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Volumn 154, Issue 2-3, 2011, Pages 179-190

Stationary versus non-stationary 13C-MFA: A comparison using a consistent dataset

Author keywords

13C MFA; Isotopic labeling; Metabolic flux analysis

Indexed keywords

13C-MFA; CORYNEBACTERIUM GLUTAMICUM; CURRENT LIMITATION; DATA INTEGRATION; DATA SETS; EQUILIBRIUM DATA; FAST SAMPLING; FED-BATCH CULTIVATION; FLUX ANALYSIS; FLUX DISTRIBUTIONS; INTRACELLULAR METABOLITES; ISOTOPIC LABELING; METABOLIC FLUX ANALYSIS; MODELING APPROACH; NONSTATIONARY; STATIONARITY; STATIONARY STATE; TIME COURSE; TRANSIENT DATA;

EID: 79958703818     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.07.008     Document Type: Article
Times cited : (57)

References (53)
  • 1
    • 35348941242 scopus 로고    scopus 로고
    • Accurate assessment of amino acid mass isotopomer distributions for metabolic flux analysis
    • Antoniewicz M.R., Kelleher J.K., Stephanopoulos G. Accurate assessment of amino acid mass isotopomer distributions for metabolic flux analysis. Anal. Chem. 2007, 79(19):7554-7559.
    • (2007) Anal. Chem. , vol.79 , Issue.19 , pp. 7554-7559
    • Antoniewicz, M.R.1    Kelleher, J.K.2    Stephanopoulos, G.3
  • 2
    • 33845679072 scopus 로고    scopus 로고
    • Elementary metabolite units (EMU): a novel framework for modeling isotopic distributions
    • Antoniewicz M.R., Kelleher J.K., Stephanopoulos G. Elementary metabolite units (EMU): a novel framework for modeling isotopic distributions. Metab. Eng. 2007, 9(1):68-86.
    • (2007) Metab. Eng. , vol.9 , Issue.1 , pp. 68-86
    • Antoniewicz, M.R.1    Kelleher, J.K.2    Stephanopoulos, G.3
  • 3
    • 35348981360 scopus 로고    scopus 로고
    • Metabolic flux engineering of l-lysine production in Corynebacterium glutamicum-over expression and modification of G6P dehydrogenase
    • Becker J., Klopprogge C., Herold A., Zelder O., Bolten C.J., Wittmann C. Metabolic flux engineering of l-lysine production in Corynebacterium glutamicum-over expression and modification of G6P dehydrogenase. J. Biotechnol. 2007, 132(2):99-109.
    • (2007) J. Biotechnol. , vol.132 , Issue.2 , pp. 99-109
    • Becker, J.1    Klopprogge, C.2    Herold, A.3    Zelder, O.4    Bolten, C.J.5    Wittmann, C.6
  • 4
    • 42449109972 scopus 로고    scopus 로고
    • Metabolic responses to pyruvate kinase deletion in lysine producing Corynebacterium glutamicum
    • Becker J., Klopprogge C., Wittmann C. Metabolic responses to pyruvate kinase deletion in lysine producing Corynebacterium glutamicum. Microb. Cell Fact. 2008, 7:8.
    • (2008) Microb. Cell Fact. , vol.7 , pp. 8
    • Becker, J.1    Klopprogge, C.2    Wittmann, C.3
  • 5
    • 27744506402 scopus 로고    scopus 로고
    • Amplified expression of fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources
    • Becker J., Klopprogge C., Zelder O., Heinzle E., Wittmann C. Amplified expression of fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources. Appl. Environ. Microbiol. 2005, 71(12):8587-8596.
