-
2
-
-
28544451327
-
Foundations for engineering biology
-
Endy D (2005) Foundations for engineering biology. Nature 438: 449-453.
-
(2005)
Nature
, vol.438
, pp. 449-453
-
-
Endy, D.1
-
3
-
-
84858408502
-
Building block synthesis using the polymerase chain assembly method
-
Marchand JA, Peccoud J (2012) Building block synthesis using the polymerase chain assembly method. Methods Mol Biol 852: 3-10.
-
(2012)
Methods Mol Biol
, vol.852
, pp. 3-10
-
-
Marchand, J.A.1
Peccoud, J.2
-
4
-
-
70449522250
-
Gene synthesis by integrated polymerase chain assembly and PCR amplification using a high-speed thermocycler
-
TerMaat JR, Pienaar E, Whitney SE, Mamedov TG, Subramanian A (2009) Gene synthesis by integrated polymerase chain assembly and PCR amplification using a high-speed thermocycler. J Microbiol Methods 79: 295-300.
-
(2009)
J Microbiol Methods
, vol.79
, pp. 295-300
-
-
Termaat, J.R.1
Pienaar, E.2
Whitney, S.E.3
Mamedov, T.G.4
Subramanian, A.5
-
5
-
-
34648837000
-
Rational de novo gene synthesis by rapid polymerase chain assembly (PCA) and expression of endothelial protein-C and thrombin receptor genes
-
Mamedov TG, Padhye NV, Viljoen H, Subramanian A (2007) Rational de novo gene synthesis by rapid polymerase chain assembly (PCA) and expression of endothelial protein-C and thrombin receptor genes. J Biotechnol 131: 379-387.
-
(2007)
J Biotechnol
, vol.131
, pp. 379-387
-
-
Mamedov, T.G.1
Padhye, N.V.2
Viljoen, H.3
Subramanian, A.4
-
6
-
-
0028074977
-
Assembly and cloning of coding sequences for neurotrophic factors directly from genomic DNA using polymerase chain reaction and uracil DNA glycosylase
-
Booth PM, Buchman GW, Rashtchian A (1994) Assembly and cloning of coding sequences for neurotrophic factors directly from genomic DNA using polymerase chain reaction and uracil DNA glycosylase. Gene 146: 303-308.
-
(1994)
Gene
, vol.146
, pp. 303-308
-
-
Booth, P.M.1
Buchman, G.W.2
Rashtchian, A.3
-
7
-
-
0028784138
-
Singlestep assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides
-
Stemmer WP, Crameri A, Ha KD, Brennan TM, Heyneker HL (1995) Singlestep assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides. Gene 164: 49-53.
-
(1995)
Gene
, vol.164
, pp. 49-53
-
-
Stemmer, W.P.1
Crameri, A.2
Ha, K.D.3
Brennan, T.M.4
Heyneker, H.L.5
-
8
-
-
45149118469
-
Engineering BioBrick vectors from BioBrick parts
-
Shetty RP, Endy D, Knight TF Jr. (2008) Engineering BioBrick vectors from BioBrick parts. J Biol Eng 2: 5.
-
(2008)
J Biol Eng
, vol.2
, pp. 5
-
-
Shetty, R.P.1
Endy, D.2
Knight, T.F.3
-
9
-
-
80053175916
-
BglBrick vectors and datasheets: A synthetic biology platform for gene expression
-
Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, et al. (2011) BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng 5: 12.
-
(2011)
J Biol Eng
, vol.5
, pp. 12
-
-
Lee, T.S.1
Krupa, R.A.2
Zhang, F.3
Hajimorad, M.4
Holtz, W.J.5
-
10
-
-
84903366227
-
BioBrick assembly standards and techniques and associated software tools
-
Rokke G, Korvald E, Pahr J, Oyas O, Lale R (2014) BioBrick assembly standards and techniques and associated software tools. Methods Mol Biol 1116: 1-24.
