Nitroreductase-activatable morpholino oligonucleotides for in vivo gene silencing

ACS Chemical Biology

Volumn 9, Issue 9, 2014, Pages 1985-1990

  Yamazoe, Sayumi ^a   McQuade, Lindsey E ^a   Chen, James K ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; MORPHOLINO OLIGONUCLEOTIDE; NITROREDUCTASE; NITROREDUCTASE NFSB; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; ESCHERICHIA COLI PROTEIN; NFSB PROTEIN, E COLI;

ARTICLE; CATALYST; CELL POPULATION; EMBRYO; GENE SILENCING; HOMEOBOX; IN VITRO STUDY; IN VIVO STUDY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOTONEURON; NONHUMAN; PHENOTYPE; PROTEIN EXPRESSION; WESTERN BLOTTING; ZEBRA FISH; ANIMAL; ANIMAL EMBRYO; CHEMISTRY; DRUG EFFECTS; EMBRYOLOGY; FEMALE; GENETICS; HEK293 CELL LINE; HUMAN; LIGHT; METABOLISM; SYNTHESIS; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CHEMISTRY TECHNIQUES, SYNTHETIC; EMBRYO, NONMAMMALIAN; ESCHERICHIA COLI PROTEINS; FEMALE; GENE SILENCING; HEK293 CELLS; HUMANS; LIGHT; MORPHOLINOS; NITROREDUCTASES; ZEBRAFISH;

EID: 84913546475     PISSN: 15548929     EISSN: 15548937     Source Type: Journal    
DOI: 10.1021/cb500429u     Document Type: Article

References (32)