    • (2005) Appl. Environ. Microbiol. , vol.71 , Issue.12 , pp. 8587-8596
    • Becker, J.1    Klopprogge, C.2    Zelder, O.3    Heinzle, E.4    Wittmann, C.5
  • 6
    • 34347258175 scopus 로고    scopus 로고
    • Quantitative prediction of cellular metabolism with constraint-based models: the COBRA toolbox
    • Becker S.A., Feist A.M., Mo M.L., Hannum G., Palsson B.Ø., Herrgard M.J. Quantitative prediction of cellular metabolism with constraint-based models: the COBRA toolbox. Nat. Protoc. 2007, 2(3):727-738.
    • (2007) Nat. Protoc. , vol.2 , Issue.3 , pp. 727-738
    • Becker, S.A.1    Feist, A.M.2    Mo, M.L.3    Hannum, G.4    Palsson, B.Ø.5    Herrgard, M.J.6
  • 8
    • 0032981109 scopus 로고    scopus 로고
    • Metabolic state of Zymomonas mobilis in glucose-, fructose-, and xylose-fed continuous cultures as analysed by 13C- and 31P-NMR spectroscopy
    • De Graaf A.A., Striegel K., Wittig R.M., Laufer B., Schmitz G., Wiechert W., Sprenger G.A., Sahm H. Metabolic state of Zymomonas mobilis in glucose-, fructose-, and xylose-fed continuous cultures as analysed by 13C- and 31P-NMR spectroscopy. Arch. Microbiol. 1999, 171(6):371-385.
    • (1999) Arch. Microbiol. , vol.171 , Issue.6 , pp. 371-385
    • De Graaf, A.A.1    Striegel, K.2    Wittig, R.M.3    Laufer, B.4    Schmitz, G.5    Wiechert, W.6    Sprenger, G.A.7    Sahm, H.8
  • 9
    • 0038267105 scopus 로고    scopus 로고
    • Production process monitoring by serial mapping of microbial carbon flux distributions using a novel sensor reactor approach: II-(13)C-labeling-based metabolic flux analysis and l-lysine production
    • Drysch A., El Massaoudi M., Mack C., Takors R., de Graaf A.A., Sahm H. Production process monitoring by serial mapping of microbial carbon flux distributions using a novel sensor reactor approach: II-(13)C-labeling-based metabolic flux analysis and l-lysine production. Metab. Eng. 2003, 5(2):96-107.
    • (2003) Metab. Eng. , vol.5 , Issue.2 , pp. 96-107
    • Drysch, A.1    El Massaoudi, M.2    Mack, C.3    Takors, R.4    de Graaf, A.A.5    Sahm, H.6
  • 10
    • 1442352575 scopus 로고    scopus 로고
    • Serial flux mapping of Corynebacterium glutamicum during fed-batch l-lysine production using the sensor reactor approach
    • Drysch A., El Massaoudi M., Wiechert W., de Graaf A.A., Takors R. Serial flux mapping of Corynebacterium glutamicum during fed-batch l-lysine production using the sensor reactor approach. Biotechnol. Bioeng. 2004, 85(5):497-505.
    • (2004) Biotechnol. Bioeng. , vol.85 , Issue.5 , pp. 497-505
    • Drysch, A.1    El Massaoudi, M.2    Wiechert, W.3    de Graaf, A.A.4    Takors, R.5
  • 11
    • 0038606003 scopus 로고    scopus 로고
    • Production process monitoring by serial mapping of microbial carbon flux distributions using a novel sensor reactor approach: I-sensor reactor system
    • El Massaoudi M., Spelthahn J., Drysch A., de Graaf A., Takors R. Production process monitoring by serial mapping of microbial carbon flux distributions using a novel sensor reactor approach: I-sensor reactor system. Metab. Eng. 2003, 5(2):86-95.
    • (2003) Metab. Eng. , vol.5 , Issue.2 , pp. 86-95
    • El Massaoudi, M.1    Spelthahn, J.2    Drysch, A.3    de Graaf, A.4    Takors, R.5
  • 12
    • 0032735903 scopus 로고    scopus 로고
    • Growth of bacterial cultures' 50 years on: towards an uncertainty principle instead of constants in bacterial growth kinetics
    • Ferenci T. Growth of bacterial cultures' 50 years on: towards an uncertainty principle instead of constants in bacterial growth kinetics. Res. Microbiol. 1999, 150(7):431-438.
