Building block synthesis using the polymerase chain assembly method

Methods in Molecular Biology

Volumn 852, Issue , 2012, Pages 3-10

  Marchand, Julie A ^a   Peccoud, Jean ^a  

^a VIRGINIA BIOINFORMATICS INSTITUTE   (United States)

Author keywords

Building blocks; Computer assisted design; DNA fabrication; Gene synthesis; Polymerase chain assembly

Indexed keywords

DNA; OLIGODEOXYRIBONUCLEOTIDE;

ARTICLE; BIOSYNTHESIS; CHROMOSOME; COMPUTER AIDED DESIGN; COMPUTER PROGRAM; DNA SEQUENCE; ESCHERICHIA COLI; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

CHROMOSOMES, FUNGAL; CLONING, MOLECULAR; COMPUTER-AIDED DESIGN; DNA; ESCHERICHIA COLI; OLIGODEOXYRIBONUCLEOTIDES; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS, DNA; SOFTWARE;

EID: 84858408502     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-564-0_1     Document Type: Article

References (5)

1. Czar MJ, Anderson JC, Bader JS and Peccoud J. (2009) Gene synthesis demystifi ed. Trends Biotechnol 27:63-72.
2. Xiong AS, Peng RH, Zhuang J, Gao F, Li Y, Cheng Z M and Yao, QH (2008) Chemical gene synthesis: Strategies, softwares, error corrections, and applications. FEMS Microbiol 32:522-540.
3. Cello J, Paul AV and Wimmer E (2002) Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template. Science 297:1016-1018.
4. Dymond J, Scheifele L, Richardson S, Lee P, Chandrasegaran S, Bader J and Boeke JD (2009) Teaching Synthetic Biology, Bioinformatics, and Engineering to Undergraduates: The Interdisciplinary Build-a-Genome Course. Genetics 18:13-21.
5. Richardson SM, Wheelan SJ, Yarrington, RM and Boeke, JD (2006) GeneDesign: rapid, automated design of multikilobase synthetic genes. Genome Res 16:550-6.