Molecular mechanisms and metabolic engineering of glutamate overproduction in corynebacterium glutamicum

Subcellular Biochemistry

Volumn 64, Issue , 2012, Pages 261-281

  Hirasawa, Takashi ^a   Kim, Jongpill ^a   Shirai, Tomokazu ^b   Furusawa, Chikara ^c   Shimizu, Hiroshi ^a  

^a OSAKA UNIVERSITY   (Japan)

^b RIKEN ^*  (Japan)

^c RIKEN Center for Biosystems Dynamics Research   (Japan)

Author keywords

2 Oxoglutarate dehydrogenase complex; Anaplerotic pathways; Corynebacterium glutamicum; Glutamate; Metabolic engineering; NCgl1221; OdhI

Indexed keywords

2 OXOGLUTARIC ACID; ALPHA-KETOGLUTARIC ACID; GLUTAMIC ACID; METHYLMALONYL COENZYME A DECARBOXYLASE;

BIOSYNTHESIS; CITRIC ACID CYCLE; CORYNEBACTERIUM GLUTAMICUM; GENETICS; METABOLIC ENGINEERING; METABOLISM; PROTEIN SUBUNIT; REVIEW;

CITRIC ACID CYCLE; CORYNEBACTERIUM GLUTAMICUM; GLUTAMIC ACID; KETOGLUTARIC ACIDS; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; METHYLMALONYL-COA DECARBOXYLASE; PROTEIN SUBUNITS;

EID: 84907227592     PISSN: 03060225     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-94-007-5055-5_13     Document Type: Article

References (77)

