Spectrally resolved fluorescence lifetime imaging to investigate cell metabolism in malignant and nonmalignant oral mucosa cells

Journal of Biomedical Optics

Volumn 19, Issue 9, 2014, Pages

  Rück, Angelika ^a   Hauser, Carmen ^a   Mosch, Simone ^a   Kalinina, Sviatlana ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

2p microscopy; Autofluorescence; Cell metabolism; Hybrid detector; Nicotinamide adenine dinucleotide; Redox ratio; Spectrally resolved fluorescence lifetime imaging

Indexed keywords

CELLS; DIAGNOSIS; FLUORESCENCE; METABOLISM; PHYSIOLOGY; REDOX REACTIONS;

AUTOFLUORESCENCE; CELL METABOLISM; DINUCLEOTIDES; HYBRID DETECTORS; REDOX-RATIO; RESOLVED FLUORESCENCE;

CYTOLOGY;

NICOTINAMIDE ADENINE DINUCLEOTIDE;

CHEMISTRY; CYTOLOGY; FLUORESCENCE IMAGING; HUMAN; IMAGE PROCESSING; METABOLISM; MOUTH MUCOSA; MOUTH TUMOR; PROCEDURES; TUMOR CELL LINE;

CELL LINE, TUMOR; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MOUTH MUCOSA; MOUTH NEOPLASMS; NAD; OPTICAL IMAGING;

EID: 84911488499     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.JBO.19.9.096005     Document Type: Article

References (47)

