Development of a fusion protein SNVP as substrate for assaying multi-serotype botulinum neurotoxins

Analytical Biochemistry

Volumn 463, Issue , 2014, Pages 75-81

  Luo, Sen ^a,b   Li, Tao ^a   Wang, Qin ^a   Tian, Renmao ^a   Liu, Hao ^a   Fang, Huali ^a   Chen, Fanghong ^a   Wang, Hui ^a  

^a BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

^b Wuxi Clinical College   (China)

Author keywords

Biological assay; Botulinum neurotoxin; SNAP 25; VAMP 2

Indexed keywords

AFFINITY CHROMATOGRAPHY; BIOACTIVITY; CLONING; COLUMN CHROMATOGRAPHY; ELECTROPHORESIS; ESCHERICHIA COLI; GENE ENCODING; PURIFICATION; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

BIOLOGICAL ASSAYS; BOTULINUM NEUROTOXINS; ESCHERICHIA COLI BL21; N-TERMINAL SEQUENCING; OPTIMAL CONDITIONS; SNAP-25; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; VAMP-2;

PROTEINS;

BOTULINUM TOXIN; HYBRID PROTEIN; PROTEINASE; SIGNAL PEPTIDE; SNVP FUSION PROTEIN; SYNAPTOBREVIN 2; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ESCHERICHIA COLI; GENE OVEREXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CLEAVAGE; PROTEIN PURIFICATION; TOXIN ANALYSIS; BIOASSAY; BIOSYNTHESIS; ENZYME SPECIFICITY; GENETICS; KINETICS; METABOLISM;

BIOLOGICAL ASSAY; BOTULINUM TOXINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; KINETICS; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY; SYNAPTOSOMAL-ASSOCIATED PROTEIN 25; VESICLE-ASSOCIATED MEMBRANE PROTEIN 2;

EID: 84908286627     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.06.019     Document Type: Article

References (41)