1. Asakura Y, Kimura E, Usuda Y, Kawahara Y, Matsui K, Osumi T, Nakamatsu T (2007) Altered metabolicflux due to deletion of odhA causes L-glutamate overproduction in Corynebacterium glutamicum. Appl Environ Microbiol 73: 1308-1319.
2. Barthe P, Roumestand C, Canova MJ, Kremer L, Hurard C, Molle V, Cohen-Gonsaud M (2009) Dynamic and structural characterization of a bacterial FHA protein reveals a new autoinhibition mechanism. Structure 17: 568-578.
3. Becker J, Wittmann C (2012) Systems and synthetic metabolic engineering for amino acid production-the heartbeat of industrial strain development. Curr Opin Biotechnol. doi: 10. 1016/j. copbio. 2011. 1012. 1025.
4. Berrier C, Coulombe A, Szabo I, Zoratti M, Ghazi A (1992) Gadolinium ion inhibits loss of metabolites induced by osmotic shock and large stretch-activated channels in bacteria. Eur J Biochem 206: 559-565.
5. Börmann ER, Eikmanns BJ, Sahm H (1992) Molecular analysis of the Corynebacterium glutamicum gdh gene encoding glutamate dehydrogenase. Mol Microbiol 6: 317-326.
6. Börngen K, Battle AR, Moker N, Morbach S, Marin K, Martinac B, Kramer R (2010) The properties and contribution of the Corynebacterium glutamicum MscS variant tofine-tuning of osmotic adaptation. Biochim Biophys Acta 1798: 2141-2149.
7. Boulahya KA, Guedon E, Delaunay S, Schultz C, Boudrant J, Bott M, Goergen JL (2010) OdhI dephosphorylation kinetics during different glutamate production processes involving Corynebacterium glutamicum. Appl Microbiol Biotechnol 87: 1867-1874.
8. Edwards JS, Palsson BO (2000) The Escherichia coli MG1655 in silico metabolic genotype: its definition, characteristics, and capabilities. Proc Natl Acad Sci U S A 97: 5528-5533.
9. Edwards JS, Ibarra RU, Palsson BO (2001) In silico predictions of Escherichia coli metabolic capabilities are consistent with experimental data. Nat Biotechnol 19: 125-130.
10. Eikmanns BJ, Rittmann D, Sahm H (1995) Cloning, sequence analysis, expression, and inactivation of the Corynebacterium glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme. J Bacteriol 177: 774-782.
11. Fudou R, Jojima Y, Seto A, Yamada K, Kimura E, Nakamatsu T, Hiraishi A, Yamanaka S (2002) Corynebacterium efficiens sp. nov., a glutamic-acid-producing species from soil and vegetables. Int J Syst Evol Microbiol 52: 1127-1131.
12. Gopinath V, Meiswinkel TM, Wendisch VF, Nampoothiri KM (2011) Amino acid production from rice straw and wheat bran hydrolysates by recombinant pentose-utilizing Corynebacterium glutamicum. Appl Microbiol Biotechnol 92: 985-996.
13. Hasegawa T, Hashimoto K, Kawasaki H, Nakamatsu T (2008) Changes in enzyme activities at the pyruvate node in glutamate-overproducing Corynebacterium glutamicum. J Biosci Bioeng 105: 12-19.
14. Hashimoto K, Kawasaki H, Akazawa K, Nakamura J, Asakura Y, Kudo T, Sakuradani E, Shimizu S, Nakamatsu T (2006) Changes in composition and content of mycolic acids in glutamate-overproducing Corynebacterium glutamicum. Biosci Biotechnol Biochem 70: 22-30.
15. Hashimoto K, Nakamura K, Kuroda T, Yabe I, Nakamatsu T, Kawasaki H (2010) The protein encoded by NCgl1221 in Corynebacterium glutamicum functions as a mechanosensitive channel. Biosci Biotechnol Biochem 74: 2546-2549.
16. Hirasawa T, Wachi M, Nagai K (2000) A mutation in the Corynebacterium glutamicum ltsA gene causes susceptibility to lysozyme, temperature-sensitive growth, and L-glutamate production. J Bacteriol 182: 2696-2701.
17. Hirasawa T, Wachi M, Nagai K (2001) L-Glutamate production by lysozyme-sensitive Corynebacterium glutamicum ltsA mutant strains. BMC Biotechnol 1: 9.
18. Hoischen C, Kramer R (1990) Membrane alteration is necessary but not sufficient for effective glutamate secretion in Corynebacterium glutamicum. J Bacteriol 172: 3409-3416.
19. Ikeda M, Nakagawa S (2003) The Corynebacterium glutamicum genome: features and impacts on biotechnological processes. Appl Microbiol Biotechnol 62: 99-109.
20. Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Kramer R, Linke B, McHardy AC, Meyer F, Mockel B, Pfefferle W, Puhler A, Rey DA, Ruckert C, Rupp O, Sahm H, Wendisch VF, Wiegrabe I, Tauch A (2003) The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins. J Biotechnol 104: 5-25.
21. Kataoka M, Hashimoto KI, Yoshida M, Nakamatsu T, Horinouchi S, Kawasaki H (2006) Gene expression of Corynebacterium glutamicum in response to the conditions inducing glutamate overproduction. Lett Appl Microbiol 42: 471-476.
22. Kawaguchi H, Vertes AA, Okino S, Inui M, Yukawa H (2006) Engineering of a xylose metabolic pathway in Corynebacterium glutamicum. Appl Environ Microbiol 72: 3418-3428.
23. Kawaguchi H, Sasaki M, Vertes AA, Inui M, Yukawa H (2008) Engineering of an L-arabinose metabolic pathway in Corynebacterium glutamicum. Appl Microbiol Biotechnol 77: 1053-1062.
24. Kawahara Y, Takahashi-Fuke K, Shimizu E, Nakamatsu T, Nakamori S (1997) Relationship between the glutamate production and the activity of 2-oxoglutarate dehydrogenase in Brevibacterium lactofermentum. Biosci Biotechnol Biochem 61: 1109-1112.
25. Kikuchi Y, Date M, Yokoyama K, Umezawa Y, Matsui H (2003) Secretion of active-form Streptoverticillium mobaraense transglutaminase by Corynebacterium glutamicum: processing of the pro-transglutaminase by a cosecreted subtilisin-like protease from Streptomyces albogriseolus. Appl Environ Microbiol 69: 358-366.
26. Kim J, Hirasawa T, Sato Y, Nagahisa K, Furusawa C, Shimizu H (2009) Effect of odhA overexpression and odhA antisense RNA expression on Tween-40-triggered glutamate production by Corynebacterium glutamicum. Appl Microbiol Biotechnol 81: 1097-1106.
27. Kim J, Fukuda H, Hirasawa T, Nagahisa K, Nagai K, Wachi M, Shimizu H (2010) Requirement of de novo synthesis of the OdhI protein in penicillin-induced glutamate production by Corynebacterium glutamicum. Appl Microbiol Biotechnol 86: 911-920.
28. Kim J, Hirasawa T, Saito M, Furusawa C, Shimizu H (2011) Investigation of phosphorylation status of OdhI protein during penicillin-and Tween 40-triggered glutamate overproduction by Corynebacterium glutamicum. Appl Microbiol Biotechnol 91: 143-151.
29. Kimura E, Abe C, Kawahara Y, Nakamatsu T (1996) Molecular cloning of a novel gene, dtsR, which rescues the detergent sensitivity of a mutant derived from Brevibacterium lactofermentum. Biosci Biotechnol Biochem 60: 1565-1570.
30. Kimura E, Abe C, Kawahara Y, Nakamatsu T, Tokuda H (1997) A dtsR gene-disrupted mutant of Brevibacterium lactofermentum requires fatty acids for growth and efficiently produces l-glutamate in the presence of an excess of biotin. Biochem Biophys Res Commun 234: 157-161.
31. Kimura E, Yagoshi C, Kawahara Y, Ohsumi T, Nakamatsu T, Tokuda H (1999) Glutamate overproduction in Corynebacterium glutamicum triggered by a decrease in the level of a complex comprising DtsR and a biotin-containing subunit. Biosci Biotechnol Biochem 63: 1274-1278.
32. Kinoshita S (1985) Glutamic acid bacteria. In: Demain AL, Solomon NA (eds) Biology of industrial microorganisms. Benjamin Cummings, San Francisco, pp 115-145.
33. Kinoshita S, Udaka S, Shimono M (1957) Studies on the amino acid fermentation. Part 1. Production of L-glutamic acid by various microorganisms. J Gen Appl Microbiol 50: 193-205.
34. Kjeldsen KR, Nielsen J (2009) In silico genome-scale reconstruction and validation of the Corynebacterium glutamicum metabolic network. Biotechnol Bioeng 102: 583-597.
35. Krawczyk S, Raasch K, Schultz C, Hoffelder M, Eggeling L, Bott M (2010) The FHA domain of OdhI interacts with the carboxyterminal 2-oxoglutarate dehydrogenase domain of OdhA in Corynebacterium glutamicum. FEBS Lett 584: 1463-1468.
36. Levina N, Totemeyer S, Stokes NR, Louis P, Jones MA, Booth IR (1999) Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. EMBO J 18: 1730-1737.
37. Lv Y, Wu Z, Han S, Lin Y, Zheng S (2011) Genome sequence of Corynebacterium glutamicum S9114, a strain for industrial production of glutamate. J Bacteriol 193: 6096-6097.
38. Lv Y, Liao J, Wu Z, Han S, Lin Y, Zheng S (2012) Genome sequence of Corynebacterium glutamicum ATCC 14067, which provides insight into amino acid biosynthesis in coryneform bacteria. J Bacteriol 194: 742-743.