1. R. A. Gatenby and R. J. Gillies, "Why do cancers have high aerobic glycolysis?," Nat. Rev. 4(11), 891-899 (2004
2. O. Warburg, "On the origin of cancer cells," Science 123(3191), 309-314 (1956
3. S. S. Gambhir, "Molecular imaging of cancer with positron emission tomography," Nat. Rev. Cancer 2(9), 683-693 (2002
4. A. Periasamy and R. M. Clegg, FLIM Microscopy in Biology and Medicine, A. Periasamy and R. M. Clegg, Eds., Taylor & Francis Group, Boca Raton, FL (2009
5. B. Chance et al., "Oxidation-Reduction ratio studies of mitochondria in freeze-trapped samples," J. Biol. Chem. 254(11), 4764-4771 (1979
6. M. C. Skala et al., "In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia," PNAS 104(49), 19494-19499 (2007
7. J. R. Lakowicz et al., "Fluorescence lifetime imaging of free and protein-bound NADH," PNAS 89(4), 1271-1275 (1992
8. Y. Wu, W. Zheng, and J. Y. Qu, "Sensing cell metabolism by timeresolved autofluorescence," Opt. Lett. 31(21), 3122-3124 (2006
9. C. Stringari et al., "Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in a live tissue," PNAS 108(33), 13582-13587 (2011
10. K. König and H. Schneckenburger, "Laser-induced autofluorescence for medical diagnosis," J. Fluoresc. 4(1), 17-40 (1994
11. J. R. Lakowicz, Principles of Fluorescence Spectroscopy, 2nd ed., Kluwer/Academic Plenum Publishers, New York (1999
12. R. Huber et al., "Protein binding of NADH on chemical preconditioning," J. Neurochem. 75(1), 329-335 (2000
13. N. L. Vekshin, Photonics of Biopolymers, Springer, Berlin (2002
14. D. Li, W. Zheng, and J. Y. Qu, "Time-resolved spectroscopic imaging reveals the fundamentals of cellular NADH fluorescence," Opt. Lett. 33(20), 2365-2367 (2008
15. Q. Zhang, D.W. Piston, and R. H. Goodman, "Regulation of corepressor function by nuclear NADH," Science 295(5561), 1895-1897 (2002
16. C. C. Fjeld, W. T. Birdsong, and R. H. Goodman, "Differential binding of NAD+ and NADH allows the transcriptional corepressor carboxylterminal binding protein to serve as a metabolic sensor," PNAS, 100(16), 9202-9207 (2003
17. K. König, A. Uchugonova, and E. Gorjup, "Multiphoton fluorescence lifetime imaging of 3D-stem cell spheroids during differentiation," Microsc. Res. Tech. 74(1), 9-17 (2011
18. M. A. Yaseen et al., "In vivo imaging of cerebral energy metabolism with two-photon fluorescence lifetime microscopy of NADH," Biomed. Opt. Express 4(2), 307-321 (2013
19. M. C. Skala et al., "In vivo multiphoton fluorescence lifetime imaging of protein-bound and free NADH in normal and pre-cancerous epithelia," J. Biomed. Opt. 12(2), 024014 (2007
20. D. K. Bird et al., "Metabolic mapping of MCF10A human breast cells via multiphoton fluorescence lifetime imaging of the coenzyme NADH," Cancer Res. 65(19), 8766-8773 (2005
21. C. Stringari et al., "Metabolic trajectory of cellular differentiation in small intestine by phasor fluorescence lifetime microscopy of NADH," Sci. Rep. 2, 1-9 (2012
22. Y. Sun et al., "Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery," J. Biomed. Opt. 15(5), 056022 (2010
23. J. Leppert et al., "Multiphoton excitation of autofluorescence for microscopy of glioma tissue," Neurosurgery 58(4), 759-767 (2006
24. J. McGinty et al., "Wide-field fluorescence lifetime imaging of cancer," Biomed. Opt. Express 1(2), 627-640 (2010
25. A. J. Walsh et al., "Optical metabolic imaging identifies glycolytic levels, subtypes, and early-treatment response in breast cancer," Cancer Res. 73(20), 6164-6174 (2013
26. A. Rück et al., "SLIM: a new method for molecular imaging," Micro Res. Technol. 70(5), 485-492 (2007
27. C. Biskup et al., "Spectrally resolved fluorescence lifetime imaging microscopy: SLIM/mwFLIM," in FLIM Microscopy in Biology and Medicine, A. Periasami and R. M. Clegg, Eds., pp. 211-244, Taylor & Francis Group, Boca Raton, FL (2009
28. W. Becker, A. Bergmann, and C. Biskup, "Multispectral fluorescence lifetime imaging by TCSPC," Microsc. Res. Tech. 70(5), 403-409 (2007
29. M. A. Dickinson et al., "Human keratinocytes that express hTERT and also bypass a p16(INK4a)-enforced mechanism that limits life span become immortal yet retain normal growth and differentiation characteristics," Mol. Cell Biol. 20(4), 1436-1447 (2000
30. J. G. Rheinwald and M. A. Beckett, "Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas," Cancer Res. 41(5), 1657-1663 (1981
31. M. Agostini et al., "Fatty acid synthase is required for the proliferation of human oral squamous carcinoma cells," Oral Oncol. 40(7), 728-735 (2004
32. E. Gratton, "Globals Software SimFCS 3.0," Laboratory for Fluorescence Dynamics, University of California, Irvine, http://www.lfd.uci.edu/globals/(2006-2014
33. J. H. Ostrander et al., "Optical redox ratio differentiates breast cancer cell lines based on estrogen receptor status," Cancer Res. 70(11), 4759-4766 (2010
34. A. Walsh et al., "Optical imaging of metabolism in HER2 overexpressing breast cancer cells," Biomed. Opt. Express 3(1), 75-85 (2012
35. M. C. Skala et al., "Multiphoton microscopy of endogenous fluorescence differentiates normal, precancerous, and cancerous squamous epithelial tissues," Cancer Res. 65(4), 1180-1186 (2005
36. K. Edward et al., "In vivo layer-resolved characterization of oral dysplasia via nonlinear optical micro-spectroscopy," Biomed. Opt. Express 3(7), 1579-1593 (2012
37. N. Ramanujam et al., "In vivo diagnosis of cervical intraepithelial neoplasia using 337-nm-excited laser-induced fluorescence," Proc. Natl. Acad. Sci. U. S. A. 91(21), 10193-10197 (1994
38. J. Lakowicz, Principles of Fluorescence Spectroscopy, Plenum Publishers, New York (1999
39. H. W. Guo et al., "Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies," J. Biomed. Opt. 13(5), 050505 (2008
40. A. Uppal and P. K. Gupta, "Measurement of NADH concentration in normal and malignant human tissues from breast and oral cavity," Biotechnol. Appl. Biochem. 37(1), 45-50 (2003
41. A. Chorvatova et al., "Effect of ouabain on metabolic oxidative state in living cardiomyocytes evaluated by time-resolved spectroscopy of endogenous NAD(P)H fluorescence," J. Biomed. Opt. 17(10), 101505 (2012
42. K. König et al., "Two-photon excited lifetime imaging of autofluorescence in cells during UVA and NIR photostress," J. Microsc. 183(Pt 3), 197-204 (1996
43. A. Chorvatova et al., "Time-resolved fluorescence spectroscopy investigation of the effect of 4-hydroxynonenal on endogenous NAD(P)H in living cardiac myocytes," J. Biomed. Opt. 18(6), 067009 (2013
44. V. K. Ramanujan, "Metabolic imaging in multiple time scales," Methods 66(2), 222-229 (2014
45. D. Strat et al., "Spectrally resolved fluorescence lifetime imaging: FRET Global Analysis with a one-and two-exponential donor model," J. Biomed. Opt. 16(2), 026002 (2011
46. M. A. Digman et al., "The phasor approach to fluorescence lifetime imaging analysis," Biophys. J. 94(2), 14-16 (2008
47. F. Fereidouni, A. N. Bader, and H. C. Gerritsen, "Spectral phasor analysis allows rapid and reliable unmixing of fluorescence microscopy spectral images," Opt. Express 20(12), 12729-12741 (2012).