Multispectral fluorescence lifetime imaging by TCSPC

Microscopy Research and Technique

Volumn 70, Issue 5, 2007, Pages 403-409

  Becker, Wolfgang ^a   Bergmann, Axel ^a   Biskup, Christoph ^b  

^a BECKER AND HICKL GMBH   (Germany)

^b FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

Author keywords

Autofluorescence; FLIM; FRET; Multispectral FLIM; TCSPC

Indexed keywords

FLUORESCENCE IMAGING; FORSTER RESONANCE ENERGY TRANSFER; PARTICLE BEAMS; SPECTROGRAPHS;

AUTOFLUORESCENCE; FIBRE BUNDLE; FLIM; FLUORESCENCE LIFETIME IMAGING; FULLY COMPATIBLE; MULTI-SPECTRAL; MULTIPHOTONS; MULTISPECTRAL FLIM; TEMPORAL RESOLUTION; TIME-CORRELATED SINGLE PHOTON COUNTING;

PHOTONS;

AUTOFLUORESCENCE; CONFERENCE PAPER; CORRELATION ANALYSIS; EXCITATION; FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; IMAGING; LENS; MULTIPHOTON MICROSCOPY; PRIORITY JOURNAL; SOUND DETECTION; SPECTRAL SENSITIVITY;

EID: 34249095092     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20432     Document Type: Conference Paper

References (43)

