Sampling of intracellular metabolites for stationary and non-stationary 13C metabolic flux analysis in Escherichia coli

Analytical Biochemistry

Volumn 465, Issue , 2014, Pages 38-49

  Millard, Pierre ^a,b,c   Massou, Stéphane ^a,b,c   Wittmann, Christoph ^d   Portais, Jean Charles ^a,b,c   Létisse, Fabien ^a,b,c  

^a INSA   (France)

^b UMR792 INGÉNIERIE DES SYSTÈMES BIOLOGIQUES ET DES PROCÉDÉS   (France)

^c CNRS   (France)

^d SAARLAND UNIVERSITY   (Germany)

Author keywords

13C labeling experiments; Isotopes; Isotopologue; Mass isotopomer; Metabolism; Sampling procedures

Indexed keywords

ESCHERICHIA COLI; ISOTOPES; METABOLISM; METABOLITES;

13-C LABELING; INTRACELLULAR METABOLITES; ISOTOPIC INFORMATION; ISOTOPOLOGUES; MASS ISOTOPOMER; METABOLIC FLUX ANALYSIS; METABOLIC INTERMEDIATES; SAMPLING PROCEDURES;

IMPORTANCE SAMPLING;

CARBON 13; SOLVENT; CARBON;

ARTICLE; CALCULATION; CELL METABOLISM; COLD; CONTROLLED STUDY; ESCHERICHIA COLI; FILTRATION; HEATING; ISOTOPE LABELING; KINETICS; METABOLIC FLUX ANALYSIS; NONHUMAN; BIOLOGICAL MODEL; CHEMISTRY; ESCHERICHIA COLI K 12; METABOLISM; METABOLOMICS; PROCEDURES;

CARBON ISOTOPES; ESCHERICHIA COLI K12; ISOTOPE LABELING; METABOLOMICS; MODELS, BIOLOGICAL;

EID: 84907292694     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.07.026     Document Type: Article

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