    • (1999) Res. Microbiol. , vol.150 , Issue.7 , pp. 431-438
    • Ferenci, T.1
  • 13
    • 1642538406 scopus 로고    scopus 로고
    • High-throughput metabolic flux analysis based on gas chromatography-mass spectrometry derived 13C constraints
    • Fischer E., Zamboni N., Sauer U. High-throughput metabolic flux analysis based on gas chromatography-mass spectrometry derived 13C constraints. Anal. Biochem. 2004, 325(2):308-316.
    • (2004) Anal. Biochem. , vol.325 , Issue.2 , pp. 308-316
    • Fischer, E.1    Zamboni, N.2    Sauer, U.3
  • 14
    • 34547675713 scopus 로고    scopus 로고
    • GC-EI-TOF-MS analysis of in vivo carbon-partitioning into soluble metabolite pools of higher plants by monitoring isotope dilution after 13CO2 labelling
    • Huege J., Sulpice R., Gibon Y., Lisec J., Koehl K., Kopka J. GC-EI-TOF-MS analysis of in vivo carbon-partitioning into soluble metabolite pools of higher plants by monitoring isotope dilution after 13CO2 labelling. Phytochemistry 2007, 68(16-18):2258-2272.
    • (2007) Phytochemistry , vol.68 , Issue.16-18 , pp. 2258-2272
    • Huege, J.1    Sulpice, R.2    Gibon, Y.3    Lisec, J.4    Koehl, K.5    Kopka, J.6
  • 16
    • 33748057502 scopus 로고    scopus 로고
    • Exploiting the bootstrap method for quantifying parameter confidence intervals in dynamical systems
    • Joshi M., Seidel-Morgenstern A., Kremling A. Exploiting the bootstrap method for quantifying parameter confidence intervals in dynamical systems. Metab. Eng. 2006, 8(5):447-455.
    • (2006) Metab. Eng. , vol.8 , Issue.5 , pp. 447-455
    • Joshi, M.1    Seidel-Morgenstern, A.2    Kremling, A.3
  • 17
    • 58249089508 scopus 로고    scopus 로고
    • In silico genome-scale reconstruction and validation of the Corynebacterium glutamicum metabolic network
    • Kjeldsen K.R., Nielsen J. In silico genome-scale reconstruction and validation of the Corynebacterium glutamicum metabolic network. Biotechnol. Bioeng. 2009, 102(2):583-597.
    • (2009) Biotechnol. Bioeng. , vol.102 , Issue.2 , pp. 583-597
    • Kjeldsen, K.R.1    Nielsen, J.2
  • 18
    • 1542376957 scopus 로고    scopus 로고
    • In-depth profiling of lysine-producing Corynebacterium glutamicum by combined analysis of the transcriptome, metabolome, and fluxome
    • Krömer J.O., Sorgenfrei O., Klopprogge K., Heinzle E., Wittmann C. In-depth profiling of lysine-producing Corynebacterium glutamicum by combined analysis of the transcriptome, metabolome, and fluxome. J. Bacteriol. 2004, 186(6):1769-1784.
    • (2004) J. Bacteriol. , vol.186 , Issue.6 , pp. 1769-1784
    • Krömer, J.O.1    Sorgenfrei, O.2    Klopprogge, K.3    Heinzle, E.4    Wittmann, C.5
  • 19
    • 33947503169 scopus 로고    scopus 로고
    • Simultaneous determination of multiple intracellular metabolites in glycolysis, pentose phosphate pathway and tricarboxylic acid cycle by liquid chromatography-mass spectrometry
    • Luo B., Groenke K., Takors R., Wandrey C., Oldiges M. Simultaneous determination of multiple intracellular metabolites in glycolysis, pentose phosphate pathway and tricarboxylic acid cycle by liquid chromatography-mass spectrometry. J. Chromatogr. A 2007, 1147(2):153-164.
