Random mutagenesis of bacterial luciferase: Critical role of Glu 175 in the control of luminescence decay

Biochemical Journal

Volumn 385, Issue 2, 2005, Pages 575-580

  Hosseinkhani, Saman ^a,b   Szittner, Rose ^b   Meighen, Edward A ^b  

^a TARBIAT MODARES UNIVERSITY   (Iran)

^b MCGILL UNIVERSITY   (Canada)

Author keywords

Bacterial luciferase; Bioluminescence; Decay rate; Error prone PCR; Fluorescence quenching; Random mutagenesis

Indexed keywords

ALDEHYDES; DECOMPOSITION; ENZYMES; LUMINESCENCE;

DECAY RATE; LUCIFERASE; MUTATION; PHOTOBACTERIUM;

BACTERIA;

ALDEHYDE; BACTERIAL ENZYME; FLAVINE MONONUCLEOTIDE; GLUTAMIC ACID; GLYCINE; LUCIFERASE; PROTEIN LUXAB; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BIOLUMINESCENCE; CONTROLLED STUDY; DECOMPOSITION; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SUBUNIT; FLUORESCENCE; MUTAGENESIS; NONHUMAN; PHOTOBACTERIUM LEIOGNATHI; PHOTOBACTERIUM PHOSPHOREUM; PHOTORHABDUS LUMINESCENS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTUM YIELD; VIBRIO FISCHERI; VIBRIO HARVEYI;

ALDEHYDES; BACTERIAL PROTEINS; CLONING, MOLECULAR; FLAVIN MONONUCLEOTIDE; FLAVINS; GLUTAMIC ACID; LUCIFERASES, BACTERIAL; LUMINESCENCE; LUMINESCENT MEASUREMENTS; LUMINESCENT PROTEINS; MODELS, CHEMICAL; MUTAGENESIS; PHOTOBACTERIUM; PHOTORHABDUS; RESEARCH DESIGN; SUBSTRATE SPECIFICITY; VIBRIO;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PHOTOBACTERIUM LEIOGNATHI; PHOTOBACTERIUM PHOSPHOREUM; PHOTORHABDUS LUMINESCENS; VIBRIO FISCHERI; VIBRIO HARVEYI; XENORHABDUS;

EID: 12844268096     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20040863     Document Type: Article

References (30)

