Gene expression monitoring in soils by mRNA analysis and gene lux fusions

Current Opinion in Biotechnology

Volumn 12, Issue 5, 2001, Pages 455-460

  Sayler, Gary S ^a   Fleming, James T ^a   Nivens, David E ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; MESSENGER RNA; PROTEIN LUXCDABE; UNCLASSIFIED DRUG;

BIOTECHNOLOGY; EXTRACTION; GENE EXPRESSION; GENE FUSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; REVIEW; SOIL MICROFLORA;

BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 0035476674     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(00)00245-7     Document Type: Review

References (43)

1. Environmental application of nucleic acid hybridization
2. Assessment of gene expression in the environment: Quantitative mRNA analysis in contaminated soils
3. Total diversity in soil and sediment communities - A review
4. Regulation of the synthesis of ribosomes and ribosomal components
5. Identification of the metabolically active member of a bacterial community in a polychlorinated biphenyl-polluted moorland soil
6. Microbial community analysis using environmental nucleic acid extracts
7. Direct recovery and molecular analysis of DNA and RNA from soil
8. Direct and simultaneous extraction of DNA and RNA from soil
9. Rapid method for direct extraction of mRNA from seeded soils
10. Isolation and characterization of RNA from low-biomass deep-subsurface sediments
11. The detection of Gram-negative bacterial mRNA from soil by RT-PCR
12. Quantitative relationship between naphthalene catabolic gene frequency and expression in predicting PAH degradation in soils at town gas manufacturing sites
13. Effect of 2-hydroxybenzoate on the rate of naphthalene mineralization in soil
14. Nucleic acid analytical approaches in bioremediation: Site assessment and characterization
15. Quantitation of fungal mRNAs in complex substrates by reverse transcription PCR and its application to Phanerochaete chrysosporium-colonized soil
16. Expression of lip genes during growth in soil and oxidation of anthracene by Phanerochaete chrysosporium
17. Manganese peroxidase mRNA and enzyme activity levels during bioremediation of polycyclic aromatic hydrocarbon-contaminated soil with Phanerochaete chrysosporium
18. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction
19. RNA fingerprinting and differential display using arbitrarily primed PCR
20. Optimization of differential display of prokaryotic mRNA: Application to pure culture and soil microcosms
21. Reduction of false positives in prokaryotic mRNA differential display
22. Screening for ribosomal-base false positives following prokaryotic differential display
23. Differential display of prokaryotic messenger RNA and application to soil microbial communities
24. Applications of reporter genes
25. Reporter gene bioassays in environmental analysis
26. Rapid, sensitive bioluminescent reporter technology for naphthalene exposure and biodegradation
27. A chromosomally based tod-luxCDABE whole-cell reporter for benzene, toluene, ethybenzene, and xylene (BTEX) sensing
28. Use of an ipb-lux fusion to study regulation of the isopropylbenzene catabolism operon of Pseudomonas putida RE204 and to detect hydrophobic pollutants in the environments
29. Construction of a bioluminescent reporter strain to detect polychlorinated biphenyls
30. A bioluminescent whole-cell reporter for detection of 2,4-dichlorophenoxyacetic acid and 2,4-dichlorophenol in soil
31. Whole cell- and protein-based biosensors for the detection of bioavailable heavy metals in environmental samples
32. A microbial biosensor to predict bioavailable nickel in soil and its transfer to plants
33. Molecular biology of bacterial bioluminescence
34. Induction of the tod operon by trichloroethylene in Pseudomonas putida TVA8
35. Use of bioluminescence markers to detect Pseudomonas spp. bacteria in the rhizosphere
36. Bioluminescent most-probable-number monitoring of a genetically engineered bacterium during a long-term contained field release
37. Simultaneous detection of gfp-marked Moraxella sp. G21r and lux-marked Ralstonia eutrophas H850Lr using most-probable-number method
38. Detection of a single genetically modified bacterial cell in soil by using charge coupled device-enhanced microscopy
39. Characterization of rhizosphere colonization by luminescent Enterobacter cloacae at population and single-cell levels
40. Controlled field release of a bioluminescent genetically engineered microorganism for bioremediation process monitoring and control
41. An intermediate-scale lysimeter facility for subsurface bioremediation
42. Physiological considerations of environmental applications of lux reporter fusions
43. Factors influencing expression of luxCDABE and nah genes in Pseudomonas putida RB1353(NAH7, pUTK9) in dynamic systems