Rational de novo gene synthesis by rapid polymerase chain assembly (PCA) and expression of endothelial protein-C and thrombin receptor genes

Journal of Biotechnology

Volumn 131, Issue 4, 2007, Pages 379-387

  Mamedov, Tarlan G ^a   Padhye, Nisha V ^a,b   Viljoen, Hendrik ^a   Subramanian, Anuradha ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

^b Megabase Research Products   (United States)

Author keywords

Error comparison; Gene synthesis and expression; High speed polymerase chain assembly; PCRJet thermocycler; Thermal damage of DNA and control

Indexed keywords

ERROR COMPARISON; GENE SYNTHESIS; HIGH-SPEED POLYMERASE CHAIN ASSEMBLY; POLYMERASE CHAIN ASSEMBLY (PCA); THERMAL DAMAGE;

DNA; ENZYME KINETICS; PROTEINS; SYNTHESIS (CHEMICAL); THERMAL EFFECTS;

GENE EXPRESSION;

OLIGONUCLEOTIDE; PROTEIN C; PROTEIN C RECEPTOR; RECEPTOR SUBTYPE; THROMBIN RECEPTOR; THROMBOMODULIN; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; CONTROL STRATEGY; DNA SEQUENCE; ENDOTHELIUM CELL; ERROR; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE CONSTRUCT; GENE EXPRESSION; GENE SYNTHESIS; GLYCOSYLATION; MOLECULAR CLONING; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECEPTOR GENE; TEMPERATURE;

BLOOD COAGULATION FACTORS; GENE EXPRESSION REGULATION; HUMANS; MUTATION; PICHIA; POLYMERASE CHAIN REACTION; RECEPTORS, CELL SURFACE; RECEPTORS, THROMBIN; RECOMBINANT PROTEINS; TEMPERATURE; TIME FACTORS;

PICHIA PASTORIS;

EID: 34648837000     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2007.08.010     Document Type: Article

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