-
(2014)
Methods Mol Biol
, vol.1116
, pp. 1-24
-
-
Rokke, G.1
Korvald, E.2
Pahr, J.3
Oyas, O.4
Lale, R.5
-
11
-
-
67349270900
-
Enzymatic assembly of DNA molecules up to several hundred kilobases
-
Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, 3rd, et al. (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 6: 343-345.
-
(2009)
Nat Methods
, vol.6
, pp. 343-345
-
-
Gibson, D.G.1
Young, L.2
Chuang, R.Y.3
Venter, J.C.4
Hutchison, C.A.5
-
12
-
-
78049323797
-
Chemical synthesis of the mouse mitochondrial genome
-
Gibson DG, Smith HO, Hutchison CA 3rd, Venter JC, Merryman C (2010) Chemical synthesis of the mouse mitochondrial genome. Nat Methods 7: 901-903.
-
(2010)
Nat Methods
, vol.7
, pp. 901-903
-
-
Gibson, D.G.1
Smith, H.O.2
Hutchison, C.A.3
Venter, J.C.4
Merryman, C.5
-
13
-
-
77953584054
-
Creation of a bacterial cell controlled by a chemically synthesized genome
-
Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY, et al. (2010) Creation of a bacterial cell controlled by a chemically synthesized genome. Science 329: 52-56.
-
(2010)
Science
, vol.329
, pp. 52-56
-
-
Gibson, D.G.1
Glass, J.I.2
Lartigue, C.3
Noskov, V.N.4
Chuang, R.Y.5
-
14
-
-
84877274681
-
The MASTER (methylationassisted tailorable ends rational) ligation method for seamless DNA assembly
-
Chen WH, Qin ZJ, Wang J, Zhao GP (2013) The MASTER (methylationassisted tailorable ends rational) ligation method for seamless DNA assembly. Nucleic Acids Res 41: e93.
-
(2013)
Nucleic Acids Res
, vol.41
, pp. e93
-
-
Chen, W.H.1
Qin, Z.J.2
Wang, J.3
Zhao, G.P.4
-
15
-
-
56649114274
-
A one pot, one step, precision cloning method with high throughput capability
-
Engler C, Kandzia R, Marillonnet S (2008) A one pot, one step, precision cloning method with high throughput capability. PLoS One 3: e3647.
-
(2008)
PLoS One
, vol.3
-
-
Engler, C.1
Kandzia, R.2
Marillonnet, S.3
-
16
-
-
79958279010
-
Generation of families of construct variants using golden gate shuffling
-
Engler C, Marillonnet S (2011) Generation of families of construct variants using golden gate shuffling. Methods Mol Biol 729: 167-181.
-
(2011)
Methods Mol Biol
, vol.729
, pp. 167-181
-
-
Engler, C.1
Marillonnet, S.2
-
17
-
-
65849183178
-
Golden gate shuffling: A one-pot DNA shuffling method based on type IIs restriction enzymes
-
Engler C, Gruetzner R, Kandzia R, Marillonnet S (2009) Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes. PLoS One 4: e5553.
-
(2009)
PLoS One
, vol.4
-
-
Engler, C.1
Gruetzner, R.2
Kandzia, R.3
Marillonnet, S.4
-
18
-
-
73449145712
-
Silent (synonymous) SNPs: Should we care about them?
-
Hunt R, Sauna ZE, Ambudkar SV, Gottesman MM, Kimchi-Sarfaty C (2009) Silent (synonymous) SNPs: should we care about them? Methods Mol Biol 578: 23-39.
-
(2009)
Methods Mol Biol
, vol.578
, pp. 23-39
-
-
Hunt, R.1
Sauna, Z.E.2
Ambudkar, S.V.3
Gottesman, M.M.4
Kimchi-Sarfaty, C.5
-
19
-
-
34548726841
-
Silent SNPs: Impact on gene function and phenotype
-
Komar AA (2007) Silent SNPs: impact on gene function and phenotype. Pharmacogenomics 8: 1075-1080.
