Numerical study of the subtraction threshold for fluorescence difference microscopy

Optics Express

Volumn 22, Issue 23, 2014, Pages 28819-28830

  Wang, Nan ^a,b   Kobayashi, Takayoshi ^a,b,c,d  

^a UNIVERSITY OF ELECTRO COMMUNICATIONS   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c NATIONAL CHIAO TUNG UNIVERSITY   (Taiwan)

^d OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

STRUCTURE (COMPOSITION);

FLUORESCENCE PEAK; NUMERICAL INVESTIGATIONS; RAW IMAGES; SELECTION CRITERIA; SPATIAL RESOLUTION; VECTOR DIFFRACTION THEORY;

FLUORESCENCE;

COMPUTER SIMULATION; FLUORESCENCE MICROSCOPY; IMAGE SUBTRACTION; MATHEMATICAL COMPUTING; PROCEDURES;

COMPUTER SIMULATION; MICROSCOPY, FLUORESCENCE; NUMERICAL ANALYSIS, COMPUTER-ASSISTED; SUBTRACTION TECHNIQUE;

EID: 84913554685     PISSN: None     EISSN: 10944087     Source Type: Journal    
DOI: 10.1364/OE.22.028819     Document Type: Article

References (20)

1. E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. L. Schwartz, and H. F. Hess, "Imaging intracellular fluorescent proteins at nanometer resolution," Science 313, 1642-1645 (2006).
2. M. J. Rust, M. Bates, and X. W. Zhuang, "Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)," Nat. Meth. 3, 793-796 (2006).
3. M. G. L. Gustafsson, "Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy," J. Microscopy 198, 82-87 (2000).
4. S. W. Hell and J. Wichmann, "Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy," Opt. Lett. 19(11), 780-782 (1994).
5. M. P. Gordon, T. Ha, and P. R. Selvin, "Single-molecule high-resolution imaging with photobleaching," P. Natl. Acad. Sci. USA 101(17), 6462-6465 (2004).
6. A. Sharonov and R. M. Hochstrasser, "Wide-field sub-diffraction imaging by accumulated binding of diffusing probes," Proc. Natl. Acad. Sci. USA 103(50), 18911-18916 (2006).
7. T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, "Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI)," P. Natl. Acad. Sci. USA 106(52), 22287-22292 (2009).
8. J. Miyazaki, H. Tsurui, A. Hayashi-Takagi, H. Kasai, and T. Kobayashi, "Sub-diffraction resolution pump-probe microscopy with shot-noise limited sensitivity using laser diodes," Opt. Express 22(8), 9024-9032 (2014).
9. J. Miyazaki, K. Kawasumi, and T. Kobayashi, "Resolution improvement in laser diode-based pump-probe microscopy with an annular pupil filter," Opt. Lett. 39(14), 4219-4222 (2014).
10. C. F. Kuang, S. Li,W. Liu, X. Hao, Z. T. Gu, Y. F.Wang, J. H. Ge, H. F. Li, and X. Liu, "Breaking the diffraction barrier using fluorescence emission difference microscopy," Sci. Rep. 3(1441) (2013).
11. H. Dehez, M. Piche, and Y. D. Koninck, "Resolution and contrast enhancement in laser scanning microscopy using dark beam imaging," Opt. Express 21(13), 15912-15925 (2013).
12. S. Segawa, Y. Kozawa, and S. Sato, "Resolution enhancement of confocal microscopy by subtraction method with vector beams," Opt. Lett. 39(11), 3118-3121 (2014).
13. B. Richards and E. Wolf, "Electromagnetic diffraction in optical systems. II. structure of the image field in an aplanatic system," Proc. R. Soc. Lond. A 253, 358-379 (1959).
14. D. Ganic, X. Gan, and M. Gu, "Focusing of doughnut laser beams by a high numerical-aperture objective in free space," Opt. Express 11(21), 2747-2752 (2003).
15. R. Dorn, S. Quabis, and G. Leuchs, "Sharper focus for a radially polarized light beam," Phys. Rev. Lett. 91, 233901 (2003).
16. G. M. Lerman and U. Levy, "Effect of radial polarization and apodization on spot size under tight focusing conditions," Opt. Express 16(7), 4567-4581 (2008).
17. S. Deng, L. Liu, Y. Cheng, R. Li, and Z. Xu, "Investigation of the influence of the aberration induced by a plane interface on STED microscopy," Opt. Express 17(3), 1714-1725 (2009).
18. X. Hao, C. Kuang, T. Wang, and X. Liu, "Effects of polarization on the de-excitation dark focal spot in STED microscope," J. Opt. 12(115707) (2010).
19. Y. Xue, C. Kuang, S. Li, Z. Gu, and X. Liu, "Sharper fluorescent super-resolution spot generated by azimuthally polarized beam in STED microscopy," Opt. Express 20(16), 17653-17666 (2012).
20. http://en.wikipedia.org/wiki/Circular-polarization.