    • (2007) J. Chromatogr. A , vol.1147 , Issue.2 , pp. 153-164
    • Luo, B.1    Groenke, K.2    Takors, R.3    Wandrey, C.4    Oldiges, M.5
  • 20
    • 0030050268 scopus 로고    scopus 로고
    • Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing
    • Marx A., de Graaf A.A., Wiechert W., Eggeling L., Sahm H. Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing. Biotechnol. Bioeng. 1996, 49(2):111-129.
    • (1996) Biotechnol. Bioeng. , vol.49 , Issue.2 , pp. 111-129
    • Marx, A.1    de Graaf, A.A.2    Wiechert, W.3    Eggeling, L.4    Sahm, H.5
  • 21
    • 34047177923 scopus 로고    scopus 로고
    • Metabolic flux analysis at ultra short time scale: isotopically non-stationary 13C labeling experiments
    • Nöh K., Grönke K., Luo B., Takors R., Oldiges M., Wiechert W. Metabolic flux analysis at ultra short time scale: isotopically non-stationary 13C labeling experiments. J. Biotechnol. 2007, 129(2):249-267.
    • (2007) J. Biotechnol. , vol.129 , Issue.2 , pp. 249-267
    • Nöh, K.1    Grönke, K.2    Luo, B.3    Takors, R.4    Oldiges, M.5    Wiechert, W.6
  • 22
    • 33749448867 scopus 로고    scopus 로고
    • Computational tools for isotopically instationary 13C labeling experiments under metabolic steady state conditions
    • Nöh K., Wahl S.A., Wiechert W. Computational tools for isotopically instationary 13C labeling experiments under metabolic steady state conditions. Metab. Eng. 2006, 8(6):554-577.
    • (2006) Metab. Eng. , vol.8 , Issue.6 , pp. 554-577
    • Nöh, K.1    Wahl, S.A.2    Wiechert, W.3
  • 23
    • 33744455445 scopus 로고    scopus 로고
    • Experimental design principles for isotopically instationary 13C labeling experiments
    • Nöh K., Wiechert W. Experimental design principles for isotopically instationary 13C labeling experiments. Biotechnol. Bioeng. 2006, 94(2):234-251.
    • (2006) Biotechnol. Bioeng. , vol.94 , Issue.2 , pp. 234-251
    • Nöh, K.1    Wiechert, W.2
  • 25
    • 0027305845 scopus 로고
    • Phosphoenolpyruvate carboxylase in Corynebacterium glutamicum is dispensable for growth and lysine production
    • Peters-Wendisch P.G., Eikmanns B.J., Thierbach G., Bachmann B., Sahm H. Phosphoenolpyruvate carboxylase in Corynebacterium glutamicum is dispensable for growth and lysine production. FEMS Microbiol. Lett. 1993, 112(3):269-274.
    • (1993) FEMS Microbiol. Lett. , vol.112 , Issue.3 , pp. 269-274
    • Peters-Wendisch, P.G.1    Eikmanns, B.J.2    Thierbach, G.3    Bachmann, B.4    Sahm, H.5
  • 27
    • 0035212573 scopus 로고    scopus 로고
    • Metabolic consequences of altered phosphoenolpyruvate carboxykinase activity in Corynebacterium glutamicum reveal anaplerotic regulation mechanisms in vivo
    • Petersen S., Mack C., de Graaf A.A., Riedel C., Eikmanns B.J., Sahm H. Metabolic consequences of altered phosphoenolpyruvate carboxykinase activity in Corynebacterium glutamicum reveal anaplerotic regulation mechanisms in vivo. Metab. Eng. 2001, 3(4):344-361.
