The presence of humic substances and DNA in RNA extracts affects hybridization results

Applied and Environmental Microbiology

Volumn 66, Issue 10, 2000, Pages 4547-4554

  Alm, E W ^a   Zheng, D ^a   Raskin, L ^a  

^a CENTRAL MICHIGAN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; HUMIC SUBSTANCE; POLYADENYLIC POLYURIDYLIC ACID; RIBOSOME RNA; RNA;

ARTICLE; DILUTION; ESCHERICHIA COLI; EXTRACT; IMMUNOBLOTTING; MEMBRANE; NONHUMAN; OLIGONUCLEOTIDE PROBE; RNA HYBRIDIZATION;

DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HUMIC ACIDS; NUCLEIC ACID HYBRIDIZATION; REPRODUCIBILITY OF RESULTS; RNA, BACTERIAL; RNA, RIBOSOMAL; SENSITIVITY AND SPECIFICITY; SUPPORT, U.S. GOV'T, NON-P.H.S.;

EID: 0033775613     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.66.10.4547-4554.2000     Document Type: Article

References (30)

1. Appendix A
2. The oligonucleotide probe database
3. Critical factors influencing the recovery and integrity of rRNA extracted from environmental samples: Use of an optimized protocol to measure depth-related biomass distribution in freshwater sediments
4. Combination of 168 rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations
5. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology
6. Methanogenic population dynamics during start-up of anaerobic digesters treating municipal solid waste and biosolids
7. Polymerase chain reaction amplification of naphthalene-catabolic and 16S rRNA gene sequences from indigenous sediment bacteria
8. Isolation and purification of bacterial DNA from soils
9. Biogeochemical cycling in an organic-rich coastal marine basin-I. Methane sediment-water exchange processes
10. Direct extraction and purification of rRNA for ecological studies
11. Isolation and characterization of RNA from low-biomass deep-subsurface sediments
12. Critical evaluation of membrane supports for use in quantitative hybridizations
13. Molecular ecology of gastrointestinal ecosystems
14. Group-specific 168 rRNA hybridization probes to describe natural communities of methanogens
15. Characterization of microbial communities in anaerobic bioreactors using molecular probes
16. Community structure of a microbial mat: The phylogenetic dimension
17. DNA extraction for 168 rRNA gene analysis to determine genetic diversity in deep sediment communities
18. Protein content and protein synthesis rates of planktonic marine bacteria
19. Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology
20. Recovery of DNA from soils and sediments
21. High diversity in DNA of soil bacteria
22. Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction
23. Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction
24. Polyvinylpyrrolidone-agarose gel electrophoresis purification of polymerase chain reaction-amplifiable DNA from soils
25. Diversity of heterotrophic nitrogen fixation genes in a marine cyanobacterial mat
26. Characterization of universal small-subunit rRNA hybridization probes for quantitative molecular microbial ecology studies