1. G. Schiavo, O. Rossetto, and C. Montecucco Clostridial neurotoxins as tools to investigate the molecular events of neurotransmitter release Semin. Cell Biol. 5 1994 221 229
2. G. Schiavo, A. Santucci, B.R. Dasgupta, P.P. Metha, J. Jontes, F. Benfenati, M.C. Wilson, and C. Montecucco Botulinum neurotoxin serotypes A and E cleave SNAP-25 at distinct COOH-terminal peptide bonds FEBS Lett. 335 1993 99 103
3. T. Binz, J. Blasi, S. Yamasaki, A. Baumeister, E. Link, T.C. Sudhof, R. Jahn, and H. Niemann Proteolysis of SNAP-25 by type E and A botulinum neurotoxins J. Biol. Chem. 269 1994 1617 1620
4. L.C. Williamson, J.L. Halpern, C. Montecucco, J.E. Brown, and E.A. Neale Clostridial neurotoxins and substrate proteolysis in intact neurons: botulinum neurotoxin C acts on synaptosomal-associated protein of 25 kDa J. Biol. Chem. 271 1996 7694 7699
5. C. Montecucco, and G. Schiavo Structure and function of tetanus and botulinum neurotoxins Q. Rev. Biophys. 28 1995 423 472
6. G. Schiavo, C. Malizio, W.S. Trimble, P. Polverino de Laureto, G. Milan, H. Sugiyama, E.A. Johnson, and C. Montecucco Botulinum G neurotoxin cleaves VAMP/synaptobrevin at a single Ala-Ala peptide bond J. Biol. Chem. 269 1994 20213 20216
7. D. Ungar, and F.M. Frederick SNARE protein structure and function Annu. Rev. Cell Dev. Biol. 19 2003 493 517
8. D.R. Ruge, F.M. Dunning, T.M. Piazza, B.E. Molles, M. Adler, F.N. Zeytin, and W.C. Tucker Detection of six serotypes of botulinum neurotoxin using fluorogenic reporters J. Anal. Biochem. 411 2011 200 209
9. D.A. Kautter, and H.M. Solomon Collaborative study of a method for the detection of Clostridium botulinum and its toxins in foods J. Assoc. Off. Anal. Chem. 60 1977 541 545
10. S. Cai, B.R. Singh, and S. Sharma Botulism diagnostics: from clinical symptoms to in vitro assays Crit. Rev. Microbiol. 33 2007 109 125
11. J.L. Ferreira, S.J. Eliasberg, P. Edmonds, and M.A. Harrison Comparison of the mouse bioassay and enzyme-linked immunosorbent assay procedures for the detection of type A botulinal toxin in food J. Food Prot. 67 2004 203 206
12. S.K. Sharma, J.L. Ferreira, B.S. Eblen, and R.C. Whiting Detection of type A, B, E, and F Clostridium botulinum neurotoxins in foods by using an amplified enzyme-linked immunosorbent assay with digoxigenin-labeled antibodies Appl. Environ. Microbiol. 72 2006 1231 1238
13. J. Ferreira, S. Maslanka, E. Johnson, and M. Goodnough Detection of botulinal neurotoxins A, B, E, and F by amplified enzyme-linked immunosorbent assay: collaborative study J. AOAC Int. 86 2003 314 331
14. B. Rowe, J. Schmidt, L. Smith, and S.A. Ahmed Rapid product analysis and increased sensitivity for quantitative determinations of botulinum neurotoxin proteolytic activity Anal. Biochem. 396 2010 188 193
15. K. Bagramyan, J. Barash, S. Arnon, and M. Kalkum Attomolar detection of botulinum toxin type A in complex biological matrices PLoS One 3 2008 e2041
16. K. Bagramyan, and M. Kalkum Ultrasensitive detection of botulinum neurotoxins and anthrax lethal factor in biological samples by ALISSA Methods Mol. Biol. 739 2011 23 36
17. M. Dong, W. Tepp, E. Johnson, and E. Chapman Using fluorescent sensors to detect botulinum neurotoxin activity in vitro and in living cells Proc. Natl. Acad. Sci. USA 101 2004 14701 14706
18. R. Rasooly, and P. Do Development of an in vitro activity assay as an alternative to the mouse bioassay for Clostridium botulinum neurotoxin type A Appl. Environ. Microbiol. 74 2008 4309 4313
19. J. Schmidt, and R. Stafford Fluorigenic substrates for the protease activities of botulinum neurotoxins, serotypes A, B, and F Appl. Environ. Microbiol. 69 2003 297 303
20. M. Gilmore, D. Williams, Y. Okawa, B. Holguin, N. James, J. Ross, K.R. Aoki, D. Jameson, and L. Steward Depolarization after resonance energy transfer (DARET): a sensitive fluorescence-based assay for botulinum neurotoxin protease activity Anal. Biochem. 413 2011 36 42