    • (2001) Metab. Eng. , vol.3 , Issue.4 , pp. 344-361
    • Petersen, S.1    Mack, C.2    de Graaf, A.A.3    Riedel, C.4    Eikmanns, B.J.5    Sahm, H.6
  • 29
    • 0041931024 scopus 로고    scopus 로고
    • Impact of osmotic stress on volume regulation, cytoplasmic solute composition and lysine production in Corynebacterium glutamicum MH20-22B
    • Rönsch H., Krämer R., Morbach S. Impact of osmotic stress on volume regulation, cytoplasmic solute composition and lysine production in Corynebacterium glutamicum MH20-22B. J. Biotechnol. 2003, 104(1-3):87-97.
    • (2003) J. Biotechnol. , vol.104 , Issue.1-3 , pp. 87-97
    • Rönsch, H.1    Krämer, R.2    Morbach, S.3
  • 30
    • 33846061120 scopus 로고    scopus 로고
    • Metabolic networks in motion: 13C-based flux analysis
    • Sauer U. Metabolic networks in motion: 13C-based flux analysis. Mol. Syst. Biol. 2006, 2:62.
    • (2006) Mol. Syst. Biol. , vol.2 , pp. 62
    • Sauer, U.1
  • 32
    • 67349204820 scopus 로고    scopus 로고
    • Rapid sampling devices for metabolic engineering applications
    • Schädel F., Franco-Lara E. Rapid sampling devices for metabolic engineering applications. Appl. Microbiol. Biotechnol. 2009, 83(2):199-208.
    • (2009) Appl. Microbiol. Biotechnol. , vol.83 , Issue.2 , pp. 199-208
    • Schädel, F.1    Franco-Lara, E.2
  • 33
    • 42249096434 scopus 로고    scopus 로고
    • Metabolic flux analysis as a tool in metabolic engineering of plants
    • Schwender J. Metabolic flux analysis as a tool in metabolic engineering of plants. Curr. Opin. Biotechnol. 2008, 19(2):131-137.
    • (2008) Curr. Opin. Biotechnol. , vol.19 , Issue.2 , pp. 131-137
    • Schwender, J.1
  • 34
    • 33845611853 scopus 로고    scopus 로고
    • Precise metabolic flux analysis of coryneform bacteria by gas chromatography-mass spectrometry and verification by nuclear magnetic resonance
    • Shirai T., Matsuzaki K., Kuzumoto M., Nagahisa K., Furusawa C., Shioya S., Shimizu H. Precise metabolic flux analysis of coryneform bacteria by gas chromatography-mass spectrometry and verification by nuclear magnetic resonance. J. Biosci. Bioeng. 2006, 102(5):413-424.
    • (2006) J. Biosci. Bioeng. , vol.102 , Issue.5 , pp. 413-424
    • Shirai, T.1    Matsuzaki, K.2    Kuzumoto, M.3    Nagahisa, K.4    Furusawa, C.5    Shioya, S.6    Shimizu, H.7
  • 35
    • 0029146299 scopus 로고
    • Biosynthetically directed fractional 13C-labeling of proteinogenic amino acids. An efficient analytical tool to investigate intermediary metabolism
    • Szyperski T. Biosynthetically directed fractional 13C-labeling of proteinogenic amino acids. An efficient analytical tool to investigate intermediary metabolism. Eur. J. Biochem. 1995, 232(2):433-448.
    • (1995) Eur. J. Biochem. , vol.232 , Issue.2 , pp. 433-448
    • Szyperski, T.1
  • 37
  • 38
    • 0037203043 scopus 로고    scopus 로고
    • Cumulative bondomers: a new concept in flux analysis from 2D [13C,1H] COSY NMR data
    • van Winden W.A., Heijnen J.J., Verheijen P.J.T. Cumulative bondomers: a new concept in flux analysis from 2D [13C,1H] COSY NMR data. Biotechnol. Bioeng. 2002, 80(7):731-745.