-
(2007)
Pharmacogenomics
, vol.8
, pp. 1075-1080
-
-
Komar, A.A.1
-
20
-
-
33846588874
-
Genetics. SNPs, silent but not invisible
-
Komar AA (2007) Genetics. SNPs, silent but not invisible. Science 315: 466-467.
-
(2007)
Science
, vol.315
, pp. 466-467
-
-
Komar, A.A.1
-
21
-
-
77952477753
-
Pairwise selection assembly for sequence-independent construction of long-length DNA
-
Blake WJ, Chapman BA, Zindal A, Lee ME, Lippow SM, et al. (2010) Pairwise selection assembly for sequence-independent construction of long-length DNA. Nucleic Acids Res 38: 2594-2602.
-
(2010)
Nucleic Acids Res
, vol.38
, pp. 2594-2602
-
-
Blake, W.J.1
Chapman, B.A.2
Zindal, A.3
Lee, M.E.4
Lippow, S.M.5
-
22
-
-
84887606266
-
One-step assembly and targeted integration of multigene constructs assisted by the I-SceI meganuclease in Saccharomyces cerevisiae
-
Kuijpers NG, Chroumpi S, Vos T, Solis-Escalante D, Bosman L, et al. (2013) One-step assembly and targeted integration of multigene constructs assisted by the I-SceI meganuclease in Saccharomyces cerevisiae. FEMS Yeast Res 13: 769-781.
-
(2013)
FEMS Yeast Res
, vol.13
, pp. 769-781
-
-
Kuijpers, N.G.1
Chroumpi, S.2
Vos, T.3
Solis-Escalante, D.4
Bosman, L.5
-
23
-
-
0027513318
-
Meganuclease I-SceI as a tool for the easy subcloning of large DNA fragments devoid of selection marker
-
Viret JF (1993) Meganuclease I-SceI as a tool for the easy subcloning of large DNA fragments devoid of selection marker. Biotechniques 14: 325-326.
-
(1993)
Biotechniques
, vol.14
, pp. 325-326
-
-
Viret, J.F.1
-
24
-
-
0026573892
-
Site-specific recombination determined by I-SceI, a mitochondrial group i intron-encoded endonuclease expressed in the yeast nucleus
-
Plessis A, Perrin A, Haber JE, Dujon B (1992) Site-specific recombination determined by I-SceI, a mitochondrial group I intron-encoded endonuclease expressed in the yeast nucleus. Genetics 130: 451-460.
-
(1992)
Genetics
, vol.130
, pp. 451-460
-
-
Plessis, A.1
Perrin, A.2
Haber, J.E.3
Dujon, B.4
-
25
-
-
0027753790
-
In vitro protein splicing of purified precursor and the identification of a branched intermediate
-
Xu MQ, Southworth MW, Mersha FB, Hornstra LJ, Perler FB (1993) In vitro protein splicing of purified precursor and the identification of a branched intermediate. Cell 75: 1371-1377.
-
(1993)
Cell
, vol.75
, pp. 1371-1377
-
-
Xu, M.Q.1
Southworth, M.W.2
Mersha, F.B.3
Hornstra, L.J.4
Perler, F.B.5
-
26
-
-
80054973992
-
Development of a gene cloning system in a fast-growing and moderately thermophilic Streptomyces species and heterologous expression of Streptomyces antibiotic biosynthetic gene clusters
-
Chen W, Qin Z (2011) Development of a gene cloning system in a fast-growing and moderately thermophilic Streptomyces species and heterologous expression of Streptomyces antibiotic biosynthetic gene clusters. BMC Microbiol 11: 243.
-
(2011)
BMC Microbiol
, vol.11
, pp. 243
-
-
Chen, W.1
Qin, Z.2
-
27
-
-
84863090270
-
Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology
-
Naatsaari L, Mistlberger B, Ruth C, Hajek T, Hartner FS, et al. (2012) Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology. PLoS One 7: e39720.