1. Hastings, J. W. and Gibson, Q. H. (1963) Intermediates in the bioluminescent oxidation of reduced flavin mononucleotide. J. Biol. Chem. 238, 2537-2554
2. Gibson, Q. H. and Hastings, J. W. (1962) The oxidation of reduced flavin mononucleotide by molecular oxygen. Biochem. J. 83, 368-377
3. Fisher, A. J., Thompson, T. B., Thoden, J. B., Baldwin, T. O. and Rayment, I. (1996) The 1.5 Å resolution crystal structure of bacterial luciferase in low salt conditions. J. Biol. Chem. 271, 21956-21968
4. AbouKhair, N. K., Ziegler, M. M. and Baldwin, T. O. (1985) Bacterial luciferase: demonstration of a catalytically competent altered conformational state following a single turnover. Biochemistry 24, 3942-3947
5. Nealson, K. H. (1978) Isolation, identification and manipulation of luminous bacteria. Methods Enzymol. 57, 153-166
6. Nealson, K. H. and Hastings, J. W. (1979) Bacterial bioluminescence: its control and ecological significance. Microbiol. Rev. 43, 496-518
7. Szittner, R. and Meighen, E. (1990) Nucleotide sequence, expression, and properties of luciferase coded by lux genes from a terrestrial bacterium. J. Biol. Chem. 265, 16581-16587
8. Valkova, N., Szittner, R. and Meighen, E. A. (1999) Control of luminescence decay and flavin binding by the LuxA carboxyl-terminal regions in chimeric bacterial luciferases. Biochemistry 38, 13820-13828
9. Meighen, E. A. and Hastings, J. W. (1971) Binding site determination from kinetic data. Reduced flavin mononucleotide binding to bacterial luciferase. J. Biol. Chem. 246, 7666-7674
10. Cline, T. W. and Hastings, J. W. (1974) Mutated luciferases with altered bioluminescence emission spectra. J. Biol. Chem. 249, 4668-4669
11. Spee, J. H., Vos, W. M. D. and Kuipers, O. P. (1993) Efficient random mutagenesis method with adjustable mutation frequency by use of PCR and dITP. Nucleic Acids Res. 21, 777-778
12. Leung, D. W., Chen, E. and Geoddel, D. V. (1989) A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction. Technique 1, 11-15
13. Gunsalus-Miguel, A., Meighen, E. A., Nicoli, M. Z., Nealson, K. H. and Hastings, J. W. (1972) Purification and properties of bacterial luciferases. J. Biol. Chem. 247, 398-404
14. Bradford, M. M. (1976) A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
15. Meighen, E. A. and Mackenzie, R. E. (1973) Flavin specificity of enzyme-substrate intermediates in the bacterial bioluminescent reaction. Biochemistry 12, 1482-1491
16. Hosseinkhani, S., Szittner, R., Nemat-Gorgani, M. and Meighen, E. (2003) Adsorptive immobilization of bacterial luciferase on alkyl-substituted Sepharose 4B. Enzyme Microb. Technol. 32, 186-193
17. Mitchell, G. W. and Hastings, J. W. (1971) Stable, inexpensive, solid-state photo multiplier photometer. Anal. Biochem. 39, 243-250
18. Li, Z., Szittner, R. and Meighen, E. A. (1993) Subunit interactions and the role of the luxA polypeptide in controlling thermal stability and catalytic properties in recombinant luciferase hybrids. Biochim. Biophys. Acta 1158, 137-145
19. Lin, L. Y., Sulea, T., Szittner, R., Vassilyev, V., Purisima, E. O. and Meighen, A. (2001) Modeling of bacterial luciferase-flavin mononucleotide complex combining flexible docking with structure-activity data. Protein Sci. 10, 1563-1571
20. Chen, L. H. and Baldwin, T. O. (1989) Random and site-directed mutagenesis of bacterial luciferase: investigation of the aldehyde binding site. Biochemistry 28, 2684-2689
21. Cline, T. W. and Hastings, J. W. (1972) Mutationally altered bacterial luciferase. Implications for subunit functions. Biochemistry 11, 3359-3370
22. Nicholas, J. T. (2003) Directed evolution of enzymes for applied biocatalysis. Trends Biotechnol. 21, 474-478
23. Cherry, J. R. and Fidantsef, A. L. (2003) Directed evolution of industrial enzymes: an update. Curr. Opin. Biotechnol. 14, 438-443
24. Iffland, I., Tafelmeyer, P., Saudan, C. and Johnsson, K. (2000) Directed evolution of cytochrome c peroxidase. Biochemistry 39, 10790-10798
25. Fisher, A. J., Raushel, F. M., Baldwin, T. O. and Rayment, I. (1995) Three dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4 Å resolution. Biochemistry 34, 6581-6586
26. Lin, L. Y., Sulea, T., Szittner, R., Vassilyev, V., Kor, C., Purisima, E. O. and Meighen, E. A. (2002) Implications of the reactive thiol and the proximal non-proline cis-peptide bond in the structure and function of Vibrio harveyi luciferase. Biochemistry 41, 9938-9945
27. Branchini, B. R., Magyer, R. A., Murtiashaw, M. H., Anderson, S. M., Helgrson, L. C. and Zimmer, M. (1999) Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color. Biochemistry 38, 13223-13230
28. Branchini, B. R., Magyar, R. A., Murtiashaw, M. H., Anderson, S. M. and Zimmer, M. (1998) Site-directed mutagenesis of histidine 245 in firefly luciferase: a proposed model of the active site. Biochemistry 37, 15311-15319
29. Matsumura, I. and Ellington, A. D. (1996) DNA shuffling brightens prospects for GFP. Nat. Biotechnol. 14, 366
30. Crameri, A., Whitehorn, E. A., Tate, E. and Stemmer, W. P. (1996) Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat. Biotechnol. 14, 315-319