    • (2002) Biotechnol. Bioeng. , vol.80 , Issue.7 , pp. 731-745
    • van Winden, W.A.1    Heijnen, J.J.2    Verheijen, P.J.T.3
  • 39
    • 15044347498 scopus 로고    scopus 로고
    • Metabolic-flux analysis of Saccharomyces cerevisiae CEN.PK113-7D based on mass isotopomer measurements of (13)C-labeled primary metabolites
    • van Winden W.A., van Dam J.C., Ras C., Kleijn R.J., Vinke J.L., van Gulik W.M., Heijnen J.J. Metabolic-flux analysis of Saccharomyces cerevisiae CEN.PK113-7D based on mass isotopomer measurements of (13)C-labeled primary metabolites. FEMS Yeast Res. 2005, 5(6-7):559-568.
    • (2005) FEMS Yeast Res. , vol.5 , Issue.6-7 , pp. 559-568
    • van Winden, W.A.1    van Dam, J.C.2    Ras, C.3    Kleijn, R.J.4    Vinke, J.L.5    van Gulik, W.M.6    Heijnen, J.J.7
  • 41
    • 2942756043 scopus 로고    scopus 로고
    • Serial 13C-based flux analysis of an l-phenylalanine-producing E. coli strain using the sensor reactor
    • Wahl S.A., Massaoudi M.E., Schipper D., Wiechert W., Takors R. Serial 13C-based flux analysis of an l-phenylalanine-producing E. coli strain using the sensor reactor. Biotechnol. Prog. 2004, 20(3):706-714.
    • (2004) Biotechnol. Prog. , vol.20 , Issue.3 , pp. 706-714
    • Wahl, S.A.1    Massaoudi, M.E.2    Schipper, D.3    Wiechert, W.4    Takors, R.5
  • 42
    • 41049107411 scopus 로고    scopus 로고
    • The topology of metabolic isotope labeling networks
    • Weitzel M., Wiechert W., Noh K. The topology of metabolic isotope labeling networks. BMC Bioinformatics 2007, 8:315.
    • (2007) BMC Bioinformatics , vol.8 , pp. 315
    • Weitzel, M.1    Wiechert, W.2    Noh, K.3
  • 43
    • 68349130318 scopus 로고    scopus 로고
    • Metabolic quenching of Corynebacterium glutamicum: efficiency of methods and impact of cold shock
    • Wellerdiek M., Winterhoff D., Reule W., Brandner J., Oldiges M. Metabolic quenching of Corynebacterium glutamicum: efficiency of methods and impact of cold shock. Bioprocess Biosyst. Eng. 2009, 32(5):581-592.
    • (2009) Bioprocess Biosyst. Eng. , vol.32 , Issue.5 , pp. 581-592
    • Wellerdiek, M.1    Winterhoff, D.2    Reule, W.3    Brandner, J.4    Oldiges, M.5
  • 44
    • 0034115397 scopus 로고    scopus 로고
    • Quantitative determination of metabolic fluxes during coutilization of two carbon sources: comparative analyses with Corynebacterium glutamicum during growth on acetate and/or glucose
    • Wendisch V.F., de Graaf A.A., Sahm H., Eikmanns B.J. Quantitative determination of metabolic fluxes during coutilization of two carbon sources: comparative analyses with Corynebacterium glutamicum during growth on acetate and/or glucose. J. Bacteriol. 2000, 182(11):3088-3096.
    • (2000) J. Bacteriol. , vol.182 , Issue.11 , pp. 3088-3096
    • Wendisch, V.F.1    de Graaf, A.A.2    Sahm, H.3    Eikmanns, B.J.4
  • 45
    • 0034741983 scopus 로고    scopus 로고
    • 13C metabolic flux analysis
    • Wiechert W. 13C metabolic flux analysis. Metab. Eng. 2001, 3(3):195-206.
