Comparative characterization of mAb producing hapten-specific hybridoma cells by flow cytometric analysis and ELISA

Journal of Immunological Methods

Volumn 413, Issue , 2014, Pages 45-56

  Kuhne, Maren ^a   Dippong, Martin ^a,b   Flemig, Sabine ^a   Hoffmann, Katrin ^a   Petsch, Kristin ^a   Schenk, Jörg A ^b,c   Kunte, Hans Jörg ^a   Schneider, Rudolf J ^a  

^a BAM FEDERAL INSTITUTE FOR MATERIALS RESEARCH AND TESTING   (Germany)

^b UNIVERSITY OF POTSDAM   (Germany)

^c Hybrotec GmbH   (Germany)

Author keywords

ELISA; Flow cytometry; Hapten; Hybridoma; Immunization; Monoclonal antibodies

Indexed keywords

AFLATOXIN B1; DIGOXIGENIN; ESTRADIOL; ESTRONE; HAPTEN; IMMUNOGLOBULIN G; IMMUNOGLOBULIN G ANTIBODY; MONOCLONAL ANTIBODY; ZEARALENONE; ANTIGEN; BOVINE SERUM ALBUMIN;

ANIMAL CELL; ANTIBODY AFFINITY; ANTIBODY BLOOD LEVEL; ANTIBODY PRODUCTION; ANTIBODY RESPONSE; ANTIBODY SPECIFICITY; ANTIGEN SPECIFICITY; ARTICLE; BINDING AFFINITY; CELL CLONE; CELL SURFACE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; FLOW CYTOMETRY; FLUORESCENCE; HIGH THROUGHPUT SCREENING; HYBRIDOMA CELL CULTURE; HYBRIDOMA CELL LINE; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOGLOBULIN PRODUCING CELL; IMMUNOGLOBULIN PRODUCTION; IMMUNOHISTOCHEMISTRY; MOUSE; NONHUMAN; ANIMAL; BAGG ALBINO MOUSE; BIOSYNTHESIS; CHEMISTRY; COMPARATIVE STUDY; HYBRIDOMA; IMMUNOLOGY; PROCEDURES;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; ANTIBODY-PRODUCING CELLS; ANTIGENS; CLONE CELLS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESTRONE; FEMALE; FLOW CYTOMETRY; HAPTENS; HIGH-THROUGHPUT SCREENING ASSAYS; HYBRIDOMAS; IMMUNOGLOBULIN G; IMMUNOHISTOCHEMISTRY; MICE; MICE, INBRED BALB C; SERUM ALBUMIN, BOVINE;

EID: 84909586870     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2014.07.004     Document Type: Article

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