39. Nakamura J, Hirano S, Ito H, Wachi M (2007) Mutations of the Corynebacterium glutamicum NCgl1221 gene, encoding a mechanosensitive channel homolog, induce L-glutamic acid production. Appl Environ Microbiol 73: 4491-4498.
40. Nakayama K, Yoshida H (1972) Fermentative production of L-arginine. Agric Biol Chem 36: 1675-1684.
41. Nakayama K, Kitada S, Kinoshita S (1961) Studies on lysine fermentation I. The control mechanism on lysine accumulation by homoserine and threonine. J Gen Appl Microbiol 7: 145-154.
42. Nampoothiri KM, Hoischen C, Bathe B, Mockel B, Pfefferle W, Krumbach K, Sahm H, Eggeling L (2002) Expression of genes of lipid synthesis and altered lipid composition modulates L-glutamate efflux of Corynebacterium glutamicum. Appl Microbiol Biotechnol 58: 89-96.
43. Nara T, Samejima H, Kinoshita S (1964) Effect of penicillin on amino acid fermentation. Agric Biol Chem 28: 120-124.
44. Niebisch A, Kabus A, Schultz C, Weil B, Bott M (2006) Corynebacterial protein kinase G controls 2-oxoglutarate dehydrogenase activity via the phosphorylation status of the OdhI protein. J Biol Chem 281: 12300-12307.
45. Nishio Y, Nakamura Y, Kawarabayasi Y, Usuda Y, Kimura E, Sugimoto S, Matsui K, Yamagishi A, Kikuchi H, Ikeo K, Gojobori T (2003) Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens. Genome Res 13: 1572-1579.
46. Oberhardt MA, Palsson BO, Papin JA (2009) Applications of genome-scale metabolic reconstructions. Mol Syst Biol 5: 320.
47. Okino S, Inui M, Yukawa H (2005) Production of organic acids by Corynebacterium glutamicum under oxygen deprivation. Appl Microbiol Biotechnol 68: 475-480.
48. O'Regan M, Thierbach G, Bachmann B, Villeval D, Lepage P, Viret JF, Lemoine Y (1989) Cloning and nucleotide sequence of the phosphoenolpyruvate carboxylase-coding gene of Corynebacterium glutamicum ATCC13032. Gene 77: 237-251.
49. Peters-Wendisch PG, Kreutzer C, Kalinowski J, Patek M, Sahm H, Eikmanns BJ (1998) Pyruvate carboxylase from Corynebacterium glutamicum: characterization, expression and inactivation of the pyc gene. Microbiology 144(Pt 4): 915-927.
50. Peters-Wendisch PG, Schiel B, Wendisch VF, Katsoulidis E, Mockel B, Sahm H, Eikmanns BJ (2001) Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum. J Mol Microbiol Biotechnol 3: 295-300.
51. Radmacher E, Stansen KC, Besra GS, Alderwick LJ, Maughan WN, Hollweg G, Sahm H, Wendisch VF, Eggeling L (2005) Ethambutol, a cell wall inhibitor of Mycobacterium tuberculosis, elicits L-glutamate efflux of Corynebacterium glutamicum. Microbiology 151: 1359-1368.
52. Rittmann D, Lindner SN, Wendisch VF (2008) Engineering of a glycerol utilization pathway for amino acid production by Corynebacterium glutamicum. Appl Environ Microbiol 74: 6216-6222.
53. Sano K, Shiio I (1970) Microbial production of L-lysine III. Production by mutants resistant to S-(2-aminoethyl)-L-cysteine. J Gen Appl Microbiol 16(1): 373-391.
54. Sato H, Orishimo K, Shirai T, Hirasawa T, Nagahisa K, Shimizu H, Wachi M (2008) Distinct roles of two anaplerotic pathways in glutamate production induced by biotin limitation in Corynebacterium glutamicum. J Biosci Bioeng 106: 51-58.
55. Schneider J, Niermann K, Wendisch VF (2011) Production of the amino acids L-glutamate, L-lysine. L-ornithine and L-arginine from arabinose by recombinant Corynebacterium glutamicum. J Biotechnol 154: 191-198.
56. Schultz C, Niebisch A, Gebel L, Bott M (2007) Glutamate production by Corynebacterium glutamicum: dependence on the oxoglutarate dehydrogenase inhibitor protein OdhI and protein kinase PknG. Appl Microbiol Biotechnol 76: 691-700.
57. Schultz C, Niebisch A, Schwaiger A, Viets U, Metzger S, Bramkamp M, Bott M (2009) Genetic and biochemical analysis of the serine/threonine protein kinases PknA, PknB. PknG and PknL of Corynebacterium glutamicum: evidence for non-essentiality and for phosphorylation of OdhI and FtsZ by multiple kinases. Mol Microbiol 74: 724-741.
