Chronic hypoxia increases trpc6 expression and basal intracellular Ca2+ concentration in rat distal pulmonary venous smooth muscle

PLoS ONE

Volumn 9, Issue 11, 2014, Pages

  Xu, Lei ^a,b   Chen, Yuqin ^a   Yang, Kai ^a,b   Wang, Yingfeng ^a   Tian, Lichun ^a   Zhang, Jie ^a   Wang, Elizabeth Wenqian ^d   Sun, Dejun ^c   Lu, Wenju ^a,b   Wang, Jian ^a,b,c  

^a GUANGZHOU MEDICAL UNIVERSITY   (China)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c INNER MONGOLIA PEOPLE S HOSPITAL   (China)

^d GEISINGER MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ION; FURA 2; MESSENGER RNA; TRANSIENT RECEPTOR POTENTIAL CHANNEL 6; CALCIUM; TRANSIENT RECEPTOR POTENTIAL CHANNEL C; TRPC6 PROTEIN, RAT;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CELL CULTURE; CELL ISOLATION; CELL MIGRATION; CELL PROLIFERATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; HYPOXIA; IN VITRO STUDY; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PULMONARY HYPERTENSION; PULMONARY VEIN; PULMONARY VENOUS SMOOTH MUSCLE CELL; QUANTITATIVE ANALYSIS; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SMOOTH MUSCLE FIBER; WESTERN BLOTTING; ANIMAL; ANOXIA; BIOSYNTHESIS; CALCIUM SIGNALING; CHRONIC DISEASE; GENE EXPRESSION REGULATION; METABOLISM; PATHOLOGY; SPRAGUE DAWLEY RAT; VASCULAR REMODELING; VASCULAR SMOOTH MUSCLE;

RATTUS;

ANIMALS; ANOXIA; CALCIUM; CALCIUM SIGNALING; CHRONIC DISEASE; GENE EXPRESSION REGULATION; HYPERTENSION, PULMONARY; MALE; MUSCLE, SMOOTH, VASCULAR; PULMONARY VEINS; RATS; RATS, SPRAGUE-DAWLEY; TRPC CATION CHANNELS; VASCULAR REMODELING;

EID: 84909953908     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0112007     Document Type: Article

References (39)

