Antibody-free magnetic cell sorting of genetically modified primary human CD4+ T cells by one-step streptavidin affinity purification

PLoS ONE

Volumn 9, Issue 10, 2014, Pages

  Matheson, Nicholas J ^a   Peden, Andrew A ^b   Lehner, Paul J ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN; CARRIER PROTEINS AND BINDING PROTEINS; SHORT HAIRPIN RNA; STREPTAVIDIN BINDING PEPTIDE; UNCLASSIFIED DRUG; AFFINITY LABELING; CARRIER PROTEIN; SMALL INTERFERING RNA; STREPTAVIDIN-BINDING PEPTIDE;

AFFINITY PURIFICATION; ANTIBODY FREE MAGNETIC CELL SORTING; ANTIGEN ANTIBODY COMPLEX; ARTICLE; BINDING AFFINITY; BINDING COMPETITION; CD4+ T LYMPHOCYTE; CELL ISOLATION; CELL SELECTION; CELL SURFACE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONJUGATION; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; ENRICHMENT CULTURE; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LYMPHOCYTE SUBPOPULATION; PROCESS OPTIMIZATION; PROTEIN BINDING; PROTEIN PURIFICATION; AFFINITY LABELING; CELL SEPARATION; CRISPR CAS SYSTEM; CYTOLOGY; GENETICS; HEK293 CELL LINE; MAGNETISM; METABOLISM; PROCEDURES; TIME FACTOR;

AFFINITY LABELS; CARRIER PROTEINS; CD4-POSITIVE T-LYMPHOCYTES; CELL SEPARATION; CRISPR-CAS SYSTEMS; HEK293 CELLS; HUMANS; MAGNETIC PHENOMENA; RNA, SMALL INTERFERING; TIME FACTORS;

EID: 84909998713     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0111437     Document Type: Article

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