-
(2012)
PLoS One
, vol.7
-
-
Naatsaari, L.1
Mistlberger, B.2
Ruth, C.3
Hajek, T.4
Hartner, F.S.5
-
28
-
-
0037603297
-
Targeted modification of a human beta-globin locus BAC clone using GET Recombination and an I-Scei counterselection cassette
-
Jamsai D, Orford M, Nefedov M, Fucharoen S, Williamson R, et al. (2003) Targeted modification of a human beta-globin locus BAC clone using GET Recombination and an I-Scei counterselection cassette. Genomics 82: 68-77.
-
(2003)
Genomics
, vol.82
, pp. 68-77
-
-
Jamsai, D.1
Orford, M.2
Nefedov, M.3
Fucharoen, S.4
Williamson, R.5
-
29
-
-
0003869903
-
-
Norwich Research Park, Norwich NR4 7UH, England: The John Innes Foundation Press
-
Kieser, Buttner MJ, Chater KF, Hopwood DA (2000) Practical Streptomyces Genetics. Norwich Research Park, Norwich NR4 7UH, England: The John Innes Foundation Press.
-
(2000)
Practical Streptomyces Genetics
-
-
Kieser Buttner, M.J.1
Chater, K.F.2
Hopwood, D.A.3
-
30
-
-
0021943518
-
Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors
-
Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119.
-
(1985)
Gene
, vol.33
, pp. 103-119
-
-
Yanisch-Perron, C.1
Vieira, J.2
Messing, J.3
-
31
-
-
0036739377
-
Conditionally amplifiable BACs: Switching from single-copy to high-copy vectors and genomic clones
-
Wild J, Hradecna Z, Szybalski W (2002) Conditionally amplifiable BACs: switching from single-copy to high-copy vectors and genomic clones. Genome Res 12: 1434-1444.
-
(2002)
Genome Res
, vol.12
, pp. 1434-1444
-
-
Wild, J.1
Hradecna, Z.2
Szybalski, W.3
-
32
-
-
3042791271
-
Lambda red-mediated genetic manipulation of antibiotic-producing Streptomyces
-
Gust B, Chandra G, Jakimowicz D, Yuqing T, Bruton CJ, et al. (2004) Lambda red-mediated genetic manipulation of antibiotic-producing Streptomyces. Adv Appl Microbiol 54: 107-128.
-
(2004)
Adv Appl Microbiol
, vol.54
, pp. 107-128
-
-
Gust, B.1
Chandra, G.2
Jakimowicz, D.3
Yuqing, T.4
Bruton, C.J.5
-
33
-
-
7444229263
-
Many paths to many clones: A comparative look at high-throughput cloning methods
-
Marsischky G, LaBaer J (2004) Many paths to many clones: a comparative look at high-throughput cloning methods. Genome Res 14: 2020-2028.
-
(2004)
Genome Res
, vol.14
, pp. 2020-2028
-
-
Marsischky, G.1
Labaer, J.2
-
34
-
-
0026645203
-
Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp
-
Bierman M, Logan R, O'Brien K, Seno ET, Rao RN, et al. (1992) Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116: 43-49.
-
(1992)
Gene
, vol.116
, pp. 43-49
-
-
Bierman, M.1
Logan, R.2
O'Brien, K.3
Seno, E.T.4
Rao, R.N.5
-
36
-
-
0025357616
-
Purification and characterization of the in vitro activity of I-Sce I, a novel and highly specific endonuclease encoded by a group i intron
-
Monteilhet C, Perrin A, Thierry A, Colleaux L, Dujon B (1990) Purification and characterization of the in vitro activity of I-Sce I, a novel and highly specific endonuclease encoded by a group I intron. Nucleic Acids Res 18: 1407-1413.
-
(1990)
Nucleic Acids Res
, vol.18
, pp. 1407-1413
-
-
Monteilhet, C.1
Perrin, A.2
Thierry, A.3
Colleaux, L.4
Dujon, B.5
-
37
-
-
33748800981
-
TAR cloning: Insights into gene function, longrange haplotypes and genome structure and evolution
-
Kouprina N, Larionov V (2006) TAR cloning: insights into gene function, longrange haplotypes and genome structure and evolution. Nat Rev Genet 7: 805-812.
