Comparison of hybridoma screening methods for the efficient detection of high-affinity hapten-specific monoclonal antibodies

Journal of Immunological Methods

Volumn 329, Issue 1-2, 2008, Pages 184-193

  Cervino, Christian ^a   Weber, Ekkehard ^b   Knopp, Dietmar ^a   Niessner, Reinhard ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Aflatoxin; ELISA; Hapten specific; Hybridoma screening; Monoclonal antibody; Mycotoxin

Indexed keywords

AFLATOXIN; HAPTEN; IMMUNOGLOBULIN G ANTIBODY; MONOCLONAL ANTIBODY;

ANIMAL EXPERIMENT; ANTIBODY AFFINITY; ANTIBODY DETECTION; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HYBRIDOMA; IC 50; INTERMETHOD COMPARISON; MOUSE; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SUPERNATANT;

AFLATOXINS; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; BINDING SITES, ANTIBODY; BINDING, COMPETITIVE; CELL LINE; CELLS, CULTURED; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HAPTENS; HYBRIDOMAS; MICE; MICE, INBRED BALB C; REPRODUCIBILITY OF RESULTS;

EID: 36849001257     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2007.10.010     Document Type: Article

References (26)

1. Abad A., and Montoya A. Production of monoclonal antibodies for carbaryl from a hapten preserving the carbamate group. J. Agric. Food Chem. 42 (1994) 1818
2. Berglund G.I., Carlsson G.H., Smith A.T., Szöke H., Henriksen A., and Hajdu J. The catalytic pathway of horseradish peroxidase at high resolution. Nature 417 (2002) 463
3. Burrin J., and Newman D.J. Production and assessment of antibodies. In: Price C.P., and Newman D.J. (Eds). Principles and Practice of Immunoassay (1991), Stockton Press, New York 19
4. Canziani G.A., Klakamp S., and Myszka D.G. Kinetic screening of antibodies from crude hybridoma samples using Biacore. Anal. Biochem. 325 (2004) 301
5. Cervino C., Knopp D., Weller M.G., and Niessner R. Novel aflatoxin derivatives and protein conjugates. Molecules 12 (2007) 641
6. Cho Y.-J., Lee D.-H., Kim D.-O., Min W.-K., Bong K.-T., Lee G.-G., and Seo J.-H. Production of a monoclonal antibody against ochratoxin A and its application to immunochromatographic assay. J. Agric. Food Chem. 53 (2005) 8447
7. Daigo K., Sugita S., Mochizuki Y., Iwanari H., Hiraishi K., Miyano K., Kodama T., and Hamakubo T. A simple hybridoma screening method for high-affinity monoclonal antibodies using the signal ratio obtained from time-resolved fluorescence assay. Anal. Biochem. 351 (2006) 219
8. In: Eaton D.L., and Groopman J.D. (Eds). The Toxicology of Aflatoxins. Human Health, Veterinary, and Agricultural Significance (1994), Academic Press, San Diego
9. Food and Agriculture Organization, 2004. Worldwide regulations for mycotoxins in food and feed 2003. FAO Food and Nutr. Paper 81.
10. Grol M., and Schulze M. Immunoassays für lösliche Antigene: Ein Überblick. In: Peters J.H., and Baumgarten H. (Eds). Monoklonale Antikörper (1990), Springer, Berlin/Heidelberg 321
11. Hack R., Märtlbauer E., and Terplan G. A monoclonal antibody to the trichotecene T-2 toxin: screening for the antibody by a direct enzyme immunoassay. J. Vet. Med. 34 (1987) 538
12. 2 oximinoacetate. J. Agric. Food Chem. 36 (1989) 404
13. Kane M.M., and Banks J.N. Raising antibodies. In: Gosling J.P. (Ed). Immunoassays - a Practical Approach (2000), Oxford University Press, Oxford 19
14. Köhler G., and Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256 (1975) 495
15. Manclús J.J., Abad A., Lebedev M.Y., Mojarrad F., Micková B., Mercader J.V., Primo J., Miranda M.A., and Montoya A. Development of a monoclonal immunoassay selective for chlorinated cyclodiene insecticides. J. Agric. Food Chem. 52 (2004) 2776
16. Mangler J., Weller M.G., Weil L., Niessner R., Hämmerle H., and Schlosshauer B. New monoclonal antibodies to triazine herbicides. Fresenius J. Anal. Chem. 349 (1994) 346
17. Matschulat D., Deng A., Niessner R., and Knopp D. Development of a highly sensitive monoclonal antibody based ELISA for detection of benzo[a]pyrene in potable water. Analyst 130 (2005) 1078
18. Mercader J.V., and Montoya A. Development of monoclonal ELISAs for azinphos-methyl. 1. Hapten synthesis and antibody production. J. Agric. Food Chem. 47 (1999) 1276
19. Moreno M.J., Abad A., and Montoya A. Production of monoclonal antibodies to the N-methylcarbamate pesticide propoxur. J. Agric. Food Chem. 49 (2001) 72
20. 2 in acetonitrile, methanol, and toluene-acetonitrile (9 + 1) (modification of AOAC official method 971.22): collaborative study. J. Assoc. Off. Anal. Chem. Int. 82 (1999) 251
21. Plückthun A., and Pack P. New protein engineering approaches to multivalent and bispecific antibody fragments. Immunotechnology 3 (1997) 83
22. Roitt I.M., and Delves P.J. Roitt's Essential Immunology (2006), Blackwell Publishing, Malden, MA
23. Sasaki K., Glass T.R., and Ohmura N. Validation of accuracy of enzyme-linked immunosorbent assay in hybridoma screening and proposal of an improved screening method. Anal. Chem. 77 (2005) 1933
24. Schetters H. Screening. In: Masseyeff R.F., Albert W.H., and Staines N.A. (Eds). Methods of Immunological Analysis, Vol. 2. (1993), VCH, Weinheim 246
25. Weller, M.G., 1992. Strukturelle und kinetische Untersuchungen zur Entwicklung und Optimierung von Hapten-Enzymimmunoassays (ELISAs) am Beispiel der Bestimmung von Triazinherbiziden. Dissertation, Technische Universität München.
26. Winklmair M., Weller M.G., Mangler J., Schlosshauer B., and Niessner R. Development of a highly sensitive enzyme-immunoassay for the determination of triazine herbicides. Fresenius J. Anal. Chem. 358 (1997) 614