Purification and characterization of a novel NADH-dependent carbonyl reductase from Pichia stipitis involved in biosynthesis of optically pure ethyl (S)-4-chloro-3-hydroxybutanoate

Bioresource Technology

Volumn 102, Issue 2, 2011, Pages 1733-1739

  Cao, Hou ^a   Mi, Lan ^a   Ye, Qi ^a,b,c   Zang, Guanglou ^c   Yan, Ming ^a   Wang, Yan ^a   Zhang, Yueyuan ^b   Li, Ximu ^a   Xu, Lin ^a   Xiong, Jian ^b   Ouyang, Pingkai ^a,b   Ying, Hanjie ^a,b  

^a NANJING TECH UNIVERSITY   (China)

^b National Engineering Technique Research Center for Biotechnology   (China)

^c National Center for Quality Supervision and Test of Chinese Liquor   (China)

Author keywords

Carbonyl reductase; NADH dependent; Pichia stipitis; Short chain dehydrogenases reductases; Substrate specificity

Indexed keywords

CARBONYL REDUCTASE; NADH-DEPENDENT; PICHIA STIPITIS; SHORT-CHAIN DEHYDROGENASES/REDUCTASES; SUBSTRATE SPECIFICITY;

AMMONIUM COMPOUNDS; BIOCHEMISTRY; CARBONYLATION; ENANTIOSELECTIVITY; ENZYMES; ESTERS; GEL PERMEATION CHROMATOGRAPHY; PURIFICATION; SUBSTRATES; YEAST;

ENZYME ACTIVITY;

AMMONIUM SULFATE; ETHYL 4 CHLORO 3 HYDROXYBUTANOATE; ETHYL 4 CHLORO 3 OXOBUTANOATE; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEPENDENT CARBONYL REDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

BIOTECHNOLOGY; CHROMATOGRAPHY; ENZYME ACTIVITY; ESTER; PRECIPITATION (CHEMISTRY); SUBSTRATE; YEAST;

ARTICLE; CATALYSIS; CHROMATOGRAPHY; CONTROLLED STUDY; ELECTRON; ENANTIOMER; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME STABILITY; HUMAN; NONHUMAN; PICHIA STIPITIS; PRECIPITATION; PRIORITY JOURNAL; SYNTHESIS;

ACETOACETATES; ALCOHOL OXIDOREDUCTASES; AMINO ACID SEQUENCE; CHELATING AGENTS; COENZYMES; DETERGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME INHIBITORS; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; NAD; OPTICAL PHENOMENA; PICHIA; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; TEMPERATURE;

PICHIA STIPITIS;

EID: 78650681555     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2010.08.072     Document Type: Article

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