Discovery of a reductase-producing strain recombinant E. coli CCZU-A13 using colorimetric screening and its whole cell-catalyzed biosynthesis of ethyl (R)-4-chloro-3-hydroxybutanoate

Bioresource Technology

Volumn 172, Issue , 2014, Pages 342-348

  He, Yu Cai ^a   Zhang, Dan Ping ^a   Tao, Zhi Cheng ^a   Zhang, Xian ^b   Yang, Zhen Xing ^a  

^a CHANGZHOU UNIVERSITY   (China)

^b Rice University   (United States)

Author keywords

Asymmetric bioreduction; Biphasic system; Ethyl (R) 4 chloro 3 hydroxybutanoate; Ethyl 4 chloro 3 oxobutanoate; Recombinant E. coli CCZU A13

Indexed keywords

BIOCHEMISTRY; CARBONYL COMPOUNDS; COLORIMETRY; DATA MINING; STEREOSELECTIVITY;

ASYMMETRIC BIOREDUCTION; BIPHASIC SYSTEMS; ETHYL (R)-4-CHLORO-3-HYDROXYBUTANOATE; ETHYL 4-CHLORO-3-OXOBUTANOATE; RECOMBINANT E. COLI;

ESCHERICHIA COLI;

ACETIC ACID; BETA CARBONYL CARBOXYLIC ESTER REDUCTASE; BUTYRIC ACID; ETHYL CHLORO 3 HYDROXYBUTANOATE; N BUTYL ACETATE; OXIDOREDUCTASE; UNCLASSIFIED DRUG; WATER; ACETOACETIC ACID DERIVATIVE; ETHYL 4-CHLORO-3-OXOBUTANOATE; NICOTINAMIDE ADENINE DINUCLEOTIDE; RECOMBINANT PROTEIN;

BIOLOGICAL PRODUCTION; BIOTECHNOLOGY; BIOTRANSFORMATION; COLIFORM BACTERIUM; COLORIMETRY; ENZYME ACTIVITY; GENE EXPRESSION; GENOME; INDUSTRIAL PRODUCTION; REDUCTION;

ARTICLE; BACTERIAL STRAIN; BIOSYNTHESIS; BIOTRANSFORMATION; COLORIMETRY; ESCHERICHIA COLI; HIGH THROUGHPUT SCREENING; NONHUMAN; CATALYSIS; CLASSIFICATION; GENETIC ENHANCEMENT; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PHYSIOLOGY; PROCEDURES; SPECIES DIFFERENCE;

ACETOACETATES; CATALYSIS; COLORIMETRY; ESCHERICHIA COLI; GENETIC ENHANCEMENT; NAD; RECOMBINANT PROTEINS; SPECIES SPECIFICITY;

EID: 84907755763     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2014.09.062     Document Type: Article

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