1. Shestopalov, I. A., and Chen, J. K. (2010) Oligonucleotide-based tools for studying zebrafish development. Zebrafish 7, 31-40.
2. Summerton, J., and Weller, D. (1997) Morpholino antisense oligomers: design, preparation, and properties. Antisense Nucleic Acid Drug Dev. 7, 187-195.
3. Heasman, J., Kofron, M., and Wylie, C. (2000) Beta-catenin signaling activity dissected in the early Xenopus embryo: a novel antisense approach. Dev. Biol. 222, 124-134.
4. Nasevicius, A., and Ekker, S. C. (2000) Effective targeted gene 'knockdown' in zebrafish. Nat. Genet. 26, 216-220.
5. Kos, R., Reedy, M. V., Johnson, R. L., and Erickson, C. A. (2001) The winged-helix transcription factor FoxD3 is important for establishing the neural crest lineage and repressing melanogenesis in avian embryos. Development 128, 1467-1479.
6. Howard, E. W., Newman, L. A., Oleksyn, D. W., Angerer, R. C., and Angerer, L. M. (2001) SpKrl: a direct target of beta-catenin regulation required for endoderm differentiation in sea urchin embryos. Development 128, 365-375.
7. Shestopalov, I. A., Sinha, S., and Chen, J. K. (2007) Light-ontrolled gene silencing in zebrafish embryos. Nat. Chem. Biol. 3, 650-651.
8. Tomasini, A. J., Schuler, A. D., Zebala, J. A., and Mayer, A. N. (2009) PhotoMorphs: a novel light-activated reagent for controlling gene expression in zebrafish. Genesis 47, 736-743.
9. Tallafuss, A., Gibson, D., Morcos, P., Li, Y., Seredick, S., Eisen, J., and Washbourne, P. (2012) Turning gene function ON and OFF using sense and antisense photo-morpholinos in zebrafish. Development 139, 1691-1699.
10. Deiters, A., Garner, R. A., Lusic, H., Govan, J. M., Dush, M., Nascone-Yoder, N. M., and Yoder, J. A. (2010) Photocaged morpholino oligomers for the light-regulation of gene function in zebrafish and Xenopus embryos. J. Am. Chem. Soc. 132, 15644-15650.
11. Yamazoe, S., Shestopalov, I. A., Provost, E., Leach, S. D., and Chen, J. K. (2012) Cyclic caged morpholinos: conformationally gated probes of embryonic gene function. Angew. Chem., Int. Ed. 51, 6908-6911.
12. Wang, Y., Wu, L., Wang, P., Lv, C., Yang, Z., and Tang, X. (2012) Manipulation of gene expression in zebrafish using caged circular morpholino oligomers. Nucleic Acids Res. 40, 11155-11162.
13. Shestopalov, I. A., Pitt, C. L., and Chen, J. K. (2012) Spatiotemporal resolution of the Ntla transcriptome in axial mesoderm development. Nat. Chem. Biol. 8, 270-276.
14. Moore, J. C., Sheppard-Tindell, S., Shestopalov, I. A., Yamazoe, S., Chen, J. K., and Lawson, N. D. (2013) Post-transcriptional mechanisms contribute to Etv2 repression during vascular development. Dev. Biol. 384, 128-140.
15. Zenno, S., Koike, H., Tanokura, M., and Saigo, K. (1996) Gene cloning, purification, and characterization of NfsB, a minor oxygeninsensitive nitroreductase from Escherichia coli, similar in biochemical properties to FRase I, the major flavin reductase in Vibrio fischeri. J. Biochem. 120, 736-744.
16. Clark, A. J., Iwobi, M., Cui, W., Crompton, M., Harold, G., Hobbs, S., Kamalati, T., Knox, R., Neil, C., Yull, F., and Gusterson, B. (1997) Selective cell ablation in transgenic mice expression E. coli nitroreductase. Gene Ther. 4, 101-110.
17. Kwak, S. P., Malberg, J. E., Howland, D. S., Cheng, K. Y., Su, J., She, Y., Fennell, M., and Ghavami, A. (2007) Ablation of central nervous system progenitor cells in transgenic rats using bacterial nitroreductase system. J. Neurosci. Res. 85, 1183-1193.
18. Pisharath, H., Rhee, J. M., Swanson, M. A., Leach, S. D., and Parsons, M. J. (2007) Targeted ablation of beta cells in the embryonic zebrafish pancreas using E. coli nitroreductase. Mech. Dev. 124, 218-229.
19. Curado, S., Anderson, R. M., Jungblut, B., Mumm, J., Schroeter, E., and Stainier, D. Y. (2007) Conditional targeted cell ablation in zebrafish: a new tool for regeneration studies. Dev. Dyn. 236, 1025-1035.
20. Halpern, M. E., Ho, R. K., Walker, C., and Kimmel, C. B. (1993) Induction of muscle pioneers and floor plate is distinguished by the zebrafish no tail mutation. Cell 75, 99-111.
21. Schulte-Merker, S., van Eeden, F. J., Halpern, M. E., Kimmel, C. B., and Nusslein-Volhard, C. (1994) no tail (ntl) is the zebrafish homologue of the mouse T (Brachyury) gene. Development 120, 1009-1015.
22. Ouyang, X., Shestopalov, I. A., Sinha, S., Zheng, G., Pitt, C. L., Li, W. H., Olson, A. J., and Chen, J. K. (2009) Versatile synthesis and rational design of caged morpholinos. J. Am. Chem. Soc. 131, 13255-13269.
23. Biemar, F., Argenton, F., Schmidtke, R., Epperlein, S., Peers, B., and Driever, W. (2001) Pancreas development in zebrafish: early dispersed appearance of endocrine hormone expressing cells and their convergence to form the definitive islet. Dev. Biol. 230, 189-203.
24. Field, H. A., Dong, P. D., Beis, D., and Stainier, D. Y. (2003) Formation of the digestive system in zebrafish. II. Pancreas morphogenesis. Dev. Biol. 261, 197-208.
25. Hesselson, D., Anderson, R. M., Beinat, M., and Stainier, D. Y. (2009) Distinct populations of quiescent and proliferative pancreatic beta-cells identified by HOTcre mediated labeling. Proc. Natl. Acad. Sci. U.S.A. 106, 14896-14901.
26. Kimmel, R. A., Onder, L., Wilfinger, A., Ellertsdottir, E., and Meyer, D. (2011) Requirement for Pdx1 in specification of latent endocrine progenitors in zebrafish. BMC Biol. 9, 75.
27. Dalgin, G., Ward, A. B., Hao le, T., Beattie, C. E., Nechiporuk, A., and Prince, V. E. (2011) Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas. Development 138, 4597-4608.
28. Wendik, B., Maier, E., and Meyer, D. (2004) Zebrafish mnx genes in endocrine and exocrine pancreas formation. Dev. Biol. 268, 372-383.
29. Mathias, J. R., Zhang, Z., Saxena, M. T., and Mumm, J. S. (2014) Enhanced cell-specific ablation in zebrafish using a triple mutant of Escherichia coli nitroreductase. Zebrafish 11, 85-97.
30. Thisse, C., and Thisse, B. (2008) High-resolution in situ hybridization to whole-mount zebrafish embryos. Nat. Protoc. 3, 59-69.
31. Milewski, W. M., Duguay, S. J., Chan, S. J., and Steiner, D. F. (1998) Conservation of PDX-1 structure, function, and expression in zebrafish. Endocrinology 139, 1440-1449.
32. Griffin, K. J., and Kimelman, D. (2003) Interplay between FGF, one-eyed pinhead, and T-box transcription factors during zebrafish posterior development. Dev. Biol. 264, 456-466.