    • (2001) Metab. Eng. , vol.3 , Issue.3 , pp. 195-206
    • Wiechert, W.1
  • 46
    • 0343267841 scopus 로고    scopus 로고
    • Bidirectional reaction steps in metabolic networks: III. Explicit solution and analysis of isotopomer labeling systems
    • Wiechert W., Möllney M., Isermann N., Wurzel M., de Graaf A.A. Bidirectional reaction steps in metabolic networks: III. Explicit solution and analysis of isotopomer labeling systems. Biotechnol. Bioeng. 1999, 66(2):69-85.
    • (1999) Biotechnol. Bioeng. , vol.66 , Issue.2 , pp. 69-85
    • Wiechert, W.1    Möllney, M.2    Isermann, N.3    Wurzel, M.4    de Graaf, A.A.5
  • 47
    • 0034741985 scopus 로고    scopus 로고
    • A universal framework for 13C metabolic flux analysis
    • Wiechert W., Mollney M., Petersen S., de Graaf A.A. A universal framework for 13C metabolic flux analysis. Metab. Eng. 2001, 3(3):265-283.
    • (2001) Metab. Eng. , vol.3 , Issue.3 , pp. 265-283
    • Wiechert, W.1    Mollney, M.2    Petersen, S.3    de Graaf, A.A.4
  • 48
    • 17144387903 scopus 로고    scopus 로고
    • From stationary to instationary metabolic flux analysis
    • Wiechert W., Nöh K. From stationary to instationary metabolic flux analysis. Adv. Biochem. Eng. Biotechnol. 2005, 92:145-172.
    • (2005) Adv. Biochem. Eng. Biotechnol. , vol.92 , pp. 145-172
    • Wiechert, W.1    Nöh, K.2
  • 49
    • 0031554628 scopus 로고    scopus 로고
    • Bidirectional reaction steps in metabolic networks: II. Flux estimation and statistical analysis
    • Wiechert W., Siefke C., de Graaf A.A., Marx A. Bidirectional reaction steps in metabolic networks: II. Flux estimation and statistical analysis. Biotechnol. Bioeng. 1997, 55(1):118-135.
    • (1997) Biotechnol. Bioeng. , vol.55 , Issue.1 , pp. 118-135
    • Wiechert, W.1    Siefke, C.2    de Graaf, A.A.3    Marx, A.4
  • 50
    • 33847691476 scopus 로고    scopus 로고
    • Fluxome analysis using GC-MS
    • Wittmann C. Fluxome analysis using GC-MS. Microb. Cell Fact. 2007, 6:6.
    • (2007) Microb. Cell Fact. , vol.6 , pp. 6
    • Wittmann, C.1
  • 51
    • 0036956758 scopus 로고    scopus 로고
    • Genealogy profiling through strain improvement by using metabolic network analysis: metabolic flux genealogy of several generations of lysine-producing corynebacteria
    • Wittmann C., Heinzle E. Genealogy profiling through strain improvement by using metabolic network analysis: metabolic flux genealogy of several generations of lysine-producing corynebacteria. Appl. Environ. Microbiol. 2002, 68(12):5843-5859.
    • (2002) Appl. Environ. Microbiol. , vol.68 , Issue.12 , pp. 5843-5859
    • Wittmann, C.1    Heinzle, E.2
  • 52
    • 33747793147 scopus 로고    scopus 로고
    • Respirometric 13C flux analysis-Part II: in vivo flux estimation of lysine-producing Corynebacterium glutamicum
    • Yang T.H., Wittmann C., Heinzle E. Respirometric 13C flux analysis-Part II: in vivo flux estimation of lysine-producing Corynebacterium glutamicum. Metab. Eng. 2006, 8(5):432-446.
    • (2006) Metab. Eng. , vol.8 , Issue.5 , pp. 432-446
    • Yang, T.H.1    Wittmann, C.2    Heinzle, E.3


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