An improved and reproducible protocol for the extraction of high quality fungal RNA from plant biomass substrates

Fungal Genetics and Biology

Volumn 72, Issue , 2014, Pages 201-206

  Patyshakuliyeva, Aleksandrina ^a,b   Mäkelä, Miia R ^c   Sietiö, Outi Maaria ^c   de Vries, Ronald P ^a,b   Hildén, Kristiina S ^c  

^a CBS KNAW FUNGAL BIODIVERSITY CENTRE   (Netherlands)

^b UTRECHT UNIVERSITY   (Netherlands)

^c UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Agaricus bisporus; CsCl gradient ultracentrifugation; Dichomitus squalens; Gene expression; Lignocellulose (plant biomass); RNA extraction

Indexed keywords

CESIUM CHLORIDE; FUNGAL RNA; MICROCRYSTALLINE CELLULOSE; RNA 18S; RNA 28S;

AGARICUS BISPORUS; ARTICLE; BIOMASS; CENTRIFUGATION; COMPOST; CONTROLLED STUDY; FUNGUS CULTURE; GENE EXPRESSION; NONHUMAN; NORWAY SPRUCE; QUALITY CONTROL; RELIABILITY; REPRODUCIBILITY; RNA EXTRACTION; RNA ISOLATION; WHEAT; DENSITY GRADIENT CENTRIFUGATION; GENE EXPRESSION PROFILING; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR BIOLOGY; PLANT; PROCEDURES;

BIOMASS; CENTRIFUGATION, DENSITY GRADIENT; GENE EXPRESSION PROFILING; MOLECULAR BIOLOGY; PLANTS; RNA, FUNGAL;

EID: 84908498234     PISSN: 10871845     EISSN: 10960937     Source Type: Journal    
DOI: 10.1016/j.fgb.2014.06.001     Document Type: Article

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