1. Stenmark KR, Fagan KA, Frid MG (2006) Hypoxia-induced pulmonary vascular remodeling: cellular and molecular mechanisms. Circ Res 99: 675-691.
2. Kuhr FK, Smith KA, Song MY, Levitan I, Yuan JX (2012) New mechanisms of pulmonary arterial hypertension: role of Ca(2+ ) signaling. Am J Physiol Heart Circ Physiol 302: H1546-1562.
3. Wang J, Shimoda LA, Sylvester JT (2004) Capacitative calcium entry and TRPC channel proteins are expressed in rat distal pulmonary arterial smooth muscle. Am J Physiol Lung Cell Mol Physiol 286: L848-858.
4. Ambudkar IS, Ong HL (2007) Organization and function of TRPC channelosomes. Pflugers Arch 455: 187-200.
5. Zhu X, Chu PB, Peyton M, Birnbaumer L (1995) Molecular cloning of a widely expressed human homologue for the Drosophila trp gene. FEBS Lett 373: 193-198.
6. Lu W, Wang J, Shimoda LA, Sylvester JT (2008) Differences in STIM1 and TRPC expression in proximal and distal pulmonary arterial smooth muscle are associated with differences in Ca2+ responses to hypoxia. Am J Physiol Lung Cell Mol Physiol 295: L104-113.
7. Wang J, Weigand L, Lu W, Sylvester JT, Semenza GL, et al. (2006) Hypoxia inducible factor 1 mediates hypoxia-induced TRPC expression and elevated intracellular Ca2+ in pulmonary arterial smooth muscle cells. Circ Res 98: 1528-1537.
8. Gao Y, Raj JU (2005) Role of veins in regulation of pulmonary circulation. Am J Physiol Lung Cell Mol Physiol 288: L213-226.
9. Hillier SC, Graham JA, Hanger CC, Godbey PS, Glenny RW, et al. (1997) Hypoxic vasoconstriction in pulmonary arterioles and venules. J Appl Physiol 82: 1084-1090.
10. Zhao Y, Packer CS, Rhoades RA (1993) Pulmonary vein contracts in response to hypoxia. Am J Physiol 265: L87-92.
11. Raj JU, Hillyard R, Kaapa P, Gropper M, Anderson J (1990) Pulmonary arterial and venous constriction during hypoxia in 3-to 5-wk-old and adult ferrets. J Appl Physiol 69: 2183-2189.
12. Dingemans KP, Wagenvoort CA (1978) Pulmonary arteries and veins in experimental hypoxia. An ultrastructural study. Am J Pathol 93: 353-368.
13. Finnegan P (1977) The pulmonary circulation in chronic bronchitis and emphysema. Ir J Med Sci 146: 233-238.
14. Migally N, Tucker A, Zambernard J (1982) Fine structural changes in pulmonary veins of chronically hypoxic mice. J Submicrosc Cytol 14: 239-246.
15. Takahashi H, Soma S, Muramatsu M, Oka M, Fukuchi Y (2001) Upregulation of ET-1 and its receptors and remodeling in small pulmonary veins under hypoxic conditions. Am J Physiol Lung Cell Mol Physiol 280: L1104-1114.
16. Peng G, Lu W, Li X, Chen Y, Zhong N, et al. (2010) Expression of storeoperated Ca2+ entry and transient receptor potential canonical and vanilloidrelated proteins in rat distal pulmonary venous smooth muscle. Am J Physiol Lung Cell Mol Physiol 299: L621-630.
17. Peng G, Ran P, Lu W, Zhong N, Wang J (2013)Acute hypoxia activates storeoperated Ca(2+ ) entry and increases intracellular Ca(2+ ) concentration in rat distal pulmonary venous smooth muscle cells. J Thorac Dis 5: 605-612.
18. Peng G, Wang J, Lu W, Ran P (2010) Isolation and primary culture of rat distal pulmonary venous smooth muscle cells. Hypertens Res 33: 308-313.
19. Lu W, Ran P, Zhang D, Peng G, Li B, et al. (2010) Sildenafil inhibits chronically hypoxic upregulation of canonical transient receptor potential expression in rat pulmonary arterial smooth muscle. Am J Physiol Cell Physiol 298: C114-123.
20. Zhang Y, Lu W, Yang K, Xu L, Lai N, et al. (2013) Bone morphogenetic protein 2 decreases TRPC expression, store-operated Ca(2+ ) entry, and basal [Ca(2+ )]i in rat distal pulmonary arterial smooth muscle cells. Am J Physiol Cell Physiol 304: C833-843.
21. Wang J, Yang K, Xu L, Zhang Y, Lai N, et al. (2013) Sildenafil Inhibits Hypoxia-Induced Transient Receptor Potential Canonical Protein Expression in Pulmonary Arterial Smooth Muscle via cGMP-PKG-PPARgamma Axis. Am J Respir Cell Mol Biol 49: 231-240.
