Sildenafil inhibits chronically hypoxic upregulation of canonical transient receptor potential expression in rat pulmonary arterial smooth muscle

American Journal of Physiology - Cell Physiology

Volumn 298, Issue 1, 2010, Pages

  Lu, Wenju ^a,b   Ran, Pixin ^a   Zhang, Dandan ^a   Peng, Gongyong ^a,b   Li, Bing ^a   Zhong, Nanshan ^a   Wang, Jian ^a,b  

^a GUANGZHOU MEDICAL UNIVERSITY   (China)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Basal intracellular calcium; Calcium signaling; Store operated Ca2+ entry

Indexed keywords

CALCIUM CHANNEL; CALCIUM ION; MESSENGER RNA; SILDENAFIL; SMALL INTERFERING RNA; STORE OPERATED CALCIUM ION ENTRY PROTEIN; TRANSIENT RECEPTOR POTENTIAL CHANNEL 1; TRANSIENT RECEPTOR POTENTIAL CHANNEL 6; TRANSIENT RECEPTOR POTENTIAL CHANNEL C; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BASEMENT MEMBRANE; CONTROLLED STUDY; CROSS LINKING; DISEASE COURSE; DISEASE SEVERITY; DRUG INHIBITION; DRUG MECHANISM; EX VIVO STUDY; HEART RIGHT VENTRICLE HYPERTROPHY; HEART RIGHT VENTRICLE PRESSURE; HYPOXIA; IN VIVO STUDY; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PULMONARY ARTERY; PULMONARY HYPERTENSION; RAT; RECEPTOR DOWN REGULATION; RECEPTOR UPREGULATION; SMOOTH MUSCLE; SMOOTH MUSCLE FIBER;

ANIMALS; BLOOD PRESSURE; CALCIUM; CELL HYPOXIA; GENE EXPRESSION REGULATION; HYPERTROPHY, RIGHT VENTRICULAR; MALE; MUSCLE, SMOOTH, VASCULAR; PIPERAZINES; PULMONARY ARTERY; PURINES; RATS; RATS, WISTAR; RNA, MESSENGER; RNA, SMALL INTERFERING; SULFONES; TRPC CATION CHANNELS; UP-REGULATION; VASODILATOR AGENTS; VENTRICULAR FUNCTION, RIGHT;

RATTUS;

EID: 73649120970     PISSN: 03636143     EISSN: 15221563     Source Type: Journal    
DOI: 10.1152/ajpcell.00629.2008     Document Type: Article

References (41)