-
(2006)
Nat Rev Genet
, vol.7
, pp. 805-812
-
-
Kouprina, N.1
Larionov, V.2
-
38
-
-
84860778298
-
Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting
-
Fu J, Bian X, Hu S, Wang H, Huang F, et al. (2012) Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting. Nat Biotechnol 30: 440-446.
-
(2012)
Nat Biotechnol
, vol.30
, pp. 440-446
-
-
Fu, J.1
Bian, X.2
Hu, S.3
Wang, H.4
Huang, F.5
-
39
-
-
84859739756
-
Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination
-
Zhang L, Zhao G, Ding X (2011) Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination. Sci Rep 1: 141.
-
(2011)
Sci Rep
, vol.1
, pp. 141
-
-
Zhang, L.1
Zhao, G.2
Ding, X.3
-
40
-
-
79951486698
-
DNA cleavage is independent of synapsis during Streptomyces phage phiBT1 integrase-mediated site-specific recombination
-
Zhang L, Wang L, Wang J, Ou X, Zhao G, et al. (2010) DNA cleavage is independent of synapsis during Streptomyces phage phiBT1 integrase-mediated site-specific recombination. J Mol Cell Biol 2: 264-275.
-
(2010)
J Mol Cell Biol
, vol.2
, pp. 264-275
-
-
Zhang, L.1
Wang, L.2
Wang, J.3
Ou, X.4
Zhao, G.5
-
41
-
-
66249145050
-
Creation of a type IIS restriction endonuclease with a long recognition sequence
-
Lippow SM, Aha PM, Parker MH, Blake WJ, Baynes BM, et al. (2009) Creation of a type IIS restriction endonuclease with a long recognition sequence. Nucleic Acids Res 37: 3061-3073.
-
(2009)
Nucleic Acids Res
, vol.37
, pp. 3061-3073
-
-
Lippow, S.M.1
Aha, P.M.2
Parker, M.H.3
Blake, W.J.4
Baynes, B.M.5
-
42
-
-
0023715368
-
Improved broad-hostrange plasmids for DNA cloning in gram-negative bacteria
-
Keen NT, Tamaki S, Kobayashi D, Trollinger D (1988) Improved broad-hostrange plasmids for DNA cloning in gram-negative bacteria. Gene 70: 191-197.
-
(1988)
Gene
, vol.70
, pp. 191-197
-
-
Keen, N.T.1
Tamaki, S.2
Kobayashi, D.3
Trollinger, D.4
-
43
-
-
0034612342
-
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
-
Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97: 6640-6645.
-
(2000)
Proc Natl Acad Sci U S A
, vol.97
, pp. 6640-6645
-
-
Datsenko, K.A.1
Wanner, B.L.2
-
44
-
-
55749088258
-
Nitrogen control in Mycobacterium smegmatis: Nitrogen-dependent expression of ammonium transport and assimilation proteins depends on the OmpR-type regulator GlnR
-
Amon J, Brau T, Grimrath A, Hanssler E, Hasselt K, et al. (2008) Nitrogen control in Mycobacterium smegmatis: nitrogen-dependent expression of ammonium transport and assimilation proteins depends on the OmpR-type regulator GlnR. J Bacteriol 190: 7108-7116.
-
(2008)
J Bacteriol
, vol.190
, pp. 7108-7116
-
-
Amon, J.1
Brau, T.2
Grimrath, A.3
Hanssler, E.4
Hasselt, K.5
-
45
-
-
0037335488
-
Bacteriophage T4 genome
-
Miller ES, Kutter E, Mosig G, Arisaka F, Kunisawa T, et al. (2003) Bacteriophage T4 genome. Microbiol Mol Biol Rev 67: 86-156.
-
(2003)
Microbiol Mol Biol Rev
, vol.67
, pp. 86-156
-
-
Miller, E.S.1
Kutter, E.2
Mosig, G.3
Arisaka, F.4
Kunisawa, T.5
|