3578–3580.
47. Martin, M. D., Hilsenbeck, S. G., Mohsin, S. K., Hopp, T. A., Clark, G. M., Osborne, C. K., et al. (2006). Breast tumors that overexpress nuclear metastasis-associated 1 (MTA1) protein have high recurrence risks but enhanced responses to systemic therapies. Breast Cancer Research and Treatment, 95(1), 7–12.
48. Sharma, G., Mirza, S., Parshad, R., Srivastava, A., Gupta, S. D., Pandya, P., et al. (2011). Clinical significance of Maspin promoter methylation and loss of its protein expression in invasive ductal breast carcinoma: correlation with VEGF-A and MTA1 expression. Tumour Biology, 32(1), 23–32.
49. Cheng, C. W., Liu, Y. F., Yu, J. C., Wang, H. W., Ding, S. L., Hsiung, C. N., et al. (2012). Prognostic significance of cyclin D1, beta-catenin, and MTA1 in patients with invasive ductal carcinoma of the breast. Annals of Surgical Oncology, 19(13), 4129–4139.
50. Mao, X. Y., Chen, H., Wang, H., Wei, J., Liu, C., Zheng, H. C., et al. (2012). MTA1 expression correlates significantly with ER-alpha methylation in breast cancer. Tumour Biology, 33(5), 1565–1572.
51. Li, S. H., Wang, Z., & Liu, X. Y. (2009). Metastasis-associated protein 1 (MTA1) overexpression is closely associated with shorter disease-free interval after complete resection of histologically node-negative esophageal cancer. World Journal of Surgery, 33(9), 1876–1881.
52. Li, S. H., Tian, H., Yue, W. M., Li, L., Gao, C., Li, W. J., et al. (2012). Metastasis-associated protein 1 nuclear expression is closely associated with tumor progression and angiogenesis in patients with esophageal squamous cell cancer. World Journal of Surgery, 36(3), 623–631.
53. Song, L., Wang, Z., & Liu, X. (2013). MTA1: a prognosis indicator of postoperative patients with esophageal carcinoma. Thoracic and Cardiovascular Surgery, 61(6), 479–485.
54. Miyatani, T., Kurita, N., Mikami, C., Kashihara, H., Higashijima, J., Yoshikawa, K., et al. (2011). Malignant potential of Barrett’s esophagus: special reference to HDAC-1 and MTA-1 expression. Hepato-Gastroenterology, 58(106), 472–476.
55. Deng, X., Du, L., Wang, C., Yang, Y., Li, J., Liu, H., et al. (2013). Close association of metastasis-associated protein 1 overexpression with increased angiogenesis and poor survival in patients with histologically node-negative gastric cancer. World Journal of Surgery, 37(4), 792–798.
56. Giannini, R., & Cavallini, A. (2005). Expression analysis of a subset of coregulators and three nuclear receptors in human colorectal carcinoma. Anticancer Research, 25(6B), 4287–4292.
57. Du, B., Yang, Z. Y., Zhong, X. Y., Fang, M., Yan, Y. R., Qi, G. L., et al. (2011). Metastasis-associated protein 1 induces VEGF-C and facilitates lymphangiogenesis in colorectal cancer. World Journal of Gastroenterology, 17(9), 1219–1226.
58. Higashijima, J., Kurita, N., Miyatani, T., Yoshikawa, K., Morimoto, S., Nishioka, M., et al. (2011). Expression of histone deacetylase 1 and metastasis-associated protein 1 as prognostic factors in colon cancer. Oncology Reports, 26(2), 343–348.
59. Kidd, M., Modlin, I. M., Mane, S. M., Camp, R. L., Eick, G. N., Latich, I., et al. (2006). Utility of molecular genetic signatures in the delineation of gastric neoplasia. Cancer, 106(7), 1480–1488.
60. Kidd, M., Modlin, I. M., Mane, S. M., Camp, R. L., Eick, G., & Latich, I. (2006). The role of genetic markers—NAP1L1, MAGE-D2, and MTA1—in defining small-intestinal carcinoid neoplasia. Annals of Surgical Oncology, 13(2), 253–262.
61. Modlin, I. M., Kidd, M., Latich, I., Zikusoka, M. N., Eick, G. N., Mane, S. M., et al. (2006). Genetic differentiation of appendiceal tumor malignancy: a guide for the perplexed. Annals of Surgery, 244(1), 52–60.
62. Miyake, K., Yoshizumi, T., Imura, S., Sugimoto, K., Batmunkh, E., Kanemura, H., et al. (2008). Expression of hypoxia-inducible factor-1alpha, histone deacetylase 1, and metastasis-associated protein 1 in pancreatic carcinoma: correlation with poor prognosis with possible regulation. Pancreas, 36(3), e1–e9.
63. Hofer, M. D., Chang, M. C., Hirko, K. A., Rubin, M. A., & Nose, V. (2009). Immunohistochemical and clinicopathological correlation of the metastasis-associated gene 1 (MTA1) expression in benign and malignant pancreatic endocrine tumors. Modern Pathology, 22(7), 933–939.
64. Hamatsu, T., Rikimaru, T., Yamashita, Y., Aishima, S., Tanaka, S., Shirabe, K., et al. (2003). The role of MTA1 gene expression in human hepatocellular carcinoma. Oncology Reports, 10(3), 599–604.
65. Moon, W. S., Chang, K., & Tarnawski, A. S. (2004). Overexpression of metastatic tumor antigen 1 in hepatocellular carcinoma: relationship to vascular invasion and estrogen receptor-alpha. Human Pathology, 35(4), 424–429.
