A stable and highly sensitive 3,3´,5,5´-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays

Journal of Immunological Methods

Volumn 233, Issue 1-2, 2000, Pages 47-56

  Frey, Andreas ^a   Meckelein, Barbara ^a   Externest, Dörthe ^a   Schmidt, M Alexander ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

3,3 ,5,5 Tetramethylbenzidine; Horseradish peroxidase; Immunoassay; Sensitivity; Substrate reagent

Indexed keywords

BUFFER; HORSERADISH PEROXIDASE; HYDROGEN PEROXIDE; IMMUNOGLOBULIN A; IMMUNOGLOBULIN A ANTIBODY; IMMUNOGLOBULIN G; IMMUNOGLOBULIN G ANTIBODY; REAGENT; TETRAMETHYLBENZIDINE;

ARTICLE; ASSAY; BLOOD SAMPLING; COLOR; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME SUBSTRATE; FEMALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE;

ANIMALS; BENZIDINES; CHROMOGENIC COMPOUNDS; DRUG STABILITY; ENZYME-LINKED IMMUNOSORBENT ASSAY; EVALUATION STUDIES; FEMALE; HORSERADISH PEROXIDASE; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; INDICATORS AND REAGENTS; MICE; MICE, INBRED BALB C; OVALBUMIN; SENSITIVITY AND SPECIFICITY; SUBSTRATE SPECIFICITY;

EID: 0033955085     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00166-0     Document Type: Article

References (15)

1. Bos E.S., van der Doelen A.A., van Rooy N., Schuurs A.H.W.M. 3,3′,5,5′-Tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay. J. Immunoassay. 2:1981;187.
2. Cattaneo M.V., Luong J.H.T. A stable water-soluble tetramethylbenzidine-2-hydroxypropyl-β-cyclodextrin inclusion complex and its application in enzyme assays. Anal. Biochem. 223:1994;313.
3. Crowther J.R., Angarita L., Anderson J. Evaluation of the use of chromogenic and fluorogenic substrates in solid-phase enzyme linked immunosorbent assays (ELISA). Biologicals. 18:1990;331.
4. Frey A., Di Canzio J., Zurakowski D. A statistically defined endpoint titer determination method for immunoassays. J. Immunol. Methods. 221:1998;35.
5. 2 und 3,3′,5,5′-Tetramethylbenzidin. J. Clin. Chem. Clin. Biochem. 23:1985;453.
6. Goka A.K.J., Farthing M.J.G. The use of 3,3′,5,5′-tetramethylbenzidine as a peroxidase substrate in microplate enzyme-linked immunosorbent assay. J. Immunoassay. 8:1987;29.
7. Harlow, E., Lane, D., 1988. Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 183 and 593.
8. Horn, J., Nebel-Schickel, H., 1991. Heterogeneous sandwich immunoassay using peroxidase label and reducing agent. Ger. Offen. DE 3930376.
9. Madersbacher S., Berger P. Double wavelength measurement of 3,3′,5,5′-tetramethylbenzidine (TMB) provides a three-fold enhancement of the ELISA measuring range. J. Immunol. Methods. 138:1991;121.
10. Natsuume-Sakai S., Motonishi K., Migita S. Quantitative estimations of five classes of immunoglobulin in inbred mouse strains. Immunology. 32:1977;861.
11. Porstmann T., Kiessig S.T. Enzyme immunoassay techniques. J. Immunol. Methods. 150:1992;5.
12. Quinn A., Harrison R., Jehanli A.M.T., Lunt G.G., Walsh S. An ELISA for the detection of anti-acetylcholine receptor antibodies using biotinylated α-bungarotoxin. J. Immunol. Methods. 107:1988;197.
13. Roberts I.M., Jones S.L., Premier R.R., Cox J.C. A comparison of the sensitivity and specificity of enzyme immunoassays and time-resolved fluoroimmunoassay. J. Immunol. Methods. 143:1991;49.
14. Rodbard, D., Hutt, D.M., 1974. Statistical analysis of radioimmunoassays and immunoradiometric (labelled antibody) assays: a generalized weighted, iterative, least-squares method for logistic curve fitting. In: Radioimmunoassay and Related Procedures in Medicine. International Atomic Energy Agency, Vienna, Austria, 1974, p. 165.
15. Voogd C.E., van der Stel J.J., Jacobs J.J.J.A.A. On the mutagenic action of some enzyme immunoassay substrates. J. Immunol. Methods. 36:1980;55.