21. T.M. Piazza, D.S. Blehert, F.M. Dunning, B.M. Berlowski-Zier, F.N. Zeytin, M.D. Samuel, and W.C. Tucker In vitro detection and quantification of botulinum neurotoxin type E activity in avian blood Appl. Environ. Microbiol. 77 2011 7815 7822
22. J. Barr, H. Moura, A. Boyer, A. Woolfitt, S. Kalb, A. Pavlopoulos, L. McWilliams, J. Schmidt, R. Martinez, and D. Ashley Botulinum neurotoxin detection and differentiation by mass spectrometry: emerging infectious diseases Emerg. Infect. Dis. 11 2005 1578 1583
23. A. Boyer, M. Gallegos Candela, R. Lins, Z. Kuklenyik, A. Woolfitt, H. Moura, S. Kalb, C. Quinn, and J. Barr Quantitative mass spectrometry for bacterial protein toxins: a sensitive, specific, high-throughput tool for detection and diagnosis Molecules 16 2011 2391 2413
24. S. Kalb, C. Garcia Rodriguez, J. Lou, J. Baudys, T. Smith, J. Marks, L. Smith, J. Pirkle, and J. Barr Extraction of BoNT/A, /B, /E, and /F with a single, high affinity monoclonal antibody for detection of botulinum neurotoxin by Endopep-MS PLoS One 5 2010 e12237
25. D. Wang, J. Baudys, S. Kalb, and J. Barr Improved detection of botulinum neurotoxin type A in stool by mass spectrometry Anal. Biochem. 412 2011 67 73
26. D. Wang, J. Baudys, Y. Ye, J. Rees, J. Barr, J. Pirkle, and S. Kalb Improved detection of botulinum neurotoxin serotype A by Endopep-MS through peptide substrate modification Anal. Biochem. 432 2013 115 123
27. H. Poras, T. Ouimet, S.V. Orng, M.C. Fournié-Zaluski, M.R. Popoff, and B.P. Roques Detection and quantification of botulinum neurotoxin type A by a novel rapid in vitro fluorimetric assay Appl. Environ. Microbiol. 75 2009 4382 4390
28. S. Mangru, B.L. Bentz, T.J. Davis, N. Desai, P.J. Stabile, J.J. Schmidt, C.B. Millard, S. Bavari, and K. Kodukula Integrated bioassays in microfluidic devices: botulinum toxin assays J. Biomol. Screen. 10 2005 788 794
29. L. Li, and B.R. Singh High-level expression, purification, and characterization of recombinant type A botulinum neurotoxin light chain Protein Expr. Purif. 17 1999 339 344
30. J. Gilsdorf, N. Gul, and L.A. Smith Expression, purification, and characterization of Clostridium botulinum type B light chain Protein Expr. Purif. 46 2006 256 267
31. R. Agarwal, S. Eswaramoorthy, D. Kumaran, J.J. Dunn, and S. Swaminathan Cloning, high level expression, purification, and crystallization of the full length Clostridium botulinum neurotoxin type E light chain Protein Expr. Purif. 34 2004 95 102
32. J.W. Arndt, W. Yu, F. Bi, and R.C. Stevens Crystal structure of botulinum neurotoxin type G light chain: serotype divergence in substrate recognition Biochemistry 44 2005 9574 9580
33. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
34. T. Zor, and Z. Selinger Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies Anal. Biochem. 236 1996 302 308
35. S.A. Ahmed, and L.A. Smith Light chain of botulinum A neurotoxin expressed as an inclusion body from a synthetic gene is catalytically and functionally active J. Protein Chem. 19 2000 475 487
36. G. Schiavo, F. Benfenati, B. Poulain, O. Rossetto, P. Polverino de Laureto, B.R. DasGupta, and C. Montecucco Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin Nature 359 1992 832 835
37. C.C. Shone, and A.K. Roberts Peptide substrate specificity and properties of the zinc-endopeptidase activity of botulinum type B neurotoxin Eur. J. Biochem. 225 1994 263 270
38. J.J. Schmidt, and K.A. Bostian Proteolysis of synthetic peptides by type A botulinum neurotoxin J. Protein Chem. 14 1995 703 708
39. J.J. Schmidt, and R.G. Stafford Fluorogenic substrates for the protease activities of botulinum neurotoxins, serotypes A, B, and F Appl. Environ. Microbiol. 69 2003 297 303
40. H.B. Hines, A.D. Kim, R.G. Stafford, S.S. Badie, E.E. Brueggeman, D.J. Newman, and J.J. Schmidt Use of a recombinant fluorescent substrate with cleavage sites for all botulinum neurotoxins in high-throughput screening of natural product extracts for inhibitors of serotypes A, B, and E Appl. Environ. Microbiol. 73 2008 653 659
41. L.L. Simpson Identification of the major steps in botulinum toxin action Annu. Rev. Pharmacol. Toxicol. 44 2004 167 193