Isolating potentiated hsp104 variants using yeast proteinopathy models

Journal of Visualized Experiments

Volumn , Issue 93, 2014, Pages

  Jackrel, Meredith E ^a   Tariq, Amber ^a   Yee, Keolamau ^a   Weitzman, Rachel ^a   Shorter, James ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Amyloid; Disaggregation; Hsp104; Issue 93; Microbiology; Protein misfolding disorders; Proteotoxicity; Yeast proteinopathy models

Indexed keywords

EID: 84911458202     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/52089     Document Type: Article

References (24)

1. Johnson, B. S., McCaffery, J. M., Lindquist, S., Gitler, A. D. A yeast TDP-43 proteinopathy model: Exploring the molecular determinants of TDP-43 aggregation and cellular toxicity. Proc Natl Acad Sci USA.105, (17), 6439-6444 (2008).
2. Sun, Z., et al. Molecular Determinants and Genetic Modifiers of Aggregation and Toxicity for the ALS Disease Protein FUS/TLS. PLoS Biol. 9, (4), (2011).
3. Outeiro, T. F., Lindquist, S. Yeast Cells Provide Insight into Alpha-Synuclein Biology and Pathobiology. Science. 302, (5651), 1772-1775 (2003).
4. Forman, M. S., Trojanowski, J. Q., Lee, V. M. Neurodegenerative diseases: a decade of discoveries paves the way for therapeutic breakthroughs. Nat Med. 10, (10), 1055-1063 (2004).
5. Morimoto, R. I. Stress, aging, and neurodegenerative disease. N Engl J Med. 355, (21), 2254-2255 (2006).
6. Elden, A. C., et al. Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Nature. 466, (7310), 1069-1075 (2010).
7. Cooper, A. A., et al. Alpha-synuclein blocks ER-Golgi traffic and Rab1 rescues neuron loss in Parkinson's models. Science. 313, (5785), 324-328 (2006).
8. Gitler, A. D., et al. Alpha-synuclein is part of a diverse and highly conserved interaction network that includes PARK9 and manganese toxicity. Nature genetics. 41, (3), 308-315 (2009).
9. Su, L. J., et al. Compounds from an unbiased chemical screen reverse both ER-to-Golgi trafficking defects and mitochondrial dysfunction in Parkinson's disease models. Disease models & mechanisms. (3-4), 194-208 (2010).
10. Tardiff, D. F., et al. Yeast reveal a 'druggable' Rsp5/Nedd4 network that ameliorates alpha-synuclein toxicity in neurons. Science. 342, (6161), 979-983 (2013).
11. Tardiff, D. F., Tucci, M. L., Caldwell, K. A., Caldwell, G. A., Lindquist, S. Different 8-hydroxyquinolines protect models of TDP-43 protein, alpha-synuclein, and polyglutamine proteotoxicity through distinct mechanisms. The Journal of biological chemistry. 287, (6), 4107-4120 (2012).
12. Kim, H. J., et al. Therapeutic modulation of eIF2alpha phosphorylation rescues TDP-43 toxicity in amyotrophic lateral sclerosis disease models. Nature. 46, (2), 152-160 (2014).
13. Ju, S., et al. A yeast model of FUS/TLS-dependent cytotoxicity. PLoS Biol. 9, (4), (2011).
14. Shorter, J. Hsp104: a weapon to combat diverse neurodegenerative disorders. Neurosignals. 16, (1), 63-74 (2008).
15. Vashist, S., Cushman, M., Shorter, J. Applying Hsp104 to protein-misfolding disorders. Biochem Cell Biol.88, (1), 1-13 (2010).
16. DeSantis, M. E., et al. Operational Plasticity Enables Hsp104 to Disaggregate Diverse Amyloid and Nonamyloid Clients. Cell. 151, (4), 778-793 (2012).
17. Jackrel, M. E., et al. Potentiated Hsp104 Variants Antagonize Diverse Proteotoxic Misfolding Events. Cell.156, (1-2), 170-182 (2014).
18. Wittrup, K. D., Verdine, G. L. Methods in Enzymology. Wittrup, K. D., Verdine, G. L. 503, 13-14 Academic Press (2012).
19. Boeke, J. D., Trueheart, J., Natsoulis, G., Fink, G. R. Methods in Enzymology. 154, 164-175 Academic Press Lawrence Grossman Ray Wu (1987).
20. Miyazaki, K. Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP). Directed Evolution Library Creation: Methods in Molecular Biology. eds FrancesH Arnold & George Georgiou. 231, 23-28 Humana Press (2003).
21. Saibil, H. Chaperone machines for protein folding, unfolding and disaggregation. Nat Rev Mol Cell Biol.14, (10), 630-642 (2013).
22. Gietz, R. D., Schiestl, R. H. High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat. Protocols. 2, (1), 31-34 (2007).
23. Armakola, M., Hart, M. P., Gitler, A. D. TDP-43 toxicity in yeast. Methods. 53, (3), 238-245 (2011).
24. Alberti, S., Gitler, A. D., Lindquist, S. A suite of Gateway® cloning vectors for high-throughput genetic analysis in Saccharomyces cerevisiae. Yeast. 24, (10), 913-919 (2007).