Engineering an enhanced, thermostable, monomeric bacterial luciferase gene as a reporter in plant protoplasts

PLoS ONE

Volumn 9, Issue 10, 2014, Pages

  Cui, Boyu ^a   Zhang, Lifeng ^a   Song, Yunhong ^a   Wei, Jinsong ^a   Li, Changfu ^a   Wang, Tietao ^a   Wang, Yao ^a   Zhao, Tianyong ^a   Shen, Xihui ^a  

^a NORTHWEST A F UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

LUCIFERASE; MONOMER; ALKANAL MONOOXYGENASE (FMN LINKED); CODON; HYBRID PROTEIN;

AMINO ACID SUBSTITUTION; ARABIDOPSIS THALIANA; ARTICLE; BACTERIAL CELL; BACTERIAL GENE; CHEMICAL MUTAGENESIS; CODON USAGE; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; ELUXAB GENE; ENZYME ACTIVITY; ESCHERICHIA COLI; EXPRESSION VECTOR; FUNGAL CELL; GENE EXPRESSION; GENE FUSION; GENE MUTATION; GENETIC ENGINEERING; MAIZE; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; NUCLEOTIDE SEQUENCE; PHOTORHABDUS LUMINESCENS; PLANT CELL; POLYMERASE CHAIN REACTION; PROTOPLAST; QUANTUM YIELD; REPORTER GENE; SACCHAROMYCES CEREVISIAE; THERMOSTABILITY; YERSINIA PSEUDOTUBERCULOSIS; CHEMICAL STRUCTURE; CHEMISTRY; CODON; ENZYME STABILITY; GENE ORDER; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; MUTATION; PH; PLANT; PLANT GENE; PLASMID; PROTEIN CONFORMATION; TEMPERATURE;

BACTERIA (MICROORGANISMS);

CODON; DNA SHUFFLING; ENZYME STABILITY; GENE EXPRESSION; GENE ORDER; GENES, PLANT; GENES, REPORTER; HYDROGEN-ION CONCENTRATION; LUCIFERASES, BACTERIAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PLANTS; PLASMIDS; PROTEIN CONFORMATION; PROTOPLASTS; RECOMBINANT FUSION PROTEINS; TEMPERATURE; ZEA MAYS;

EID: 84907546569     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0107885     Document Type: Article

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