66. Ryu, S. H., Chung, Y. H., Lee, H., Kim, J. A., Shin, H. D., Min, H. J., et al. (2008). Metastatic tumor antigen 1 is closely associated with frequent postoperative recurrence and poor survival in patients with hepatocellular carcinoma. Hepatology, 47(3), 929–936.
67. Lee, S. H., Chung, Y. H., Kim, J. A., Lee, D., Jin, Y. J., Shim, J. H., et al. (2012). Single nucleotide polymorphisms associated with metastatic tumour antigen 1 overexpression in patients with hepatocellular carcinoma. Liver International, 32(3), 457–466.
68. Jin, Y. J., Chung, Y. H., Kim, J. A., Park, W. H., Lee, D., Seo, D. D., et al. (2012). Factors predisposing metastatic tumor antigen 1 overexpression in hepatitis B virus associated hepatocellular carcinoma. Digestive Diseases and Sciences, 57(11), 2917–2923.
69. Roepman, P., de Jager, A., Groot Koerkamp, M. J., Kummer, J. A., Slootweg, P. J., & Holstege, F. C. (2006). Maintenance of head and neck tumor gene expression profiles upon lymph node metastasis. Cancer Research, 66(23), 11110–11114.
70. Kawasaki, G., Yanamoto, S., Yoshitomi, I., Yamada, S., & Mizuno, A. (2008). Overexpression of metastasis-associated MTA1 in oral squamous cell carcinomas: correlation with metastasis and invasion. International Journal of Oral and Maxillofacical Surgery, 37(11), 1039–1046.
71. Li, W. F., Liu, N., Cui, R. X., He, Q. M., Chen, M., Jiang, N., et al. (2012). Nuclear overexpression of metastasis-associated protein 1 correlates significantly with poor survival in nasopharyngeal carcinoma. Journal of Translational Medicine, 10, 78.
72. Deng, Y. F., Zhou, D. N., Ye, C. S., Zeng, L., & Yin, P. (2012). Aberrant expression levels of MTA1 and RECK in nasopharyngeal carcinoma: association with metastasis, recurrence, and prognosis. Annals of Otology, Rhinology and Laryngology, 121(7), 457–465.
73. Park, J. O., Jung, C. K., Sun, D. I., Joo, Y. H., & Kim, M. S. (2011). Relationships between metastasis-associated protein (MTA) 1 and lymphatic metastasis in tonsil cancer. European Archives of Otorhinolaryngology, 268(9), 1329–1334.
74. Sasaki, H., Moriyama, S., Nakashima, Y., Kobayashi, Y., Yukiue, H., Kaji, M., et al. (2002). Expression of the MTA1 mRNA in advanced lung cancer. Lung Cancer, 35(2), 149–154.
75. Zhu, X., Guo, Y., Li, X., Ding, Y., & Chen, L. (2010). Metastasis-associated protein 1 nuclear expression is associated with tumor progression and clinical outcome in patients with non-small cell lung cancer. Journal of Thoracic Oncology, 5(8), 1159–1166.
76. Yu, Y., Wang, Z., Zhang, M. Y., Liu, X. Y., & Zhang, H. (2011). Relation between prognosis and expression of metastasis-associated protein 1 in stage I non-small cell lung cancer. Interactive Cardiovascular and Thoracic Surgery, 12(2), 166–169.
77. Li, S. H., Tian, H., Yue, W. M., Li, L., Li, W. J., Chen, Z. T., et al. (2011). Overexpression of metastasis-associated protein 1 is significantly correlated with tumor angiogenesis and poor survival in patients with early-stage non-small cell lung cancer. Annals of Surgical Oncology, 18(7), 2048–2056.
78. Sasaki, H., Yukiue, H., Kobayashi, Y., Nakashima, Y., Kaji, M., Fukai, I., et al. (2001). Expression of the MTA1 mRNA in thymoma patients. Cancer Letter, 174(2), 159–163.
79. Yi, S., Guangqi, H., & Guoli, H. (2003). The association of the expression of MTA1, nm23H1 with the invasion, metastasis of ovarian carcinoma. Chinese Medical Sciences Journal, 18(2), 87–92.
80. Dannenmann, C., Shabani, N., Friese, K., Jeschke, U., Mylonas, I., & Bruning, A. (2008). The metastasis-associated gene MTA1 is upregulated in advanced ovarian cancer, represses ERbeta, and enhances expression of oncogenic cytokine GRO. Cancer Biology and Therapy, 7(9), 1460–1467.
81. Prisco, M. G., Zannoni, G. F., De Stefano, I., Vellone, V. G., Tortorella, L., Fagotti, A., et al. (2012). Prognostic role of metastasis tumor antigen 1 in patients with ovarian cancer: a clinical study. Human Pathology, 43(2), 282–288.
82. Liu, T., Yang, M., Yang, S., Ge, T., Gu, L., & Lou, G. (2013). Metastasis-associated protein 1 is a novel marker predicting survival and lymph nodes metastasis in cervical cancer. Human Pathology, 44(10), 2275–2281.
83. Hofer, M. D., Kuefer, R., Varambally, S., Li, H., Ma, J., Shapiro, G. I., et al. (2004). The role of metastasis-associated protein 1 in prostate cancer progression. Cancer Research, 64(3), 825–829.
84. Dias, S. J., Zhou, X., Ivanovic, M., Gailey, M. P., Dhar, S., Zhang, L., et al. (2013). Nuclear MTA1 overexpression is associated with aggressive prostate cancer, recurrence and metastasis in African Americans. Scientific Reports, 3, 2331.
85. Hofer, M. D., Tapia, C., Browne, T. J., Mirlacher, M., Sauter, G., & Rubin, M. A. (2006). Comprehensive analysis of the expression of the metastasis-associated gene 1 in human neoplastic tissue. Archives of Pathology and Laboratory Medicine, 130(7), 989–996.