1. Abbott D, Comby P, Charuel C, Graepel P, Hanton G, Leblanc B, Lodola A, Longeart L, Paulus G, Peters C, Stadler J. Preclinical safety profile of sildenafil. Int J Impot Res 16: 498-504, 2004.
2. Andersen CU, Mulvany MJ, Simonsen U. Lack of synergistic effect of molsidomine and sildenafil on development of pulmonary hypertension in chronic hypoxic rats. Eur J Pharmacol 510: 87-96, 2005.
3. Barnett CF, Machado RF. Sildenafil in the treatment of pulmonary hypertension. Vasc Health Risk Manag 2: 411-422, 2006.
4. Beech DJ. Emerging functions of 10 types of TRP cationic channel in vascular smooth muscle. Clin Exp Pharmacol Physiol 32: 597-603, 2005.
5. Berbey C, Allard B. Electrically silent divalent cation entries in resting and active voltage-controlled muscle fibers. Biophys J 96: 2648-2657, 2009.
6. 2+ entry by depolarization of skeletal myotubes. Proc Natl Acad Sci USA 101: 15793-15798, 2004.
7. Cornwell TL, Pryzwansky KB, Wyatt TA, Lincoln TM. Regulation of sarcoplasmic reticulum protein phosphorylation by localized cyclic GMP-dependent protein kinase in vascular smooth muscle cells. Mol Pharmacol 40: 923-931, 1991.
8. Dietrich A, Kalwa H, Fuchs B, Grimminger F, Weissmann N, Gudermann T. In vivo TRPC functions in the cardiopulmonary vasculature. Cell Calcium 42: 233-244, 2007.
9. El Boustany C, Bidaux G, Enfissi A, Delcourt P, Prevarskaya N, Capiod T. Capacitative calcium entry and transient receptor potential canonical 6 expression control human hepatoma cell proliferation. Hepatology 47: 2068-2077, 2008.
10. Guilluy C, Sauzeau V, Rolli-Derkinderen M, Guerin P, Sagan C, Pacaud P, Loirand G. Inhibition of RhoA/Rho kinase pathway is involved in the beneficial effect of sildenafil on pulmonary hypertension. Br J Pharmacol 146: 1010-1018, 2005.
11. Karaki H, Ozaki H, Hori M, Mitsui-Saito M, Amano K, Harada K, Miyamoto S, Nakazawa H, Won KJ, Sato K. Calcium movements, distribution, and functions in smooth muscle. Pharmacol Rev 49: 157-230, 1997.
12. Komalavilas P, Penn RB, Flynn CR, Thresher J, Lopes LB, Furnish EJ, Guo M, Pallero MA, Murphy-Ullrich JE, Brophy CM. The small heat shock-related protein, HSP20, is a cAMP-dependent protein kinase substrate that is involved in airway smooth muscle relaxation. Am J Physiol Lung Cell Mol Physiol 294: L69-L78, 2008.
13. Kunichika N, Landsberg JW, Yu Y, Kunichika H, Thistlethwaite PA, Rubin LJ, Yuan JX. Bosentan inhibits transient receptor potential channel expression in pulmonary vascular myocytes. Am J Respir Crit Care Med 170: 1101-1107, 2004.
14. Kwan HY, Huang Y, Yao X. Regulation of canonical transient receptor potential isoform 3 (TRPC3) channel by protein kinase G. Proc Natl Acad Sci USA 101: 2625-2630, 2004.
15. 2+ channels in pulmonary arterial smooth muscle cells: a novel mechanism of hypoxic pulmonary hypertension. Circ Res 95: 496-505, 2004.
16. 2+ responses to hypoxia. Am J Physiol Lung Cell Mol Physiol 295: L104-L113, 2008.
17. Michelakis ED, Tymchak W, Noga M, Webster L, Wu XC, Lien D, Wang SH, Modry D, Archer SL. Long-term treatment with oral sildenafil is safe and improves functional capacity and hemodynamics in patients with pulmonary arterial hypertension. Circulation 108: 2066-2069, 2003.
18. Osinski MT, Rauch BH, Schror K. Antimitogenic actions of organic nitrates are potentiated by sildenafil and mediated via activation of protein kinase A. Mol Pharmacol 59: 1044-1050, 2001.
19. Parekh AB, Penner R. Store depletion and calcium influx. Physiol Rev 77: 901-930, 1997.
20. Parekh AB, Putney JW Jr. Store-operated calcium channels. Physiol Rev 85: 757-810, 2005.
21. Pauvert O, Bonnet S, Rousseau E, Marthan R, Savineau JP. Sildenafil alters calcium signaling and vascular tone in pulmonary arteries from chronically hypoxic rats. Am J Physiol Lung Cell Mol Physiol 287: L577-L583, 2004.
22. Pauvert O, Lugnier C, Keravis T, Marthan R, Rousseau E, Savineau JP. Effect of sildenafil on cyclic nucleotide phosphodiesterase activity, vascular tone and calcium signaling in rat pulmonary artery. Br J Pharmacol 139: 513-522, 2003.
23. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: e45, 2001.
24. Remillard CV, Yuan JX. TRP channels, CCE, and the pulmonary vascular smooth muscle. Microcirculation 13: 671-692, 2006.
25. Sebkhi A, Strange JW, Phillips SC, Wharton J, Wilkins MR. Phosphodiesterase type 5 as a target for the treatment of hypoxia-induced pulmonary hypertension. Circulation 107: 3230-3235, 2003.
26. Shimoda LA, Semenza GL. Functional analysis of the role of hypoxia-inducible factor 1 in the pathogenesis of hypoxic pulmonary hypertension. Methods Enzymol 381: 121-129, 2004.
27. i, and ET-1-induced responses in chronically hypoxic pulmonary myocytes. Am J Physiol Lung Cell Mol Physiol 279: L884-L894, 2000.
28. 2+ channels and chronic hypoxia. Microcirculation 13: 657-670, 2006.
29. 2+ entry and attenuates pulmonary artery smooth muscle cell proliferation. Am J Physiol Lung Cell Mol Physiol 283: L144-L155, 2002.
30. Takahashi A, Camacho P, Lechleiter JD, Herman B. Measurement of intracellular calcium. Physiol Rev 79: 1089-1125, 1999.
31. Tantini B, Manes A, Fiumana E, Pignatti C, Guarnieri C, Zannoli R, Branzi A, Galie N. Antiproliferative effect of sildenafil on human pulmonary artery smooth muscle cells. Basic Res Cardiol 100: 131-138, 2005.
32. Wang J, Shimoda LA, Sylvester JT. Capacitative calcium entry and TRPC channel proteins are expressed in rat distal pulmonary arterial smooth muscle. Am J Physiol Lung Cell Mol Physiol 286: L848-L858, 2004.
33. 2+ in pulmonary arterial smooth muscle cells. Circ Res 98: 1528-1537, 2006.
34. v channel gene expression in rat distal pulmonary artery. Am J Physiol Lung Cell Mol Physiol 288: L1049-L1058, 2005.
35. 2+ entry in endothelial cells. Am J Physiol Heart Circ Physiol 277: H1498-H1504, 1999.
36. 2+ channels in mouse mesangial cells. Am J Physiol Cell Physiol 287: C357-C364, 2004.
37. + channel expression in pulmonary arterial myocytes. Am J Physiol Lung Cell Mol Physiol 294: L309-L318, 2008.
38. 2+. Handb Exp Pharmacol: 527-540, 2007.
39. Yu Y, Fantozzi I, Remillard CV, Landsberg JW, Kunichika N, Platoshyn O, Tigno DD, Thistlethwaite PA, Rubin LJ, Yuan JX. Enhanced expression of transient receptor potential channels in idiopathic pulmonary arterial hypertension. Proc Natl Acad Sci USA 101: 13861-13866, 2004.
40. Yu Y, Sweeney M, Zhang S, Platoshyn O, Landsberg J, Rothman A, Yuan JX. PDGF stimulates pulmonary vascular smooth muscle cell proliferation by upregulating TRPC6 expression. Am J Physiol Cell Physiol 284: C316-C330, 2003.
41. Zhao L, Mason NA, Morrell NW, Kojonazarov B, Sadykov A, Maripov A, Mirrakhimov MM, Aldashev A, Wilkins MR. Sildenafil inhibits hypoxia-induced pulmonary hypertension. Circulation 104: 424-428, 2001.