1. Bacskai BJ, Skoch J, Hickey GA, Allen R, Hyman BT. 2003. Fluorescence resonance energy transfer determinations using multiphoton fluorescence lifetime imaging microscopy to characterize amyloid-β plaques. J Biomed Opt 8:368-375.
2. Becker W, Bergmann A, König K, Tirlapur U. 2001. Picosecond fluorescence lifetime microscopy by TCSPC imaging. Proc SPIE 4262:414-419.
3. Becker W, Bergmann A, Biskup C, Zimmer T, Klöcker N, Benndorf K. 2002. Multi-wavelength TCSPC lifetime imaging. Proc SPIE 4620:79-84.
4. Becker W, Bergmann A, Hink MA, König K, Benndorf K, Biskup C. 2004. Fluorescence lifetime imaging by time-correlated single photon counting. Micr Res Techn 63:58-66.
5. Becker W. 2005a. Advanced time-correlated single-photon counting techniques. Berlin: Springer.
6. Becker W. 2005b. The bh TCSPC handbook. Available at www.becker-hickl.com
7. Bird DK, Eliceiri KW, Fan C-H, White JG. 2004. Simultaneous two-photon spectral and lifetime fluorescence microscopy. Appl Opt 43:5173-5182.
8. Biskup C, Kelbauskas L, Zimmer T, Benndorf K, Bergmann A, Becker W, Ruppersberg JP, Stockklausner C, Klöcker N. 2004a. Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging. J Biomed Opt 9:735-759.
9. + channel subunits measured with a confocal microscope and a streak camera. Nature Biotech 22:220-224.
10. Biskup C, Böhmer A, Pusch R, Kelbauskas L, Gorshkov A, Majoul I, Lindenau J, Benndorf K, Böhmer F-D. 2004c. Visualization of SHP-1-target interaction. J Cell Sci. 117:5155-5178.
11. Biskup C, Kelbauskas L, Hoffmann B, Zimmer T, Klöcker N, Becker W, Bermann A, Benndorf K. Multidimensional fluorescence lifetime and FRET measurements. Micr Res Techn (in press).
12. Brismar H, Ulfhake B. 1997. Fluorescence lifetime imaging measurements in confocal microscopy of neurons labeled with multiple fluorophores. Nature Biotech 15:373-377.
13. Bugiel I, König K, Wabnitz H. 1989. Investigations of cells by fluorescence laser scanning microscopy with subnanosecond time resolution. Lasers Life Sci 3:47-53.
14. Denk W, Strickler JH, Webb WW. 1990. Two-photon laser scanning fluorescence microscopy. Science 24:73-76.
15. Calleja V, Ameer-Beg S, Vojnovic B, Woscholski R, Downwards J, Larijani B. 2003. Monitoring conformational changes of proteins in cells by fluorescence lifetime imaging microscopy. Biochem J 372:33-40.
16. Diaspro A, editors. 2001. Confocal and two-photon microscopy: Foundations, applications and advances. New York: Wiley-Liss.
17. Dickinson ME, Waters CW, Bearman G, Wolleschensky R, Tille S, Fraser SE. 2002. Sensitive imaging of spectrally overlapping fluorochromes using the LSM 510 META. Proc SPIE 4620:123-136.
18. Duncan RR, Bergmann A, Cousin MA, Apps DK, Shipston MJ. 2004. Multi-dimensional time-correlated single-photon counting (TCSPC) fluorescence lifetime imaging microscopy (FLIM) to detect FRET in cells. J Microsc 215:1-12.
19. Gerritsen HC, Sanders R, Draaijer A, Levine YK. 1997. Fluorescence lifetime imaging of oxygen in cells. J Fluoresc 7:11-16.
20. Hanson KM, Behne MJ, Barry NP, Mauro TM, Gratton E. 2002. Two-photon fluorescence imaging of the skin stratum corneum pH gradient. Biophys J 83:1682-1690.
21. Hopt A, Neher E. 2002. Highly nonlinear photodamage in two-photon fluorescence microscopy. Biophys J 80:2029-2036.
22. Kaneko H, Putzier I, Frings S, Kaupp UB, Gensch Th. 2004. Chloride accumulation in mammalian olfactory sensory neurons. J Neurosci 24:7931-7938.
23. Kelbauskas L, Dietel W. 2002. Internalization of aggregated photosensitizers by tumor cells: Subcellular time-resolved fluorescence spectroscopy on derivates of pyropheophorbide-a ethers and chlorin e6 under femtosecond one- and two-photon excitation. Photochem Photobiol 76:686-694.
24. König K, So PTC, Mantulin WW, Tromberg BJ, Gratton E. 1996. Two-Photon excited lifetime imaging of autofluorescence in cells during UVA and NIR photostress. J Microsc 183:197-204.
25. König K, Multiphoton microscopy in life sciences. 2000. J Microsc 200:83-104.
26. König K, Riemann I. 2003. High resolution optical tomography of human skin with subcellular resolution and picosecond time resolution. J Biomed Opt 8:432-439.
27. Lakowicz JR, Szmacinski H, Nowaczyk K, Johnson ML. 1992. Fluorescence lifetime imaging of free and protein-bound NADH. PNAS 89:1271-1275.
28. 2+, and glucose. Sens Actuator Chem 11:133-134.
29. Lakowicz JR. 1999. Principles of fluorescence spectroscopy, 2nd ed. New York: Kluwer Academic.
30. Liebert A, Wabnitz H, Grosenick D, Macdonald R. 2003. Fiber dispersion in time domain measurements compromising the accuracy of determination of optical properties of strongly scattering media. J Biomed Opt 8:512-516.
31. O'Connor DV, Phillips D. 1984. Time correlated single photon counting. London: Academic Press.
32. Patterson GH, Piston DW. 2000. Photobleaching in two-photon excitation microscopy. Biophys J 78:2159-2162.
33. Pawley J, editor. 1995. Handbook of biological confocal microscopy. New York: Plenum Press.
34. Peter M, Ameer-Beg SM. 2004. Imaging molecular interactions by multiphoton FLIM. Biol Cell 96:231-236.
35. Peter M, Ameer-Beg SM, Hughes MKY, Keppler MD, Prag S, Marsh M, Vojnovic B, Ng T. 2005. Multiphoton-FLIM quantification of the EGFP-mRFP1 FRET pair for localization of membrane receptor-kinase interactions. Biophys J 88:1224-1237.
36. Rück A, Dolp F, Hülshoff C, Hauser C, Scalfi-Happ C. 2005. Fluorescence lifetime imaging in PDT An overview. Med Laser Appl 20:125-129.
37. Schweitzer D, Hammer M, Schweitzer F, Anders R, Doebbecke T, Schenke S, Gaillard ER. 2004. In vivo measurement of time-resolved autofluorescence at the human fundus. J Biomed Opt 9:1214-1222.
38. Schweitzer D, Hammer M, Schweitzer F, Schenke S, Birckner E, Becker W, Axel Bergmann A. 2006. In vivo autofluorescence life-time imaging at the fundus of the human eye. Proc SPIE 6138.
39. Thaler C, Koushik SV, Blank PS, Vogel SS. 2005. Quantitative multiphoton spectral imaging and its use for measuring resonance energy transfer. Biophys J 89:2736-2749.
40. Treanor B, Lanigan PMP, Suhling K, Schreiber T, Munro I, Neil MAA, Phillips D, Davis DM, French PMW. 2005. Imaging fluorescence lifetime heterogeneity applied to GFP-tagged MHC protein at an immunological synapse. J Microsc 217:36-43.
41. White JG, Amos WB, Fordham M. 1987. An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy. J Cell Biol 105:41-48.
42. Van Zandvoort MAMJ, de Grauw CJ, Gerritsen HC, Broers JLV, Egbrink MGA, Ramaekers FCS, Slaaf DW. 2002. Discrimination of DNA and RNA in cells by a vital fluorescent probe: Lifetime imaging of SYTO13 in healthy and apoptotic cells. Cytometry 47:226-223.
43. Yguerabide J, Nanosecond fluorescence spectroscopy of macromolecules. 1972. Meth Enzymol 26:498-578.