Breaking the diffraction barrier using fluorescence emission difference microscopy

Scientific Reports

Volumn 3, Issue , 2013, Pages

  Kuang, Cuifang ^a   Li, Shuai ^a   Liu, Wei ^b   Hao, Xiang ^a   Gu, Zhaotai ^a   Wang, Yifan ^a   Ge, Jianhong ^a   Li, Haifeng ^a   Liu, Xu ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84875151211     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep01441     Document Type: Article

References (25)

1. Hell, S. W. Microscopy and its focal switch. Nat. Methods 6, 24-32 (2009).
2. Rust, M. J., Bates, M. & Zhuang, X. W. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat. Methods 3, 793-795 (2006). (Pubitemid 44445825)
3. Betzig, E. et al. Imaging intracellular fluorescent proteins at nanometer resolution. Science 313, 1642-1645 (2006). (Pubitemid 44414039)
4. Hell, S. W. &Wichmann, J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Opt. Lett. 19, 780-782 (1994).
5. Gustafsson, M. G. L. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. J. Microsc. (Oxford, U. K.) 198, 82-87 (2000). (Pubitemid 30316807)
6. Heintzmann, R. & Cremer, C. Laterally modulated excitation microscopy: Improvement of resolution by using a diffraction grating. Proc. SPIE 3568, 185-196 (1999).
7. Carrington,W. A. et al. Superresolution 3-dimensional images of fluorescence in cells with minimal light exposure. Science 268, 1483-1487 (1995).
8. Kano, H., Van Der Voort, H. T. M., Schrader, M., Van Kernpen, G. & Hell, S. W. Avalanche photodiode detection with object scanning and image restoration provides 2-4 fold resolution increase in two-photon fluorescence microscopy. Bioimaging 4, 187-197 (1996). (Pubitemid 27077097)
9. Sentenac, A., Belkebir, K., Giovannini, H.& Chaumet, P. C. High-resolution totalinternal-reflection fluorescence microscopy using periodically nanostructured glass slides. J. Opt. Soc. Am. A 26, 2550-2557 (2009).
10. Gustafsson, M. G. L. Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution. Proc. Natl. Acad. Sci. U. S. A. 102, 13081-13086 (2005). (Pubitemid 41324982)
11. Westphal, V. & Hell, S. W. Nanoscale resolution in the focal plane of an optical microscope. Phys. Rev. Lett. 94, 143903 (2005).
12. Xu, K., Babcock, H. P. & Zhuang, X. W. Dual-objective STORM reveals threedimensional filament organization in the actin cytoskeleton. Nat. Methods 9, 185-188 (2012).
13. Brodehl, A. et al. Dual color photoactivation localization microscopy of cardiomyopathy-associated desmin mutants. J. Biol. Chem. 287, 16047-16057 (2012).
14. Cox, S. et al. Bayesian localization microscopy reveals nanoscale podosome dynamics. Nat. Methods 9, 195-200 (2012).
15. Zhu, L., Zhang, W., Elnatan, D. & Huang, B. Faster STORM using compressed sensing. Nat. Methods 9, 721-723 (2012).
16. Engelhardt, J. et al. Molecular orientation affects localization accuracy in superresolution far-field fluorescence microscopy. Nano Lett. 11, 209-213 (2011).
17. Heintzmann, R., Jovin, T. M. & Cremer, C. Saturated patterned excitation microscopy-a concept for optical resolution improvement. J. Opt. Soc. Am. A 19, 1599-1609 (2002).
18. Wilson, T.&Hamilton, D. K. Difference confocal scanningmicroscopy. Opt. Acta 31, 453-465 (1984).
19. Heintzmann, R. et al. Resolution enhancement by subtraction of confocal signals taken at different pinhole sizes. Micron 34, 293-300 (2003). (Pubitemid 37110747)
20. Barroca, T., Balaa, K., Lévêque-Fort, S. & Fort, E. Full-field near-field optical microscope for cell imaging. Phys. Rev. Lett. 108, 218101 (2012).
21. Hewlett, S. &Wilson, T. Resolution enhancement in three-dimensional confocal microscopy. Mach. Vis. Appl. 4, 233-242 (1991).
22. Wilson, T. Resolution and optical sectioning in the confocal microscope. J. Microsc. (Oxford, U. K.) 244, 113-121 (2011).
23. Fujita, K.,Kobayashi,M., Kawano, S., Yamanaka, M.&Kawata, S.High-resolution confocal microscopy by saturated excitation of fluorescence. Phys. Rev. Lett. 99, 228105 (2007).
24. Born, M. &Wolf, E. Principles of Optics: Electromagnetic Theory of Propagation, Interference and Diffraction of light, 6th ed. (corr.). (Cambridge University Press, Cambridge, UK; New York: 1997).
25. Mudry, E. et al. Structured illumination microscopy using unknown speckle patterns. Nat. Photonics 6, 312-315 (2012).