22. Michelakis ED, Weir EK, Wu X, Nsair A, Waite R, et al. (2001) Potassium channels regulate tone in rat pulmonary veins. Am J Physiol Lung Cell Mol Physiol 280: L1138-1147.
23. Chazova I, Loyd JE, Zhdanov VS, Newman JH, Belenkov Y, et al. (1995) Pulmonary artery adventitial changes and venous involvement in primary pulmonary hypertension. Am J Pathol 146: 389-397.
24. Wang J, Jiang Q, Wan L, Yang K, Zhang Y, et al. (2013) Sodium tanshinone IIA sulfonate inhibits canonical transient receptor potential expression in pulmonary arterial smooth muscle from pulmonary hypertensive rats. Am J-Respir Cell Mol Biol 48: 125-134.
25. Boulay G (2002) Ca(2+ )-calmodulin regulates receptor-operated Ca(2+ ) entry activity of TRPC6 in HEK-293 cells. Cell Calcium 32: 201-207.
26. Hassock SR, Zhu MX, Trost C, Flockerzi V, Authi KS (2002) Expression and role of TRPC proteins in human platelets: evidence that TRPC6 forms the storeindependent calcium entry channel. Blood 100: 2801-2811.
27. El Boustany C, Bidaux G, Enfissi A, Delcourt P, Prevarskaya N, et al. (2008) Capacitative calcium entry and transient receptor potential canonical 6 expression control human hepatoma cell proliferation. Hepatology 47: 2068-2077.
28. Yu Y, Sweeney M, Zhang S, Platoshyn O, Landsberg J, et al. (2003) PDGF stimulates pulmonary vascular smooth muscle cell proliferation by upregulating TRPC6 expression. Am J Physiol Cell Physiol 284: C316-330.
29. Ramanathan G, Gupta S, Thielmann I, Pleines I, Varga-Szabo D, et al. (2012) Defective diacylglycerol-induced Ca2+ entry but normal agonist-induced activation responses in TRPC6-deficient mouse platelets. J Thromb Haemost 10: 419-429.
30. Kunichika N, Landsberg JW, Yu Y, Kunichika H, Thistlethwaite PA, et al. (2004) Bosentan inhibits transient receptor potential channel expression in pulmonary vascular myocytes. Am J Respir Crit Care Med 170: 1101-1107.
31. Yu Y, Fantozzi I, Remillard CV, Landsberg JW, Kunichika N, et al. (2004) Enhanced expression of transient receptor potential channels in idiopathic pulmonary arterial hypertension. Proc Natl Acad Sci U S A 101: 13861-13866.
32. Ng LC, O'Neill KG, French D, Airey JA, Singer CA, et al. (2012) TRPC1 and Orai1 interact with STIM1 and mediate capacitative Ca(2+ ) entry caused by acute hypoxia in mouse pulmonary arterial smooth muscle cells. Am J Physiol Cell Physiol 303: C1156-1172.
33. Wang C, Li JF, Zhao L, Liu J, Wan J, et al. (2009) Inhibition of SOC/Ca2+ / NFAT pathway is involved in the anti-proliferative effect of sildenafil on pulmonary artery smooth muscle cells. Respir Res 10: 123.
34. Cheng KT, Ong HL, Liu X, Ambudkar IS (2011) Contribution of TRPC1 and Orai1 to Ca(2+ ) entry activated by store depletion. Adv Exp Med Biol 704: 435-449.
35. Yang Q, Lu Z, Ramchandran R, Longo LD, Raj JU (2012) Pulmonary artery smooth muscle cell proliferation and migration in fetal lambs acclimatized to high-altitude long-term hypoxia: role of histone acetylation. Am J Physiol Lung Cell Mol Physiol 303: L1001-1010.
36. Yu L, Hales CA (2011) Silencing of sodium-hydrogen exchanger 1 attenuates the proliferation, hypertrophy, and migration of pulmonary artery smooth muscle cells via E2F1. Am J Respir Cell Mol Biol 45: 923-930.
37. Leggett K, Maylor J, Undem C, Lai N, Lu W, et al. (2012) Hypoxia-induced migration in pulmonary arterial smooth muscle cells requires calcium-dependent upregulation of aquaporin 1. Am J Physiol Lung Cell Mol Physiol 303: L343-353.
38. Remillard CV, Yuan JX (2006) TRP channels, CCE, and the pulmonary vascular smooth muscle. Microcirculation 13: 671-692.
39. Zhang S, Yuan JX, Barrett KE, Dong H (2005) Role of Na + /Ca2+ exchange in regulating cytosolic Ca2+ in cultured human pulmonary artery smooth muscle cells. Am J Physiol Cell Physiol 288: C245-252.