58. Shigu H, Terui G (1971) Studies on process of glutamic acid fermentation at the enzyme level. I. On the change of α-ketoglutaric acid dehydrogenase in the course of culture. J Ferm Technol 49: 400-405.
59. Shiio I, Nakamori S (1970) Microbial production of L-threonine. Part II. Production by α-amino-β-hydroxyvaleric acid resistant mutants of glutamate producing bacteria. Agric Biol Chem 34: 448-456.
60. Shiio I, Otsuka SI, Takahashi M (1962) Effect of biotin on the bacterial formation of glutamic acid. I. Glutamate formation and cellular premeability of amino acids. J Biochem 51: 56-62.
61. Shimizu H, Hirasawa T (2007) Production of glutamate and glutamate-related amino acids: molecular mechanism analysis and metabolic engineering. In: Wendisch VF (ed) Amino acid biosynthesis-pathways, regulation and metabolic engineering. Microbiology monograph, vol 5. Springer, Berlin, pp 1-38.
62. Shimizu H, Tanaka H, Nakato A, Nagahisa K, Kimura E, Shioya S (2003) Effects of the changes in enzyme activities on metabolicflux redistribution around the 2-oxoglutarate branch in glutamate production by Corynebacterium glutamicum. Bioprocess Biosyst Eng 25: 291-298.
63. Shinfuku Y, Sorpitiporn N, Sono M, Furusawa C, Hirasawa T, Shimizu H (2009) Development and experimental verification of a genome-scale metabolic model for Corynebacterium glutamicum. Microb Cell Fact 8: 43.
64. Shirai T, Nakato A, Izutani N, Nagahisa K, Shioya S, Kimura E, Kawarabayasi Y, Yamagishi A, Gojobori T, Shimizu H (2005) Comparative study offlux redistribution of metabolic pathway in glutamate production by two coryneform bacteria. Metab Eng 7: 59-69.
65. Shirai T, Matsuzaki K, Kuzumoto M, Nagahisa K, Furusawa C, Shioya S, Shimizu H (2006) Precise metabolicflux analysis of coryneform bacteria by gas chromatography-mass spectrometry and verification by nuclear magnetic resonance. J Biosci Bioeng 102: 413-424.
66. Shirai T, Fujimura K, Furusawa C, Nagahisa K, Shioya S, Shimizu H (2007) Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolicflux analysis. Microb Cell Fact 6: 19.
67. Stephanopoulos G, Aristidou AA, Nielsen J (1998) Metabolic engineering: principles and methodologies. Academic, San Diego.
68. Takinami K, Yoshii H, Tsuri H, Okada H (1965) Biochemical effects of fatty acid and its derivatives on L-glutamic acid fermentation. Part III. Biotin-Tween 60 relationship in the accumulation of L-glutamic acid and the growth of Brevibacterium lactofermentum. Agric Biol Chem 29: 351-359.
69. Tsuchida T, Yoshinaga F, Kubota K, Momose H (1975) Production of L-valine by 2-thiazolealanine resistant mutants derived from glutamic acid producing bacteria. Agric Biol Chem 39: 1319-1322.
70. Tsuchidate T, Tateno T, Okai N, Tanaka T, Ogino C, Kondo A (2011) Glutamate production from β-glucan using endoglucanase-secreting Corynebacterium glutamicum. Appl Microbiol Biotechnol 90: 895-901.
71. Udaka S (1960) Screening method for microorganisms accumulating metabolites and its use in the isolation of Micrococcus glutamicus. J Bacteriol 79: 754-755.
72. 13 C metabolicflux analysis. J Biosci Bioeng 112: 595-601.
73. Vallino JJ, Stephanopoulos G (1993) Metabolicflux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol Bioeng 41: 633-646.
74. Wendisch VF, Bott M, Eikmanns BJ (2006) Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for biotechnological production of organic acids and amino acids. Curr Opin Microbiol 9: 268-274.
75. Yao W, Chu C, Deng X, Zhang Y, Liu M, Zheng P, Sun Z (2009a) Display of α-amylase on the surface of Corynebacterium glutamicum cells by using NCgl1221 as the anchoring protein, and production of glutamate from starch. Arch Microbiol 191: 751-759.
76. Yao W, Deng X, Zhong H, Liu M, Zheng P, Sun Z, Zhang Y (2009b) Double deletion of dtsR1 and pyc induce efficient L-glutamate overproduction in Corynebacterium glutamicum. J Ind Microbiol Biotechnol 36: 911-921.
77. Yukawa H, Omumasaba CA, Nonaka H, Kos P, Okai N, Suzuki N, Suda M, Tsuge Y, Watanabe J, Ikeda Y, Vertes AA, Inui M (2007) Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R. Microbiology 153: 1042-1058.